Results 81 to 90 of about 7,101,969 (301)

Characterization of Ricin and R. communis Agglutinin Reference Materials

Toxins, 2015
Ricinus communis intoxications have been known for centuries and were attributed to the toxic protein ricin. Due to its toxicity, availability, ease of preparation, and the lack of medical countermeasures, ricin attracted interest as a potential ...
Sylvia Worbs, Martin Skiba, Martin Söderström, Marja-Leena Rapinoja, Reinhard Zeleny, Heiko Russmann, Heinz Schimmel, Paula Vanninen, Sten-Åke Fredriksson, Brigitte G. Dorner   +9 more
doaj   +1 more source

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Evaluation of the accuracy of laboratories in measuring aflatoxins in foods

Bihdāsht-i Mavādd-i Ghaz̠āyī
The accuracy—encompassing both precision and trueness—of laboratory performance in measuring food toxins, particularly aflatoxins due to their significant impact on human health, is critically important.
Maryam Jalili, Elham Hashemi Shaad
doaj  

Organization and ELISA-Based Results of the First Proficiency Testing to Evaluate the Ability of European Union Laboratories to Detect Staphylococcal Enterotoxin Type B (SEB) in Buffer and Milk

Toxins, 2016
The aim of this work was to organize the first proficiency test (PT) dedicated to staphylococcal enterotoxin B (SEB) detection in milk and buffer solutions.
Yacine Nia, Mélanie Rodriguez, Reinhard Zeleny, Sabine Herbin, Frédéric Auvray, Uwe Fiebig, Marc-André Avondet, Amalia Munoz, Jacques-Antoine Hennekinne   +8 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Latent Print Proficiency Testing: An Examination of Test Respondents, Test‐Taking Procedures, and Test Characteristics [PDF]

, 2019
Proficiency testing is a key component of quality assurance programs within crime laboratories and can help improve laboratory practices. However, current proficiency testing procedures contain significant limitations and can be misinterpreted by ...
Gardner, Brett, Kelley, Sharon, Pan, Karen, Sharon Kelley, Karen D. H. Pan, Brett O. Gardner   +5 more
core   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Evaluation of an Adaptive Learning Technology in a First-year Extended Curriculum Programme Physics course

South African Computer Journal, 2017
Personalised, adaptive online learning platforms that form part of web-based proficiency tests play a major role in the improvement of the quality of learning in physics and assist learners in building proficiency, preparing for tests and using their ...
Moses Mushe Basitere, Eunice Ndeto Ivala
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source