Results 81 to 90 of about 823,140 (217)

Emerging experimental and computational methods for studying redox‐regulated structural transitions

FEBS Letters, EarlyView.
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass, Dana Reichmann, Gábor Erdős   +2 more
wiley   +1 more source

A comparison of modeling approaches for static and dynamic prediction of central-line bloodstream infections using electronic health records (part 1): regression models

Diagnostic and Prognostic Research
Background Hospitals register information in the electronic health records (EHRs) continuously until discharge or death. As such, there is no censoring for in-hospital outcomes.
Shan Gao, Elena Albu, Hein Putter, Pieter Stijnen, Frank E Rademakers, Veerle Cossey, Yves Debaveye, Christel Janssens, Ben Van Calster, Laure Wynants   +9 more
doaj   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

Expert panel as reference standard procedure in diagnostic accuracy studies: a systematic scoping review and methodological guidance

Diagnostic and Prognostic Research
Background In diagnostic accuracy studies, when no reference standard test is available, a group of experts, combined in an expert panel, is often used to assess the presence of the target condition using multiple relevant pieces of patient information ...
Bas E. Kellerhuis, Kevin Jenniskens, Mike P. T. Kusters, Ewoud Schuit, Lotty Hooft, Karel G. M. Moons, Johannes B. Reitsma   +6 more
doaj   +1 more source

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

FEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source

The development and internal validation of a multivariable model predicting 6-month mortality for people with opioid use disorder presenting to community drug services in England: a protocol

Diagnostic and Prognostic Research
Background People with opioid use disorder have substantially higher standardised mortality rates compared to the general population; however, lack of clear individual prognostic information presents challenges to prioritise or target interventions ...
Emmert Roberts, John Strang, Patrick Horgan, Brian Eastwood   +3 more
doaj   +1 more source

A conceptual framework for prognostic research. [PDF]

BMC Med Res Methodol, 2020
Kent P, Cancelliere C, Boyle E, Cassidy JD, Kongsted A.   +4 more
europepmc   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Can we develop real-world prognostic models using observational healthcare data? Large-scale experiment to investigate model sensitivity to database and phenotypes

Diagnostic and Prognostic Research
Background Large observational healthcare databases are frequently used to develop models to be implemented in real-world clinical practice populations.
Jenna M. Reps, Peter R. Rijnbeek, Patrick B. Ryan   +2 more
doaj   +1 more source