Mapping Hsp104 interactions using cross‐linking mass spectrometry
This study examines how cross‐linking mass spectrometry can be utilized to analyze ATP‐induced conformational changes in Hsp104 and its interactions with substrates. We developed an analytical pipeline to distinguish between intra‐ and inter‐subunit contacts within the hexameric homo‐oligomer and discovered contacts between Hsp104 and a selected ...
Kinga Westphal+3 more
wiley +1 more source
Gene mapping and identification of candidate genes controlling carotenoid accumulation of yellow kernels in foxtail millet. [PDF]
Wang J+8 more
europepmc +1 more source
Alkane biosynthesis gene expression and its increased production in recombinant cyanobacteria
The transcription and mRNA structures of the alkane biosynthesis genes ado/aar were analysed in the representative cyanobacteria PCC 6803 and SK1‐2‐1, which produce C17H36 and C15H32, respectively. PCC 6803 transconjugants carrying these genes showed a 1.8‐ to 2.3‐fold increase in C17H36 production.
Misato Nagao+4 more
wiley +1 more source
Drosophila architectural proteins M1BP and Opbp cooperatively form the active promoter of a ribosomal protein gene. [PDF]
Osadchiy I+7 more
europepmc +1 more source
The C. elegans tetraspanin‐7 (tsp‐7) is a homologue of human CD63, which is a negative regulator of autophagy. The C. elegans strain, tm5761, has a dysfunctional (knockout) tsp‐7 gene. When compared to the wild‐type strain, the tm5761 strain shows increased: life‐ and health‐span; thermotolerance, and stress‐induced locomotion.
Brogan Jones+2 more
wiley +1 more source
Decoding G-Quadruplexes Sequence in <i>Vitis vinifera</i>: Regulatory Region Enrichment, Drought Stress Adaptation, and Sugar-Acid Metabolism Modulation. [PDF]
Xie J+8 more
europepmc +1 more source
The tumor microenvironment is a dynamic, multifaceted complex system of interdependent cellular, biochemical, and biophysical components. Three‐dimensional in vitro models of the tumor microenvironment enable a better understanding of these interactions and their impact on cancer progression and therapeutic resistance.
Salma T. Rafik+3 more
wiley +1 more source
Novel tools for genomic modification and heterologous gene expression in the phylum Planctomycetota. [PDF]
Haufschild T+5 more
europepmc +1 more source
SLC7A11 frequently migrates faster in SDS‐PAGE. The present study found that the high hydrophobicity of SLC7A11 causes its anomalous migration in SDS‐PAGE with a low concentration of acrylamide gel. Replacing isoleucine with asparagine reduced hydrophobicity and restored its normal migration at 55 kDa, revealing the role of hydrophobicity and gel ...
Nsengiyumva Emmanuel+13 more
wiley +1 more source
Molecular basis of Streptomyces ECF σShbA factors transcribing principal σHrdB genes. [PDF]
Liu G+5 more
europepmc +1 more source