Results 241 to 250 of about 50,411 (300)

Radioimmunoassay of the F Prostaglandins

The Journal of Clinical Endocrinology & Metabolism, 1971
ABSTRACT Antibodies to prostaglandin F2α (PGF2α) were produced in rabbits immunized with a conjugate of PGF2α covalently linked to bovine serum albumin (BSA) by reaction with carbodiimide reagent. A radioimmunoassay was developed using dextran-coated charcoal to separate the free from antibody-bound PGF2α-3H. The sensitivity of the method was about 100
B V, Caldwell, S, Burstein, W A, Brock, L, Speroff   +3 more
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Intramyometrial prostaglandin F2α

American Journal of Obstetrics and Gynecology, 1972
Abstract Hypertonic saline and prostaglandin F 2α were injected into pregnant monkey myometrium. Small volumes of 20 per cent saline produced full-thickness myometrial necrosis with hemorrhage and thrombosis. PGF 2α in amounts sufficient to induce uterine contractions caused no histologic change.
A C, Wentz, T M, King
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Prostaglandin F2α Receptor-Dependent Regulation of Prostaglandin Transport

Molecular Pharmacology, 2001
Prostaglandin (PG) F(2alpha) may act on its G protein-coupled receptor (FP) or be imported intracellularly via a transporter, which has high affinity for PGF(2alpha) and PGE(2), but not prostacyclin (PGI(2)). In cells overexpressing the epitope-tagged FP together with the human prostaglandin transporter (hPGT), stimulation of the FP with PGF(2alpha) (1
R, Vezza, J, Rokach, G A, FitzGerald
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Amide and l-amino derivatives of F prostaglandins as prostaglandin antagonists

Nature, 1978
THE formation in tissues and organs of the different prostaglandins (PGs) is now known to be associated with various pathophysiological situations. There is an urgent need for specific PG antagonists to facilitate experimental differentiation and analysis of the roles of these endogenous PGs.
Y T, Maddox, P W, Ramwell, C S, Shiner, E J, Corey   +3 more
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Prostaglandin F synthase

Prostaglandins & Other Lipid Mediators, 2002
Prostaglandin (PG) F2 is synthesized via three pathways from PGE2, PGD2, or PGH2 by PGE 9-ketoreductase, PGD 11-ketoreductase, or PGH 9-, 11-endoperoxide reductase, respectively. The enzymological and molecular biological properties of these enzymes have been reported in work over the last 30 years.
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Simple direct radioimmunoassay of the F prostaglandins

Prostaglandins, 1974
Abstract A simplified and accurate method of determining the F prostaglandins in 0.1 ml of serum without previous extraction is described. The procedure involves addition of anti-prostaglandin F2α to serum followed by tritiated prostaglandin, equilibration for 4 hours, removal of unbound prostaglandin with dextran-coated charcoal and subsequent ...
E, Youssefnejadian, E, Walker, I F, Sommerville, I, Craft   +3 more
openaire   +2 more sources

Co-localization of prostaglandin F synthase, cyclooxygenase-1 and prostaglandin F receptor in mouse Leydig cells

Histochemistry and Cell Biology, 2007
In order to promote better understanding of the physiological roles of prostaglandin F(2alpha) in the mouse testis, we investigated the protein expression and the cellular localization of the enzymes cyclooxygenase and prostaglandin F synthase that are essential for the production of prostaglandin F(2alpha), and the binding site, which is the ...
Toshiko, Suzuki-Yamamoto, Yukihiko, Sugimoto, Atsushi, Ichikawa, Kazunori, Ishimura   +3 more
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Agents favoring prostaglandin F formation during biosynthesis

Biochemistry, 1975
The microsomal fraction of bovine vesicular gland catalyzed the conversion of eicosapolyenoic acids exclusively to prostaglandin E in the presence of reduced glutathione, while hydrox fatty acids, prostaglandins D and F, decreased to a negligible level.
J A, Chan, M, Nagasawa, C, Takeguchi, C J, Sih   +3 more
openaire   +2 more sources