Results 31 to 40 of about 8,830,588 (205)
Determining biophysical protein stability in lysates by a fast proteolysis assay, FASTpp. [PDF]
, 2012 The biophysical stability is an important parameter for protein activity both in vivo and in vitro. Here we propose a method to analyse thermal melting of protein domains in lysates: Fast parallel proteolysis (FASTpp).
Rüdiger, Stefan G. D. +10 more
core +3 more sources
Non-Contiguous Protein Recombination [PDF]
, 2013 Swapping sequence elements among related proteins can produce chimeric proteins with novel behaviors and improved properties such as enhanced stability. Although homologous mutations are much more conservative than random mutations, chimeras of distantly-
Smith, Matthew Alexander
core +1 more source
Nature Communications, 2016 Viruses interact with their hosts via secreted and membrane-bound proteins to affect host immune responses and virulence. Here the authors contribute to our understanding of this relationship with an extracellular interaction map of human and adenoviral ...
Nadia Martinez-Martin +11 more
doaj +1 more source
Advances in Nutrition, 2011 Proteins are polymers of amino acids linked via α-peptide bonds. They can be represented as primary, secondary, tertiary, and even quaternary structures, but from a nutritional viewpoint only the primary (amino acid) sequence is of interest. Similarly, although there are many compounds in the body that can be chemically defined as amino acids, we are ...
Malcolm, Watford, Guoyao, Wu
openaire +4 more sources
Analysis of Disulfide Bond Formation [PDF]
, 2017 In this unit, protocols are provided for detection of disulfide bond formation in cultures of intact cells and in an in vitro translation system containing isolated microsomes or semi-permeabilized cells.
Sub Cellular Protein Chemistry +9 more
core +2 more sources
An artificial immune system for clustering amino acids in the context of protein function classification [PDF]
, 2009 This paper addresses the classification task of data mining (a form of supervised learning) in the context of an important bioinformatics problem, namely the prediction of protein functions.
Timmis, Jon +5 more
core +1 more source
Predicting Antibody Developability Profiles Through Early Stage Discovery Screening
mAbs, 2020 Monoclonal antibodies play an increasingly important role for the development of new drugs across multiple therapy areas. The term ‘developability’ encompasses the feasibility of molecules to successfully progress from discovery to development via ...
Marc Bailly +21 more
doaj +1 more source
Communications Biology, 2021 Dreier et al. reports that the anion channelrhodopsin GtACR1 does not undergo a syn-cycle (light adapted ground state) and thus has a more efficient channel behaviour than CrChR2.
Max-Aylmer Dreier +8 more
doaj +1 more source
Calcium as a crucial cofactor for low density lipoprotein receptor folding in the endoplasmic reticulum [PDF]
, 2010 The family of low density lipoprotein (LDL) receptors mediate uptake of a plethora of ligands from the circulation and couple this to signaling, thereby performing a crucial role in physiological processes including embryonic development, cancer ...
Sub Cellular Protein Chemistry +6 more
core +2 more sources
Cryo-EM demonstrates the in vitro proliferation of an ex vivo amyloid fibril morphology by seeding
Nature Communications, 2022 Here, the authors present the cryo-EM structure of in vitro amyloid fibrils from recombinant SAA1.1 protein that were formed by seeding with fibrils purified from systemic AA amyloidosis tissue.
Thomas Heerde +12 more
doaj +1 more source