Results 221 to 230 of about 574,007 (259)

Large-scale functional analysis using peptide or protein arrays

Nature Biotechnology, 2000
The array format for analyzing peptide and protein function offers an attractive experimental alternative to traditional library screens. Powerful new approaches have recently been described, ranging from synthetic peptide arrays to whole proteins expressed in living cells.
A Q, Emili, G, Cagney
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The Use of Electron Tomography for Structural Analysis of Disordered Protein Arrays

Journal of Structural Biology, 1997
A method based on Fourier transforms is described for obtaining a 3-D reconstruction from a paracrystalline object with static disorder. The method is derived from the standard methods used in 3-D reconstruction of 2-D crystals except that all of the Fourier coefficients are used and not just the sampled data from the periodic lattice.
K A, Taylor, J, Tang, Y, Cheng, H, Winkler   +3 more
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Network-based analysis of reverse phase protein array data

2016 IEEE International Conference on Bioinformatics and Biomedicine (BIBM), 2016
In this paper, we introduce a computational method for constructing networks based on reverse phase protein array (RPPA) data to identify complex patterns in protein signaling. The method is applied to phosphoproteomic profiles of basal expression and activation/phosphorylation of 76 key signaling proteins in three breast cancer cell lines (MCF7, LCC1,
Rency S. Varghese, Yiming Zuo 0002, Yi Zhao, Yong-Wei Zhang, Sandra A. Jablonski, Mariaelena Pierobon, Emanuel F. Petricoin, Habtom W. Ressom, Louis M. Weiner   +8 more
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Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array: Storage, Maintenance, and Working with Living Arrays

Cold Spring Harbor Protocols, 2006
INTRODUCTIONThe most difficult aspect of using an array approach for a high-throughput study is maintaining the array’s viability while avoiding contamination of the plates. This protocol describes three different storage methods for the array. Frozen stocks are the most permanent method and are an absolute requirement for maintaining a record of the ...
Tony R, Hazbun, John P, Miller
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Protein Array Based Interactome Analysis of Amyloid-β Indicates an Inhibition of Protein Translation

Journal of Proteome Research, 2011
Oligomeric amyloid-β is currently of interest in amyloid-β mediated toxicity and the pathogenesis of Alzheimer's disease. Mapping the amyloid-β interaction partners could help to discover novel pathways in disease pathogenesis. To discover the amyloid-β interaction partners, we applied a protein array with more than 8100 unique recombinantly expressed ...
Bozso, Zsolt, Virok, Dezso P., Janaky, Tamas, Szegedi, Viktor, Penke, Botond, Fueloep, Livia, Rajko, Robert, Datki, Zsolt, Simon, Dora, Balint, Eva   +9 more
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Analysis of Autoantibody Profiles in Osteoarthritis Using Comprehensive Protein Array Concepts

Journal of Proteome Research, 2014
Osteoarthritis (OA) is the most common rheumatic disease and one of the most disabling pathologies worldwide. To date, the diagnostic methods of OA are very limited, and there are no available medications capable of halting its characteristic cartilage degeneration.
Henjes F, Lourido L, Ruiz-Romero C, Fernández-Tajes J, Schwenk JM, Gonzalez-Gonzalez M, Blanco FJ, Nilsson P, Fuentes M   +8 more
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The Channel Array Interrogation (CAI) instrument for C-reactive protein analysis

2011 International Workshop on Biophotonics, 2011
A Channel Array Interrogation (CAI) instrument for multiparameter-analysis has been developed. In particular, the portable device was optimized for the C-reactive protein (CRP), one of the analytes of interest for sepsis.
AGiannetti, C Trono, F Baldini, L Bolzoni, G Porro   +4 more
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Analysis of Protein Interactions with Immobilized Peptide Arrays Synthesized on Membrane Supports

Cold Spring Harbor Protocols, 2006
INTRODUCTIONThe following protocol describes the synthesis of short linear peptides, or peptide pools, on modified cellulose membranes, and the detection of their protein-binding partners. Peptides are synthesized from their carboxyl termini using Fmoc-amino acid derivatives.
Ronald, Frank, Stefan, Dübel
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Clustering and Network Analysis of Reverse Phase Protein Array Data

2017
Molecular profiling of proteins and phosphoproteins using a reverse phase protein array (RPPA) platform, with a panel of target-specific antibodies, enables the parallel, quantitative proteomic analysis of many biological samples in a microarray format.
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Protein analysis by isoelectric focusing in a capillary array with an absorption imaging detector

Journal of Chromatography B: Biomedical Sciences and Applications, 1995
Isoelectric focusing (IEF) was successfully performed in capillary arrays with up to four capillaries. Separated proteins in the capillary array were detected by an UV absorption imaging detector. The whole analysis time for all samples in the capillary array was only 3 min due to the real-time imaging detector.
J, Wu, J, Pawliszyn
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