Results 271 to 280 of about 2,014,238 (321)
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Single-Molecule Arrays for Protein and Nucleic Acid Analysis
Annual Review of Analytical Chemistry, 2017The last few years have seen breakthroughs that will transform our ability to measure important analytes. Miniaturization of reaction volumes and confinement of analytes of interest into ultrasmall containers have greatly enhanced the sensitivity and throughput of many detection methods. Fabrication of microwell arrays and implementation of bead-based
Limor, Cohen, David R, Walt
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Experimental Design & Analysis of Protein Array Data: Applying Methods from cDNA Arrays.
Blood, 2004Abstract It appears that a number of recent manuscripts using protein microarray technology are using equivalent analysis procedures that the gene-expression microarray community implemented in their infancy. That is, utilizing a classic reference design such that the ratio of the sample of interest to a reference sample is the response ...
Irene Ghobrial +2 more
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Protein chip array profiling analysis of sera from neuroblastoma patients
Cancer Letters, 2005Neuroblastoma, the most common extracranial solid tumour in children, is characterised by highly heterogeneous clinical behaviour; patients are stratified into risk categories according to a combination of clinical and biological markers. However, identifying non-invasive prognostic markers predicting outcome independently from current risk ...
Valérie, Combaret +6 more
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Network-based analysis of reverse phase protein array data
2016 IEEE International Conference on Bioinformatics and Biomedicine (BIBM), 2016In this paper, we introduce a computational method for constructing networks based on reverse phase protein array (RPPA) data to identify complex patterns in protein signaling. The method is applied to phosphoproteomic profiles of basal expression and activation/phosphorylation of 76 key signaling proteins in three breast cancer cell lines (MCF7, LCC1,
Rency S Varghese +8 more
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Large-Scale Analysis of Protein–Protein Interactions Using Cellulose-Bound Peptide Arrays
2008Peptide arrays for screening large numbers of peptide fragments and probing with large numbers of samples is discussed.
Ulrike, Beutling +3 more
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The Channel Array Interrogation (CAI) instrument for C-reactive protein analysis
2011 International Workshop on Biophotonics, 2011A Channel Array Interrogation (CAI) instrument for multiparameter-analysis has been developed. In particular, the portable device was optimized for the C-reactive protein (CRP), one of the analytes of interest for sepsis.
AGiannetti +4 more
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Analysis of Protein–Protein Interactions Using Array-Based Yeast Two-Hybrid Screens
2009The yeast two-hybrid system (Y2H) is a powerful tool to identify protein-protein interactions. Here we describe array-based two-hybrid methods that use defined libraries of open reading frames (ORFs) as opposed to random genomic or cDNA libraries. The array-based Y2H system is well suited for interactome studies of existing ORFeomes or subsets thereof,
Seesandra V, Rajagopala, Peter, Uetz
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Cold Spring Harbor Protocols, 2006
INTRODUCTIONThe most difficult aspect of using an array approach for a high-throughput study is maintaining the array’s viability while avoiding contamination of the plates. This protocol describes three different storage methods for the array. Frozen stocks are the most permanent method and are an absolute requirement for maintaining a record of the ...
Tony R, Hazbun, John P, Miller
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INTRODUCTIONThe most difficult aspect of using an array approach for a high-throughput study is maintaining the array’s viability while avoiding contamination of the plates. This protocol describes three different storage methods for the array. Frozen stocks are the most permanent method and are an absolute requirement for maintaining a record of the ...
Tony R, Hazbun, John P, Miller
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Clustering and Network Analysis of Reverse Phase Protein Array Data
2017Molecular profiling of proteins and phosphoproteins using a reverse phase protein array (RPPA) platform, with a panel of target-specific antibodies, enables the parallel, quantitative proteomic analysis of many biological samples in a microarray format.
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Fluorescence-Based Analysis of Cellular Protein Lysate Arrays Using Quantum Dots
2007Reverse-phase protein microarrays (RPPMAs) enable heterogeneous mixtures of proteins from cellular extracts to be directly spotted onto a substrate (such as a protein biochip) in minute volumes (nanoliter-to-picoliter volumes). The protein spots can then be probed with primary antibodies to detect important posttranslational modifications such as ...
David H, Geho +5 more
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