Results 51 to 60 of about 4,121,048 (281)
High-efficiency screening of monoclonal antibodies for membrane protein crystallography. [PDF]
, 2011 Determination of crystal structures of membrane proteins is often limited by difficulties obtaining crystals diffracting to high resolution. Co-crystallization with Fab fragments of monoclonal antibodies has been reported to improve diffraction of ...
Lim Hyun-Ho +5 more
core +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
FEBS Open Bio, EarlyView.Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
FEBS Open BioThe SARS‐CoV‐2 Nucleocapsid (N) protein plays a crucial role in genome packaging, replication, transcription, and pathogenesis, making it a promising target for antiviral drug development.
Shweta Singh, Gagan D. Gupta
doaj +1 more source
Protein Dynamics and its Correlation to Protein Activity and Stability [PDF]
, 2009 The aim of this thesis is to investigate the role of fast protein dynamics (picosecond timescale) in enzyme activity and stability, and specifically to test the hypothesis that enzyme activity and stability are inversely related by their internal ...
Clement, David
core
The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity
FEBS Open Bio, EarlyView.Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav +4 more
wiley +1 more source
Structural vulnerability in EPCR suggests functional modulation
Scientific ReportsThe endothelial protein C receptor (EPCR) is a fundamental component of the vascular system in mammals due to its contribution in maintaining blood in a non-prothrombotic state, which is crucial for overall life development.
Elena Erausquin +5 more
doaj +1 more source
mBio, 2014 Response regulators are proteins that undergo transient phosphorylation, connecting specific signals to adaptive responses. Remarkably, the molecular mechanism of response regulator activation remains elusive, largely because of the scarcity of ...
Felipe Trajtenberg +10 more
doaj +1 more source
Protein crystallography: alive and well [PDF]
The FEBS Journal, 2021 The motto of this Virtual Issue of The FEBS Journal is a paraphrase of the statement made in 1897 by Mark Twain, which is usually quoted as ‘Reports of my death have been greatly exaggerated’. With the incredible progress in the utilization of cryo‐EM for the determination of high‐resolution macromolecular structures that led to the award of the Nobel ...
openaire +2 more sources
Structure determination of membrane proteins by electron crystallography [PDF]
, 2007 A fundamental principle of life is the separation of environments into different compartments. Prokaryotes shield their interior from the environment by a plasma membrane and in some cases also by a cell wall.
Schenk, Andreas Daniel
core +1 more source
Advanced Functional Materials, EarlyView.Inspired by metal‐free carbonic anhydrases (CAs) that achieve efficient catalysis without metal cofactors, we herein report a sequence‐defined supramolecular peptoid platform that mimics metal‐free CAs and exhibits hydrolytic activity via a supramolecular assembly‐mediated proton‐transfer mechanism.
Progyateg Chakma +8 more
wiley +1 more source