Results 71 to 80 of about 103,093 (251)

A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers. [PDF]

Nat Commun, 2023
Wranik M, Kepa MW, Beale EV, James D, Bertrand Q, Weinert T, Furrer A, Glover H, Gashi D, Carrillo M, Kondo Y, Stipp RT, Khusainov G, Nass K, Ozerov D, Cirelli C, Johnson PJM, Dworkowski F, Beale JH, Stubbs S, Zamofing T, Schneider M, Krauskopf K, Gao L, Thorn-Seshold O, Bostedt C, Bacellar C, Steinmetz MO, Milne C, Standfuss J.   +29 more
europepmc   +2 more sources

Biocrystallography in Switzerland: Achievements and Future Perspectives

CHIMIA, 2014
The first protein crystallography group in Switzerland was installed at the Biozentrum of the University of Basel approximately 40 years ago.
Markus G. Grütter
doaj   +1 more source

Lipid Pocket Binders Impose Allosteric Changes of Protein Dynamics Around the Active Site of the Protein Kinase p38α

Angewandte Chemie International Edition, EarlyView.
Used in search of novel allosteric sites in the serine/threonine kinase p38α, NMR spectroscopy reveals a dynamic coupling between the catalytic and lipid pockets. The findings uncover a motional interdependence that links distant regions of the enzyme, highlighting the lipid pocket as a promising site for allosteric intervention.
Sara Medina Gómez, Laurin T. Homberg, Mike Bührmann, Daniel Rauh, Rasmus Linser   +4 more
wiley   +1 more source

More Than a Buffer in Biochemistry: Tris as an Architect and Gatekeeper of Metal–Oxo Assembly

Angewandte Chemie International Edition, EarlyView.
Tris(hydroxymethyl)aminomethane, depicted as an octopus, acts as an alkoxy ligand, chelator, and structure‐directing buffer that programs polyoxometalate's speciation, nucleation, and heterometal insertion in aqueous solution. ABSTRACT Polyoxometalates (POMs, molecular metal–oxo clusters) are typically studied and applied in aqueous media, where ...
Nadiia I. Gumerova, Annette Rompel
wiley   +1 more source

Neutron Protein Crystallography

Nihon Kessho Gakkaishi, 1996
The structure-function relationship of proteins is dominated by the behavior of hydrogen atoms. Neutron diffraction provides an experimental method of directly locating hydrogen atoms. We have constructed the diffractometer (BIX) dedicated for the neutron crystallography in biology at JRR-3M in Japan Atomic Energy Research Institute.
openaire   +2 more sources

Pseudomonas aeruginosa gene PA4880 encodes a Dps-like protein with a Dps fold, bacterioferritin-type ferroxidase centers, and endonuclease activity

Frontiers in Molecular Biosciences
We report the biochemical, structural, and functional characterization of the protein coded by gene PA4880 in the P. aeruginosa PAO1 genome. The PA4880 gene had been annotated as coding a probable bacterioferritin.
Nimesha Rajapaksha, Huili Yao, Aisha Cook, Steve Seibold, Lijun Liu, Kevin P. Battaile, Leo Fontenot, Fabrizio Donnarumma, Scott Lovell, Mario Rivera   +9 more
doaj   +1 more source

A Multiepitope‐Based Lateral Flow Assay for Leprosy Antibodies

Analysis &Sensing, EarlyView.
An innovative lateral flow assay for leprosy diagnosis is based on gold nanoparticles and synthetic Mycobacterium leprae epitopes conjugated to keyhole limpet hemocyanin protein to enhance antibody detection. This strategy provides a rapid, accurate, and low‐cost approach for resource‐limited settings, enabling the early detection of infection. Leprosy
Cristiane Zocatelli‐Ribeiro, Camila dos Anjos Proença, Ana Carolina da Rosa Nogarolli, Ana Fabiele Domakoski Trevisan, Thiago Neves Machado, João Carlos Degraf Muzzi, Larissa Magalhães Alvarenga, Juliana de Moura   +7 more
wiley   +1 more source

Radium Speciation in Simulated Digestive Biofluids: Insights from Electrospray Ionization Mass Spectrometry and Thermodynamic Modeling

Analysis &Sensing, EarlyView.
Electrospray ionization mass spectrometry (ESI‐MS) combined with thermodynamic modeling provides the first direct characterization of radium speciation in synthetic gastrointestinal biofluids. Free Ra2+ ions predominate with minimal protein binding and negligible EDTA complexation. Crown ethers enable effective Ra complexation.
Linus Holtmann, Tim Schmalz, Tobias Weissenborn, Felix Stäger, Beate Riebe, Clemens Walther   +5 more
wiley   +1 more source

Using structural genomics depositions in undergraduate teaching of protein crystallography: everybody wins. [PDF]

Acta Crystallogr F Struct Biol Commun, 2023
Agirre J.
europepmc   +1 more source

Structures of Helicobacter pylori C-terminal protease CtpA reveal a new mode of self-contained proteolytic processing

Communications Biology
Bacterial carboxyl-terminal processing proteases (CTPs) are essential for protein quality control, signal transduction, and cell adaptation. Typically, activation of CTPs involves substrate binding to the PDZ domain or an adaptor protein.
Kailei Sun, Lili Yan, Chin Yu Mok, Pui Kin So, Sirius Pui Kam Tse, Kwok Fai Lau, Daping Wang, Huawei Zhang, Shannon Wing Ngor Au   +8 more
doaj   +1 more source