Asia-Pacific Journal of Chemical Engineering, EarlyView.ABSTRACT Ag–Fe bimetallic nanoparticles (alloy and core–shell structures) were synthesized using Grewia optiva leaf extract through a green, phytochemical‐mediated approach. The effects of pH, temperature, extract concentration, and Ag:Fe molar ratio on nanoparticle formation were systematically optimized, enabling size‐controlled and morphologically ...
Sehrish Asad +6 more
wiley +1 more source
High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography. [PDF]
J Vis Exp, 2023 Budziszewski GR +4 more
europepmc +1 more source
The time revolution in macromolecular crystallography
Structural DynamicsMacromolecular crystallography has historically provided the atomic structures of proteins fundamental to cellular functions. However, the advent of cryo-electron microscopy for structure determination of large and increasingly smaller and flexible ...
Georgii Khusainov +2 more
doaj +1 more source
Recent Advances in Chemical Probing Strategies for RNA Structure Determination in Vivo
Chemistry – A European Journal, EarlyView.This Review illustrates how structural probes combined with advanced sequencing technologies are revolutionizing our ability to map RNA structures and interactions within living cells. It highlights the development of innovative strategies tailored to probe RNA structures in particular biological contexts.
Maryana Yarshova +2 more
wiley +1 more source
Mix-and-extrude: high-viscosity sample injection towards time-resolved protein crystallography. [PDF]
J Appl Crystallogr, 2023 Vakili M +18 more
europepmc +1 more source
Development of a humanized anti-FABP4 monoclonal antibody for potential treatment of breast cancer
Breast Cancer ResearchBackground Breast cancer is the most common cancer in women diagnosed in the U.S. and worldwide. Obesity increases breast cancer risk without clear underlying molecular mechanisms.
Jiaqing Hao +9 more
doaj +1 more source
Structural basis for the assembly of the Ragulator-Rag GTPase complex
Nature Communications, 2017 mTORC1 activity is controlled through Rag GTPases, which are anchored to the lysosome through the Ragulator. Here, the authors give molecular insights into Ragulator-Rag GTPase assembly and present the crystal structures of the Ragulator alone and in ...
Ryo Yonehara +12 more
doaj +1 more source
Mini‐Hairpin DNA: Extraordinarily Stable Structure and Its Applications
Chemistry – A European Journal, EarlyView.Mini‐hairpin DNA fragments containing GCGAAGC and GCGAAAGC form exceptionally stable hairpin‐like structures, with only two G−C pairs and one sheared G−A pair, remaining undenatured in 7 M urea and resisting nuclease digestion. These mini‐hairpin sequences frequently appear in replication and transcription origins, influence DNA sequencing fidelity ...
Michiko Kimoto, Ichiro Hirao
wiley +1 more source
Identification of Natural Isonitriles Through Ligation to an Azomethine Imine Probe
Chemistry – A European Journal, EarlyView.The azomethine imine (AMI)–isonitrile (NC) ligation allowed for the chemoselective detection of natural isonitriles in bacteria and fungi. The reactivity‐based screening establishes a new stereogenic center, thereby allowing for facile distinction between chiral and achiral isonitriles. The work also unraveled a unique reactivity of isonitriles bearing
Maurice P. Biedermann +6 more
wiley +1 more source
Roles of inter- and intramolecular tryptophan interactions in membrane-active proteins revealed by racemic protein crystallography. [PDF]
Commun Chem, 2023 Lander AJ +6 more
europepmc +1 more source