Results 91 to 100 of about 1,856,978 (353)

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Combinatorial ubiquitin code degrades deubiquitylation-protected substrates

Nature Communications
Protein ubiquitylation is maintained by a dynamic balance of the conjugation and deconjugation of ubiquitin. It remains unclear how deubiquitylation-stabilized substrates are directed for degradation.
Mai Morita, Miyu Takao, Honoka Tokuhisa, Ryotaro Chiba, Shota Tomomatsu, Yoshino Akizuki, Takuya Tomita, Akinori Endo, Yasushi Saeki, Yusuke Sato, Fumiaki Ohtake   +10 more
doaj   +1 more source

Targeting MYOF suppresses pancreatic ductal adenocarcinoma progression by inhibiting ILF3-LCN2 signaling through disrupting OTUB1-mediated deubiquitination of ILF3

Redox Biology
Pancreatic ductal adenocarcinoma (PDAC) is still a highly aggressive and fatal disease. The molecular mechanisms for PDAC progression are still not fully understood.
Zhihui Li, Jianlei Zhang, Jiang Yin, Wen Ma, Hongfan Liao, Lv Ling, Qingfeng Zou, Yabing Cao, Ying Song, Guopei Zheng, Xiaoye Hu, Guohua Yang, Nan Li   +12 more
doaj   +1 more source

Cell density–dependent nuclear‐cytoplasmic shuttling of SETDB1 integrates with Hippo signaling to regulate YAP1‐mediated transcription

FEBS Letters, EarlyView.
At low cell density, SETDB1 and YAP1 accumulate in the nucleus. As cell density increases, the Hippo pathway is gradually activated, and SETDB1 is associated with increased YAP1 phosphorylation. At high cell density, phosphorylated YAP1 is sequestered in the cytoplasm, while SETDB1 becomes polyubiquitinated and degraded by the ubiquitin–proteasome ...
Jaemin Eom, Jaewoong Jang, Jung Sun Park, Yong‐Kook Kang   +3 more
wiley   +1 more source

Notch signaling sustains the expression of Mcl-1 and the activity of eIF4E to promote cell survival in CLL [PDF]

, 2015
In chronic lymphocytic leukemia (CLL), Notch1 and Notch2 signaling is constitutively activated and contributes to apoptosis resistance. We show that genetic inhibition of either Notch1 or Notch2, through small-interfering RNA, increases apoptosis of CLL ...
Baldoni, Stefano, De Falco, Filomena, Del Papa, Beatrice, Di Ianni, Mauro, Falzetti, Franca, Marconi, Pierfrancesco, Rosati, Emanuela, Sabatini, Rita, Screpanti, Isabella, Sportoletti, Paolo   +9 more
core   +3 more sources

β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1

Molecular Oncology, EarlyView.
Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero, Alejandro Belmonte‐Fernández, Mónica González‐Moreno, Laura Rodríguez‐Cordero, Begoña Pérez‐Valderrama, Joaquín Herrero‐Ruíz, Francisco Romero, Carmen Sáez, Miguel Á. Japón   +8 more
wiley   +1 more source

Autophagy Induced by Proteasomal DUB Inhibitor NiPT Restricts NiPT-Mediated Cancer Cell Death

Frontiers in Oncology, 2020
Ubiquitin–proteasome system (UPS) and autophagy–lysosome pathway (ALP) are two major systems for protein quality control (PQC) in eukaryotic cells. Interconnectivity between these two pathways has been suggested, but the molecular detail of how they ...
Jinghong Chen, Jinghong Chen, Xin Chen, Dacai Xu, Li Yang, Li Yang, Zhenjun Yang, Qianqian Yang, Ding Yan, Peiquan Zhang, Du Feng, Jinbao Liu   +11 more
doaj   +1 more source

Discovery of cellular regulation by protein degradation [PDF]

, 2008
What follows is a story of some of the lab’s adventures mentioned above, including the inventions of new biochemical and genetic methods. This account stems, in part, from previous descriptions of the early history of the Ub field (31,32).
Alagramam, Alexander Varshavsky, Alfageme, An, Aulner, Bachmair, Bachmair, Bakayev, Baker, Baker, Balzi, Barsoum, Bartel, Bartel, Blattes, Byrd, Chau, Ciechanover, Ciechanover, Ciechanover, Clarke, Coelho, D'Ambrosio, Damelin, Davydov, Demartino, Deming, Dinardo, Ditzel, Dohmen, Dohmen, Dohmen, Du, Dunnwald, Evans, Ferber, Finley, Finley, Finley, Fried, Garner, Geiss-Friedlander, Gisler, Glotzer, Goebl, Goldknopf, Gonda, Goto, Graciet, Grigoryev, Haering, Haggarty, Hanna, Harper, Hauf, Hershko, Hershko, Hershko, Hershko, Hershko, Hicke, Hochstrasser, Hochstrasser, Holm, Hou, Hu, Hu, Hu, Huang, Hunter, Inobe, Jentsch, Johnson, Johnson, Johnson, Johnsson, Johnsson, Kaiji, Kerppola, Kerscher, Kornberg, Kwon, Kwon, Kwon, Kwon, Kwon, Lee, Levinger, Levinger, Levinger, Levinger, Li, Lou, Lévy, Marunouchi, Matsumoto, McGrath, Meyer-Almes, Milutinovich, Minsky, Mogk, Mukhopadhyay, Nasmyth, Peters, Pickart, Rao, Roninson, Rubinsztein, Rumpf, Sasaki, Shamu, Sheng, Shintomi, Shrader, Skoufias, Soffer, Solomon, Steck, Strauss, Sundin, Sundin, Suzuki, Tarassov, Tasaki, Tasaki, Tobias, Tobias, Turner, Turner, Uemura, Uhlmann, Varshavsky, Varshavsky, Varshavsky, Varshavsky, Varshavsky, Varshavsky, Varshavsky, Varshavsky, Varshavsky, Varshavsky, Varshavsky, Varshavsky, Varshavsky, Varshavsky, Varshavsky, Varshavsky, Wang, Wilkinson, Winter, Wittke, Woelk, Xia, Xie, Xie, Yi, Zenker, Özkaynak, Özkaynak   +158 more
core   +4 more sources

Nicotinamide N‐methyltransferase promotes drug resistance in lung cancer, as revealed by nascent proteomic profiling

Molecular Oncology, EarlyView.
AZD9291 has shown promise in targeted cancer therapy but is limited by resistance. In this study, we employed metabolic labeling and LC–MS/MS to profile time‐resolved nascent protein perturbations, allowing dynamic tracking of drug‐responsive proteins. We demonstrated that increased NNMT expression is associated with drug resistance, highlighting NNMT ...
Zhanwu Hou, Zhen Wang, Fei Yang, Xiao Han, Lei Li, Huadong Liu   +5 more
wiley   +1 more source

Cop1 constitutively regulates c-Jun protein stability and functions as a tumor suppressor in mice [PDF]

, 2011
Biochemical studies have suggested conflicting roles for the E3 ubiquitin ligase constitutive photomorphogenesis protein 1 (Cop 1; also known as Rfwd2) in tumorigenesis, providing evidence for both the oncoprotein c-Jun and the tumor suppressor p53 as ...
Aleksandra Zwolinska, Angel, Baert, Bannister, Chang, Chen, Danovi, de Launoit, Dentin, Derijard, Dieter Defever, Domenico Migliorini, Dornan, Edwards, Eferl, Enrico Radaelli, Fuchs, Geertrui Denecker, Horn, Jean-Christophe Marine, Karin, Kato, Kominami, Krummel, Kyriakis, Lopez-Bergami, Mariani, Marine, Mendrysa, Morse HC, Nakayama, Ouyang, Qi, Rajesh Vyas, Ramaswamy, Rincon, Rossant, Schreiber, Sven Bogaerts, Tino Hochepied, Vanessa Depaepe, Wang, William C. Skarnes, Zhong   +43 more
core   +2 more sources