Results 101 to 110 of about 1,195,384 (219)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Supramolecular Degraders: An Emerging Paradigm in Targeted Protein Degradation. [PDF]

Adv Sci (Weinh)
Liu K, Yuan X, Zhou Y, Yuan R, Xie S, Chen Y, Wang P, Gao J, Gong Q.   +8 more
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Nanotechnology-Enabled Targeted Protein Degradation: Strategies, Opportunities, and Challenges. [PDF]

Small Methods
Zhu H, Zhang Y, Ning Y, Yang C, Zhang G, Zhu Z.   +5 more
europepmc   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Mechanistic Insights into the Dual Directing Group-Mediated C–H Functionalization/Annulation via a Hydroxyl Group-Assisted MIII-MV‑MIII Pathway

ACS Omega, 2021
Huiying Xu, Mengyao Bian, Zhi Zhou, Hui Gao, Wei Yi   +4 more
doaj   +1 more source

Introduction to the thematic review series on intracellular protein degradation. The ubiquitous biology of intracellular protein degradation: A tribute to Alfred L. ("Fred") Goldberg. [PDF]

J Biol Chem
DeMartino GN.
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Correction to: CHD1L prevents lipopolysaccharide-induced hepatocellular carcinomar cell death by activating hnRNP A2/B1-nmMYLK axis

Cell Death and Disease, 2022
Guangliang Wang, Xiaofeng Zhang, Wei Cheng, Yanxuan Mo, Juan Chen, Zhiming Cao, Xiaogang Chen, Huiqin Cui, Shanshan Liu, Li Huang, Ming Liu, Lei Ma, Ning-Fang Ma   +12 more
doaj   +1 more source

Optimizing CRBN-ligands for optical targeted protein degradation. [PDF]

RSC Med Chem
Flores JA, DiNardi RG, Burslem GM, Cheng T, Trauner DH.   +4 more
europepmc   +1 more source