Results 91 to 100 of about 414,168 (349)

Investigating the Cellular Effects of GALC Dosing in Enzyme Replacement Therapy for Krabbe Disease Supports the Role of Nanomedicine

Advanced Biology, EarlyView.
A detailed workflow for recombinant GALC production and characterization is presented to support enzyme replacement therapy for Krabbe disease. In vitro assays demonstrate that physiological GALC doses restore enzymatic activity and autophagic flux without affecting cell viability, whereas higher doses impair autophagy and reduce viability.
Ambra Del Grosso, Sara Carpi, Laura Colagiorgio, Miriam De Sarlo, Mariacristina Gagliardi, Marco Cecchini   +5 more
wiley   +1 more source

Effect of Chaotropic Reagents on Bovine Serum Albumin (BSA) - A Fluorescence Study [PDF]

, 2011
The comparative study of the chemical denaturation by Chaotropic reagents urea and guanidine hydrochloride was studied on the model protein Bovine Serum Albumin (BSA) using UV-Vis Spectrophotometry and Steady State Fluorescence Spectroscopy.
Mishra, Smruti Snigdha
core  

Thermal and chemical unfolding and refolding of a eukaryotic sodium channel [PDF]

, 2009
Voltage-gated sodium channels are dynamic membrane proteins essential for signaling in nervous and muscular systems. They undergo substantial conformational changes associated with the closed, open and inactivated states.
A.O. O'Reilly, Alexandrov, B.A. Wallace, Baneres, Booth, Combet, Cormier, Cronin, Cronin, Cuello, Deerfield, Deleage, Doyle, Evans, Galka, Hubbard, James, Jiang, Kalypso Charalambous, Keynes, Krishnan, Lees, Lentz, Lockless, MacKinnon, Mao, Miles, Noda, Nurani, O'Reilly, Penzotti, Per A. Bullough, Provencher, Renart, Renthal, Riley, Sato, Schneider, Sehgal, Sreerama, Thompson, Tikhonov, von Ballmoos, Whitmore, Wilkins, Yu, Zhang   +46 more
core   +2 more sources

Alleviation of Aging‐Related Hallmarks in a Mouse Model of Progeria via a Nanoparticle‐Based Artificial Transcription Factor

Advanced Functional Materials, EarlyView.
Oct4‐nanoscript, a biomimetic nanoparticle‐based artificial transcription factor, precisely regulates cellular rejuvenation by activating Oct4 target genes, restoring epigenetic marks, and reducing DNA damage. In a progeria model, it effectively rescued aging‐associated pathologies and extended lifespan.
Hongwon Kim, Junyeop Kim, Euiyeon Lee, Brandon Conklin, Yannan Hou, Sumin Kim, Yerim Hwang, Ki‐Bum Lee, Jongpil Kim   +8 more
wiley   +1 more source

ORIGINAL ARTICLE: Membrane Stabilizing Activity and Protein Denaturation: A Possible Mechanism of Action for the Anti-Inflammatory Activity of Phyllanthus amarus [PDF]

Journal of Krishna Institute of Medical Sciences University, 2012
Background: Previous studies confirm the anti-inflammatory potential of Phyllanthus amarus herb. Aim and objective: Primary aim of the present study was to investigate the possible anti-inflammatory mechanism of Phyllanthus amarus extract using in-vitro ...
Atul R. Chopade, Prakash M. Somade, Fahim J. Sayyad   +2 more
doaj  

Influence of Boiling, Steaming and Frying of Selected Leafy Vegetables on the In Vitro Anti-inflammation Associated Biological Activities

Plants, 2018
The aim of the present study was to evaluate the effect of cooking (boiling, steaming, and frying) on anti-inflammation associated properties in vitro of six popularly consumed green leafy vegetables in Sri Lanka, namely: Centella asiatica, Cassia ...
K. D. P. P. Gunathilake, K. K. D. S. Ranaweera, H. P. V. Rupasinghe   +2 more
doaj   +1 more source

The role of a disulfide bridge in the stability and folding kinetics of Arabidopsis thaliana cytochrome c6A [PDF]

, 2011
Cytochrome c 6A is a eukaryotic member of the Class I cytochrome c family possessing a high structural homology with photosynthetic cytochrome c 6 from cyanobacteria, but structurally and functionally distinct through the presence of a disulfide bond and
Arslan, Babul, Barker, Bartalesi, Beasty, Bertini, Betz, Bialek, Borgia, Brems, Carter, Chen, Chida, Christopher J. Howe, Cohen, Colon, Colon, Derek S. Bendall, Ellman, Elove, Feige, Gianni, Guidry, Gupta, Hogg, Howe, Jody M. Mason, Jonathan A.R. Worrall, Lange, Lange, Marcaida, Mason, Mason, Merchant, Mines, Molina-Heredia, Moore, Myers, Ogawa, Pace, Pace, Parrini, Pascher, Ptitsyn, Roder, Russell, Russell, Santoro, Satoh, Sauder, Schlarb-Ridley, Schmidt, Sosnick, Tezcan, Travaglini-Allocatelli, Travaglini-Allocatelli, Travaglini-Allocatelli, Ullah, Ullah, Wastl, Wastl, Wastl, Williams, Worrall, Worrall   +64 more
core   +1 more source

Engineering a Single Amino Acid Bionanozyme for Ultrasensitive Detection of Biomarkers: A WHO‐REASSURE‐ Aligned Approach

Advanced Functional Materials, EarlyView.
A unique 2D bionanozyme, engineered from a single amino acid and copper ions, demonstrates peroxidase‐mimicking catalytic activity. This efficient and simple bionanozyme allows for ultrasensitive, equipment‐free visual detection of key biomarkers in both test and real samples, meeting the WHO‐REASSURE standards for practical diagnostic applications ...
Subrat Vishwakarma, Om Shanker Tiwari, Vijay Kumar Patel, Ruchi Shukla, Ehud Gazit, Pandeeswar Makam   +5 more
wiley   +1 more source

Systematic FTIR Spectroscopy Study of the Secondary Structure Changes in Human Serum Albumin under Various Denaturation Conditions

Biomolecules, 2019
Human serum albumin (HSA) is the most abundant protein in blood plasma. HSA is involved in the transport of hormones, fatty acids, and some other compounds, maintenance of blood pH, osmotic pressure, and many other functions.
Dmitrii Usoltsev, Vera Sitnikova, Andrey Kajava, Mayya Uspenskaya   +3 more
doaj   +1 more source

Transmembrane Activation of Catalysis and Protein Refolding in Synthetic Cells by Enzymes and Nanozymes

Advanced Functional Materials, EarlyView.
Synthetic cells are engineered herein to respond to an external chemical messenger by the activation of intracellular catalysis. The chemical messenger molecules are catalytically generated by an extracellular enzyme or a mineral surface, whereas the intracellular catalysis emerges via direct enzyme activation or via protein refolding.
Dante G. Andersen, Andreas B. Pedersen, Mireia Casanovas Montasell, Søren Moesgaard, Anja Bille Bohn, Alexander N. Zelikin   +5 more
wiley   +1 more source