Results 11 to 20 of about 414,168 (349)

PRESSURE AND PROTEIN DENATURATION [PDF]

Journal of Biological Chemistry, 1946
Kinetic analyses have indicated that moderate hydrostatic pressures, up to some 700 atmospheres, oppose reversible and irreversible denaturations of certain enzyme systems, apparent at temperatures above the normal optimum of the enzyme reaction, as well as at lower temperatures in the presence of denaturants such as alcohol (1-4).
Johnson, Frank H., Campbell, Dan H.
openaire   +6 more sources

Cold and Warm Denaturation of Proteins

Journal of biological physics, 2001
We introduce a simplified protein model where the water degrees of freedom appear explicitly (although in an extremely simplified fashion). Using this model we are able to recover both the warm and the cold protein denaturation within a single framework, while addressing important issues about the structure of model proteins.
Caldarelli, G., Rios, P. De De
openaire   +7 more sources

Highly anomalous energetics of protein cold denaturation linked to folding-unfolding kinetics. [PDF]

PLoS ONE, 2011
Despite several careful experimental analyses, it is not yet clear whether protein cold-denaturation is just a "mirror image" of heat denaturation or whether it shows unique structural and energetic features. Here we report that, for a well-characterized
M Luisa Romero-Romero, Alvaro Inglés-Prieto, Beatriz Ibarra-Molero, Jose M Sanchez-Ruiz   +3 more
doaj   +1 more source

Irreversible denaturation of maltodextrin glucosidase studied by differential scanning calorimetry, circular dichroism, and turbidity measurements. [PDF]

PLoS ONE, 2014
Thermal denaturation of Escherichia coli maltodextrin glucosidase was studied by differential scanning calorimetry, circular dichroism (230 nm), and UV-absorption measurements (340 nm), which were respectively used to monitor heat absorption ...
Megha Goyal, Tapan K Chaudhuri, Kunihiro Kuwajima   +2 more
doaj   +1 more source

Effects of Whey Protein Denaturation and Whey Protein/Casein Ratio on the Stability and Whipping Properties of Recombined Cream [PDF]

Shipin Kexue, 2023
In this study, the effects of the denaturation degree of whey protein (WP) and whey protein/casein (WP/CN, m/m) ratio in milk powder on the emulsion stability and whipping properties of recombined cream were investigated.
LI Yue, XIE Yufei, LI Yang, LI Yan, ZHANG Liebing, YAN Jianguo
doaj   +1 more source

The regulatory subunit of PKA-I remains partially structured and undergoes β-aggregation upon thermal denaturation [PDF]

, 2011
Background: The regulatory subunit (R) of cAMP-dependent protein kinase (PKA) is a modular flexible protein that responds with large conformational changes to the binding of the effector cAMP.
A Horvath, A Natalello, A Zhou, AG Murzin, AM Arutyunyan, AM Fernandez-Escamilla, Angel L. Pey, Angela M. Gronenborn, Anja Underhaug Gjerde, AP Kornev, Aurora Martinez, C Kim, CE Poteet-Smith, CI Bayly, Claudine Mayer, D Hamada, D Vigil, DA Case, DA Leon, DA Leon, DT Haynie, E Deu, EL Greene, F Chiti, G Soldi, H Azakami, H LeVine 3rd, H Rehmann, I Luque, IJ Byeon, IM Plaza del Pino, In-Ja L. Byeon, J Sancho, J Wang, J Wang, J Wu, JA Beavo, JA Carney, JM Canaves, JM Louis, JM Sanchez-Ruiz, JP Ryckaert, JS Richardson, KE Tang, Khanh K. Dao, KK Dao, Knut Teigen, L Holm, M Fandrich, M Fandrich, M Manno, M Mauro, M Thórólfsson, MA Andrade, MS Akhtar, N Ferguson, N Kannan, P Banky, R Aoki, R Das, R Day, R Kopperud, R Shrivastava, RW Carrell, SO Doskeland, SS Taylor, SS Taylor, Stein O. Døskeland, TJ Kamerzell, U Essmann, V Vetri, VJ Hilser, W Wong, WD Cornell, WL Jorgensen, WN Lanzilotta, Y Su   +76 more
core   +11 more sources

Native protein denaturation using urea

BioTechniques, 2017
Protocol Summary Here we present a new protocol to analyze protein unfolding kinetics using a quantified real-time thermocycler. This technique enables the analysis of a wide range of denaturants (and their interactions with temperature change) on ...
Kyle K. Biggar, Neal J. Dawson, Kenneth B. Storey   +2 more
doaj   +1 more source

Non-Thermal Processing of a Protein Functional Beverage Using Pulsed Electric Fields: Escherichia coli Inactivation and Effect on Proteins

Beverages, 2022
The application of pulsed electric fields (PEFs) for the inactivation of Escherichia coli, suspended in a protein shake beverage and diluted with sterilized distilled water was carried out. Square bipolar pulses in the range of 25–40 kV/cm electric field
Mona Alzahrani, Filipa V. M. Silva, Sally Alkhafaji   +2 more
doaj   +1 more source

A DSC study of zinc binding to bovine serum albumin (BSA) [PDF]

Journal of the Serbian Chemical Society, 2007
The thermal denaturation of bovine serum albumin (BSA) is a kinetically and thermodynamically controlled process. The effects of zinc binding to bovine serum albumin (BSA), followed by differential scanning calorimetry (DSC), were investigated in this ...
SANJA OSTOJIC, VIDA DRAGUTINOVIC, MIODRAG KICANOVIC, BRANISLAV R. SIMONOVIC   +3 more
doaj   +3 more sources

Differential denaturation of serum proteome reveals a significant amount of hidden information in complex mixtures of proteins. [PDF]

PLoS ONE, 2013
UNLABELLED: Recently developed proteomic technologies allow to profile thousands of proteins within a high-throughput approach towards biomarker discovery, although results are not as satisfactory as expected.
Vincenzo Verdoliva, Cinzia Senatore, Maria Letizia Polci, Stefania Rossi, Martina Cordella, Giuseppe Carlucci, Paolo Marchetti, Giancarlo Antonini-Cappellini, Antonio Facchiano, Daniela D'Arcangelo, Francesco Facchiano   +10 more
doaj   +1 more source