Results 31 to 40 of about 5,755,949 (365)

Native protein denaturation using urea

open access: yesBioTechniques, 2017
Protocol Summary Here we present a new protocol to analyze protein unfolding kinetics using a quantified real-time thermocycler. This technique enables the analysis of a wide range of denaturants (and their interactions with temperature change) on ...
Kyle K. Biggar   +2 more
doaj   +1 more source

Differential denaturation of serum proteome reveals a significant amount of hidden information in complex mixtures of proteins. [PDF]

open access: yesPLoS ONE, 2013
UNLABELLED: Recently developed proteomic technologies allow to profile thousands of proteins within a high-throughput approach towards biomarker discovery, although results are not as satisfactory as expected.
Vincenzo Verdoliva   +10 more
doaj   +1 more source

Non-Thermal Processing of a Protein Functional Beverage Using Pulsed Electric Fields: Escherichia coli Inactivation and Effect on Proteins

open access: yesBeverages, 2022
The application of pulsed electric fields (PEFs) for the inactivation of Escherichia coli, suspended in a protein shake beverage and diluted with sterilized distilled water was carried out. Square bipolar pulses in the range of 25–40 kV/cm electric field
Mona Alzahrani   +2 more
doaj   +1 more source

Effects of Whey Protein Denaturation and Whey Protein/Casein Ratio on the Stability and Whipping Properties of Recombined Cream [PDF]

open access: yesShipin Kexue, 2023
In this study, the effects of the denaturation degree of whey protein (WP) and whey protein/casein (WP/CN, m/m) ratio in milk powder on the emulsion stability and whipping properties of recombined cream were investigated.
LI Yue, XIE Yufei, LI Yang, LI Yan, ZHANG Liebing, YAN Jianguo
doaj   +1 more source

POTENSI ANTIINFLAMASI JUS BUAH BELIMBING (Averrhoa carambola L.) TERHADAP DENATURASI PROTEIN IN VITRO

open access: yesBerkala Kedokteran, 2015
Starfruit (Averrhoa carambola L.) is an Asian tree that has been used in traditional medicine. Flavonoids, saponins, and tannins is thought to have antiinflammatory in inhibiting protein denaturation.
Farizka Erianti   +2 more
doaj   +1 more source

Effects of Wooden Breast Syndrome in Broiler Chicken on Sarcoplasmic, Myofibrillar, and Connective Tissue Proteins and Their Association with Muscle Fiber Area

open access: yesFoods, 2023
This study was conducted on chicken pectoralis major muscle with different wooden breast severity in combination with different sampling locations to investigate the effects of wooden breast syndrome on protein traits and total myofiber area, and their ...
Binbin Li   +3 more
doaj   +1 more source

Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients [PDF]

open access: yesElectrophoresis 18 (31/03/1997) 307-16, 2006
Membrane and nuclear proteins of poor solubility have been separated by high resolution two-dimensional (2-D) gel electrophoresis. Isoelectric focusing with immobilized pH gradients leads to severe quantitative losses of proteins in the resulting 2-D map, although the resolution is usually high.
arxiv   +1 more source

PHASE PROPERTIES OF PROTEIN SOLUTIONS AND PROTEIN DENATURATION

open access: yesTransactions of the Karelian Research Centre of the Russian Academy of Sciences, 2019
The formation of protein-containing condensed phases (crystals, oligomers, other solid aggregates, as well as dense liquids and gels), including membraneless micro and nanophases, plays an important role in the functional activity of proteins in the ...
Sergei Rozhkov, Andrei Goryunov
doaj   +1 more source

Thermal Characterisation and Isoconversional Kinetic Analysis of Osmotically Dried Pork Meat Proteins Longissimus dorsi

open access: yesFoods, 2023
The kinetic properties and thermal characteristics of fresh pork meat proteins (Longissimus dorsi), as well as osmotically dehydrated meat proteins, were investigated using differential scanning calorimetry.
Sanja Ostojić   +5 more
doaj   +1 more source

Denaturation studies on bovine serum albumin–bile salt system: Bile salt stabilizes bovine serum albumin through hydrophobicity

open access: yesJournal of Pharmaceutical Analysis, 2018
Protein denaturation is under intensive research, since it leads to neurological disorders of severe consequences. Avoiding denaturation and stabilizing the proteins in their native state is of great importance, especially when proteins are used as drug ...
Karpagaraj Malarkani   +2 more
doaj   +1 more source

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