Native protein denaturation using urea
BioTechniques, 2017 Protocol Summary Here we present a new protocol to analyze protein unfolding kinetics using a quantified real-time thermocycler. This technique enables the analysis of a wide range of denaturants (and their interactions with temperature change) on ...
Kyle K. Biggar +2 more
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Measurement of Heat and Pressure Induced Denaturation of Whey Protein Isolate Using Reversed-Phase HPLC and FTIR-Spectroscopy [PDF]
Trends in Peptide and Protein Sciences, 2019 The protein molecules experience various external stresses leading to denaturation of protein during the process of transforming original whey to the concentrated whey proteins or while the concentrated proteins are used in the protein-enriched food ...
Md. Amdadul Haque +3 more
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Studying Protein-Ligand Interactions by Protein Denaturation and Quantitative Cross-Linking Mass Spectrometry. [PDF]
Anal Chem, 2023 Recently, several mass spectrometry methods have utilized protein structural stability for the quantitative study of protein–ligand engagement. These protein-denaturation approaches, which include thermal proteome profiling (TPP) and stability of ...
Mathay M, Keller A, Bruce JE.
europepmc +2 more sources
Recipes for inducing cold denaturation in an otherwise stable protein. [PDF]
, 2022 Although cold denaturation is a fundamental phenomenon common to all proteins, it can only be observed in a handful of cases where it occurs at temperatures above the freezing point of water.
Giorgio Colombo (65952) +6 more
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Protein Denaturation Zero Entropy Temperature, and the Structure of Water Around Hydrophobic and Amphiphilic Solutes [PDF]
, 2020 The hydrophobic effect plays a key role in many chemical and biological processes, including protein folding. Nonetheless, a comprehensive picture of the effect of temperature on hydrophobic hydration and protein denaturation remains elusive.
Nuno, Galamba, kazimieras, Tamoliūnas
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The molecular basis of the effect of temperature on enzyme activity [PDF]
, 2010 Experimental data show that the effect of temperature on enzymes cannot be adequately explained in terms of a two-state model based on increases in activity and denaturation.
Oudshoorn, Matthew Leslie +17 more
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Berkala Kedokteran, 2015 Starfruit (Averrhoa carambola L.) is an Asian tree that has been used in traditional medicine. Flavonoids, saponins, and tannins is thought to have antiinflammatory in inhibiting protein denaturation.
Farizka Erianti +2 more
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Differential denaturation of serum proteome reveals a significant amount of hidden information in complex mixtures of proteins. [PDF]
PLoS ONE, 2013 UNLABELLED: Recently developed proteomic technologies allow to profile thousands of proteins within a high-throughput approach towards biomarker discovery, although results are not as satisfactory as expected.
Vincenzo Verdoliva +10 more
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Beverages, 2022 The application of pulsed electric fields (PEFs) for the inactivation of Escherichia coli, suspended in a protein shake beverage and diluted with sterilized distilled water was carried out. Square bipolar pulses in the range of 25–40 kV/cm electric field
Mona Alzahrani +2 more
doaj +1 more source
Single-molecule chemical denaturation of riboswitches [PDF]
, 2013 To date, single-molecule RNA science has been developed almost exclusively around the effect of metal ions as folding promoters and stabilizers of the RNA structure.
St-Pierre, Patrick +14 more
core +1 more source