Results 61 to 70 of about 16,988,925 (342)

Protein Dynamics and its Correlation to Protein Activity and Stability [PDF]

, 2009
The aim of this thesis is to investigate the role of fast protein dynamics (picosecond timescale) in enzyme activity and stability, and specifically to test the hypothesis that enzyme activity and stability are inversely related by their internal ...
Clement, David
core  

Efficient sampling of large-scale transition pathways and intermediate conformations in sub-mesoscopic protein complexes

Nature Communications
Protein conformational changes are the cornerstone of biological function. While conformers captured experimentally represent metastable states, the pathways connecting them have been elusive for experiments and simulations alike.
Domenico Scaramozzino, Byung Ho Lee, Laura Orellana   +2 more
doaj   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Predicting Protein Dynamics and Allostery Using Multi-Protein Atomic Distance Constraints

Structure, 2017
Summary The related concepts of protein dynamics, conformational ensembles and allostery are often difficult to study with molecular dynamics (MD) due to the timescales involved.
Joe G. Greener, Ioannis Filippis, M. Sternberg   +2 more
semanticscholar   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Sustained enzymatic activity and flow in crowded protein droplets

Nature Communications, 2021
Living cells can harvest environmental energy to drive chemical processes. Here the authors design a minimal artificial system that achieves steady states at similar metabolic densities to microorganisms.
Andrea Testa, Mirco Dindo, Aleksander A. Rebane, Babak Nasouri, Robert W. Style, Ramin Golestanian, Eric R. Dufresne, Paola Laurino   +7 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Neutron frequency windows and the protein dynamical transition [PDF]

, 2004
Proteins undergo an apparent dynamical transition on temperature variation that has been correlated with the onset of function. The transition in the mean-square displacement, ∆r2 , that is observed using a spectrometer or computer simulation, depends on
Becker, Torsten, Smith, Jeremy C., Finney, John L., Hayward, Jennifer A., Daniel, Roy M.   +4 more
core   +1 more source

How membranes shape up for lipid transfer

eLife
The extraction of a phospholipid called phosphatidic acid from the mitochondrial outer membrane is regulated by the curvature of this membrane.
Takashi Hirashima, Toshiya Endo
doaj   +1 more source

The absence of SOX2 in the anterior foregut alters the esophagus into trachea and bronchi in both epithelial and mesenchymal components

Biology Open, 2020
In the anterior foregut (AFG) of mouse embryos, the transcription factor SOX2 is expressed in the epithelia of the esophagus and proximal branches of respiratory organs comprising the trachea and bronchi, whereas NKX2.1 is expressed only in the epithelia
Machiko Teramoto, Ryo Sugawara, Katsura Minegishi, Masanori Uchikawa, Tatsuya Takemoto, Atsushi Kuroiwa, Yasuo Ishii, Hisato Kondoh   +7 more
doaj   +1 more source