Results 111 to 120 of about 7,816,364 (345)

Cellular and Secretory Proteins of the Salivary Glands of \u3cem\u3eSciara coprophila\u3c/em\u3e During the Larval-pupal Transformation [PDF]

, 1972
The cellular and secretory proteins of the salivary gland of Sciara coprophila during the stages of the larval-pupal transformation were examined by electrophoresis in 0.6 mm sheets of polyacrylamide gel with both SDS-continuous and discontinuous buffer ...
Been, Anita Cavagnaro, Rasch, Ellen M.
core   +1 more source

Analysis of cellular responses of macrophages to zinc ions and zinc oxide nanoparticles: a combined targeted and proteomic approach [PDF]

, 2014
Two different zinc oxide nanoparticles, as well as zinc ions, are used to study the cellular responses of the RAW 264 macrophage cell line. A proteomic screen is used to provide a wide view of the molecular effects of zinc, and the most prominent results
Armand, Lucie, Aude-Garcia, Catherine, Carrière, Marie, Collin-Faure, Véronique, Diemer, Hélène, Gerdil, Adèle, Habert, Aurélie, Hanau, Daniel, Herlin, Nathalie, Proamer, Fabienne, Rabilloud, Thierry, Strub, Jean-Marc, Triboulet, Sarah, Van Dorsselaer, Alain   +13 more
core   +4 more sources

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Electroblotting onto activated glass. High efficiency preparation of proteins from analytical sodium dodecyl sulfate-polyacrylamide gels for direct sequence analysis [PDF]

, 1986
We have developed a new method for the isolation of proteins for microsequencing. It consists of electrophoretic transfer (electroblotting) of proteins or their cleavage fragments onto activated glass filter paper sheets immediately after separation by ...
Aebersold, Ruedi H., Hood, Leroy E., Kent, Stephen B. H., Teplow, David B.   +3 more
core  

Molecular responses of mouse macrophages to copper and copper oxide nanoparticles inferred from proteomic analyses [PDF]

, 2013
The molecular responses of macrophages to copper-based nanoparticles have been investigated via a combination of proteomic and biochemical approaches, using the RAW264.7 cell line as a model.
Aude-Garcia, Catherine, Carrière, Marie, Chevallet, Mireille, Collin-Faure, Véronique, Diemer, Hélène, Habert, Aurélie, Hanau, Daniel, Herlin-Boime, Nathalie, Proamer, Fabienne, Rabilloud, Thierry, Strub, Jean-Marc, Triboulet, Sarah, Van Dorsselaer, Alain   +12 more
core   +5 more sources

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Journal of Biological Chemistry, 1977
A rapid and convenient method for peptide mapping of proteins has been developed. The technique, which is especially suitable for analysis of proteins that have been isolated from gels containg sodium dodecyl sulfate, involves partial enzymatic ...
D. Cleveland, S. Fischer, M. Kirschner, U. K. Laemmli   +3 more
semanticscholar   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Application of 2-D Electrophoresis for the Construction of “Protein Profiles” of Bacterial Cultures Lysates of Particularly Dangerous Infections Agents

Проблемы особо опасных инфекций, 2016
Objective of the study is to deploy 2-D electrophoresis for the construction of lysate “protein profiles” of bacterial cultures of particularly dangerous infections. Materials and methods.
T. A. Polunina, Yu. S. Varshavskaya, S. P. Zadnova, Ya. M. Krasnov   +3 more
doaj   +1 more source

Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.

Journal of Biological Chemistry, 1971
This report describes methods and results obtained by combining the techniques of sodium dodecyl sulfate (SDS) gel electrophoresis and electrophoresis in discontinuous buffer systems.
D. Neville
semanticscholar   +1 more source