Results 121 to 130 of about 7,816,364 (345)

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Characterisation of nicotine receptors on human peripheral blood mononuclear cells (PBMC) [PDF]

, 2009
“The original publication is available at www.springerlink.com”. Copyright Springer. DOI: 10.1007/s00011-008-8171-xAim and objective: The aim of the work was to characterise the nAChRs on human PBMC.
Parsons, M., Whelan, C.J., Wongsriraksa, A.   +2 more
core   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Utility of Nephlometry, ELISA and Serum Protein Electrophoresis as Diagnostic Tools for Alpha1-Antitrypsin Deficiency in COPD and Smokers

International Journal of Electrochemical Science, 2015
Evaluation of analytical techniques used in the detection of alpha1-antitrypsin (AAT) deficiency. The serum AAT was determined by several techniques, namely nephlometry, enzyme-linked immunosorbent assay (ELISA) and serum protein electrophoresis for 3 ...
Sree R. Isac, Noura Al-Jameil, Najat Hawsawi, Ahlam Buhairan, Rana Hassanato, Amina A. Hassan, Maha El-Tohamy   +6 more
doaj   +1 more source

Double-disc Electrophoresis of Proteins [PDF]

Nature, 1967
GEL electrophoresis in discontinuous buffer systems1 is a valuable method of examining dilute protein solutions. By selecting pH conditions it is possible to concentrate and then separate either cationic or anionic proteins, but only in separate analyses.
openaire   +2 more sources

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

Visual mnemonics for serum protein electrophoresis [PDF]

Medical Education Online, 2013
Carlos E. Medina-De la Garza, Marisela García-Hernández, María de los Ángeles Castro-Corona   +2 more
doaj   +1 more source

Standardized protocols for blood collection and analysis in elasmobranchs: a practical guide for clinicians and researchers

Frontiers in Veterinary Science
Blood analysis represents a crucial, yet often underutilized, diagnostic tool in elasmobranch medicine. Its broader application has been limited by the absence of standardized protocols and consistent species-specific reference values. The variability in
Hugo David, Daniel García-Párraga, Nuno Pereira, Pablo Morón-Elorza, Pablo Morón-Elorza   +4 more
doaj   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source