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False-Negative Urine Protein Electrophoresis by Semiautomated Gel Electrophoresis [PDF]
Urine is evaluated by electrophoretic methods both to gather information about the location and degree of damage within the nephron and to detect and quantify monoclonal free light chains (Bence Jones proteins). For many years, urine protein electrophoresis consisted of concentrating the urine (usually >50-fold) followed by manual application of the ...
David F, Keren +2 more
openaire +2 more sources
Protein electrophoresis is commonly used as an aid in the diagnosis of monoclonal gammopathies and is performed in many laboratories in Canada and throughout the world.
R. Booth +26 more
semanticscholar +1 more source
The guideline for western blotting assay
Western blotting is a technique for detecting protein developed based on protein electrophoresis separation and antigen‐antibody detection. It combines the high resolution of SDS polyacrylamide gel electrophoresis with the specificity of antigen‐antibody
Fang Wang +5 more
doaj +1 more source
Membrane proteins and proteomics: Love is possible, but so difficult [PDF]
Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with ...
Adessi +103 more
core +5 more sources
Sweet silver: A formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry [PDF]
Protein detection methods after electrophoresis have to be sensitive, homogeneous, and not to impair downstream analysis of proteins by MS. Speed, low cost, and user friendliness are also favored features. Silver staining combines many of these features,
Berggren +26 more
core +4 more sources
Objectives We investigated the frequency and pattern of detection of therapeutic monoclonal antibodies (t-mAbs) daratumumab and elotuzumab by routine serum protein electrophoresis (SPE) and immunofixation (IF) in treated patients with myeloma.
Felicia Tang +4 more
semanticscholar +1 more source
Identification of Structural Proteins of Rhizobium meliloti Temperate Phage 16-3 [PDF]
The structural proteins of Rhizobium meliloti temperate phage 16-3 have been analysed by means of polyacrylamide gel electrophoresis, isoelectric focusing and agarose gel electrophoresis.
Duda, Ernő +3 more
core +1 more source
Assessments of health variables in wild animal populations have evolved into important tools for characterizing spatiotemporal population trends and fitness, effects of stressors, diseases, and ecosystem health.
Nicole I. Stacy +2 more
doaj +1 more source
Monoclonal Gammopathy of Undetermined Significance with Three Bands on Serum Protein Electrophoresis: An Atypical Presentation [PDF]
Monoclonal Gammopathy (MGM) is a group of B-cell disorders characterised by secretion of abnormal monoclonal Immunoglobulin (Ig) that can be detected by presence of M-protein in serum and urine electrophoresis.
Rashmi Rasi Datta, Ashutosh Awasthi
doaj +1 more source
Isolation of Acidic, Basic and Neutral Metalloproteins by QPNC-PAGE [PDF]
A standard protocol for isolating metalloproteins in complex biological samples is presented using quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC ...
Bernd Kastenholz, David E. Garfin
core +2 more sources

