Results 241 to 250 of about 677,670 (306)

The Protein Engineering of Zearalenone Hydrolase Results in a Shift in the pH Optimum of the Relative Activity of the Enzyme. [PDF]

Toxins (Basel)
Dotsenko A, Sinelnikov I, Zorov I, Denisenko Y, Rozhkova A, Shcherbakova L.   +5 more
europepmc   +1 more source

Altering the mechanical properties of self-assembled filaments through engineering of EspA bacterial protein

Moran Elias-Mordechai, May Morhaim, Maya Georgia Pelah, Irina Rostovsky, May Nogaoker, Jürgen Jopp, Raz Zarivach, Neta Sal‐Man, Ronen Berkovich   +8 more
openalex   +1 more source

HSP70 governs permeability and mechanotransduction in primary human endothelial cells

FEBS Open Bio, EarlyView.
HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez, Everton G. Melo, Isadora C. B. Pavan, Percíllia V. S. Oliveira, Luiza B. C. T. Coimbra, Thaís L. S. Araujo   +5 more
wiley   +1 more source

Unifying sequence-structure coding for advanced protein engineering <i>via</i> a multimodal diffusion transformer. [PDF]

Chem Sci
Lin X, Chen Z, Li Y, Ma Z, Fan C, Cao Z, Feng S, Zhang J, Gao YQ.   +8 more
europepmc   +1 more source

Baculoviral Protein and DNA Co-delivery System for Mammalian Genome Engineering - raw Sanger sequencing and flow-cytometry data

Francesco Aulicino, Julien Capin, Alexandra Harrison, Renata A. Raele, Sathish K.N. Yadav, Dominique Baiwir, Gabriel Mazzucchelli, Loïc Quinton, Timothy J. Satchwell, Ashley M. Toye, Christiane Schaffitzel, Imre Berger   +11 more
openalex   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

New advances in protein engineering for industrial applications: Key takeaways. [PDF]

Open Life Sci
Ndochinwa GO, Wang QY, Okoro NO, Amadi OC, Nwagu TN, Nnamchi CI, Moneke AN, Odiba AS.   +7 more
europepmc   +1 more source

Editorial: Extremozymes: characteristics, structure, protein engineering and applications. [PDF]

Front Microbiol
Bhatt HB, Sani RK, Amoozegar MA, Singh SP.   +3 more
europepmc   +1 more source

Computational design of protein conformation for engineering enzyme specificity and promiscuity

Cavanagh, Peter Evan, Ting, Alice Y, Stanford University   +2 more
openalex   +1 more source