Results 21 to 30 of about 8,269,032 (242)

Protein engineering strategies for rational immunogen design

npj Vaccines, 2021
Antibody immunodominance refers to the preferential and asymmetric elicitation of antibodies against specific epitopes on a complex protein antigen.
T. Caradonna, A. Schmidt
semanticscholar   +1 more source

Application of fourier transform and proteochemometrics principles to protein engineering

BMC Bioinformatics, 2018
Background Connecting the dots between the protein sequence and its function is of fundamental interest for protein engineers. In-silico methods are useful in this quest especially when structural information is not available.
Frédéric Cadet, Nicolas Fontaine, Iyanar Vetrivel, Matthieu Ng Fuk Chong, Olivier Savriama, Xavier Cadet, Philippe Charton   +6 more
doaj   +1 more source

Publisher Correction: A machine learning approach for reliable prediction of amino acid interactions and its application in the directed evolution of enantioselective enzymes

Scientific Reports, 2021
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Frédéric Cadet, Nicolas Fontaine, Guangyue Li, Joaquin Sanchis, Matthieu Ng Fuk Chong, Rudy Pandjaitan, Iyanar Vetrivel, Bernard Offmann, Manfred T. Reetz   +8 more
doaj   +1 more source

A library approach for the de novo high-throughput isolation of humanized VHH domains with favorable developability properties following camelid immunization

mAbs, 2023
In this study, we generated a novel library approach for high throughput de novo identification of humanized single-domain antibodies following camelid immunization. To achieve this, VHH-derived complementarity-determining regions-3 (CDR3s) obtained from
Paul Arras, Han Byul Yoo, Lukas Pekar, Christian Schröter, Thomas Clarke, Simon Krah, Daniel Klewinghaus, Vanessa Siegmund, Andreas Evers, Stefan Zielonka   +9 more
doaj   +1 more source

An integrated mammalian library approach for optimization and enhanced microfluidics-assisted antibody hit discovery

Artificial Cells, Nanomedicine, and Biotechnology, 2023
Recent years have seen the development of a variety of mammalian library approaches for display and secretion mode. Advantages include library approaches for engineering, preservation of precious immune repertoires and their repeated interrogation, as ...
Ramona Gaa, Kavita Kumari, Hannah Melina Mayer, Desislava Yanakieva, Shang-Pu Tsai, Saurabh Joshi, Ralf Guenther, Achim Doerner   +7 more
doaj   +1 more source

Substrate multiplexed protein engineering facilitates promiscuous biocatalytic synthesis

Nature Communications, 2021
Enzymes with high activity are readily produced through protein engineering, but intentionally and efficiently engineering enzymes for an expanded substrate scope is a contemporary challenge.
Allwin D. McDonald, P. Higgins, A. Buller   +2 more
semanticscholar   +1 more source

Differential regulation of fibroblast growth factor receptor 1 trafficking and function by extracellular galectins

Cell Communication and Signaling, 2019
Fibroblast growth factor receptors (FGFRs) are integral membrane proteins that transmit signals through the plasma membrane. FGFRs signaling needs to be precisely adjusted as aberrant FGFRs function is associated with development of human cancers or ...
Marika Kucińska, Natalia Porębska, Agata Lampart, Marta Latko, Agata Knapik, Małgorzata Zakrzewska, Jacek Otlewski, Łukasz Opaliński   +7 more
doaj   +1 more source

A Novel T Cell-Engaging Bispecific Antibody for Treating Mesothelin-Positive Solid Tumors

Biomolecules, 2020
As mesothelin is overexpressed in various types of cancer, it is an attractive target for therapeutic antibodies. T-cell bispecific antibodies bind to target cells and engage T cells via binding to CD3, resulting in target cell killing by T-cell ...
Aerin Yoon, Shinai Lee, Sua Lee, Sojung Lim, Yong-Yea Park, Eunjung Song, Dong-Sik Kim, Kisu Kim, Yangmi Lim   +8 more
doaj   +1 more source

Cattle-derived knob paratopes grafted onto peripheral loops of the IgG1 Fc region enable the generation of a novel symmetric bispecific antibody format

Frontiers in Immunology, 2023
In this work we present a novel symmetric bispecific antibody format based on engraftments of cattle-derived knob paratopes onto peripheral loops of the IgG1 Fc region.
Desislava Yanakieva, Lena Vollmer, Andreas Evers, Vanessa Siegmund, Paul Arras, Lukas Pekar, Achim Doerner, Bernhard Valldorf, Harald Kolmar, Stefan Zielonka, Stefan Zielonka, Simon Krah   +11 more
doaj   +1 more source

Modular self-assembly system for development of oligomeric, highly internalizing and potent cytotoxic conjugates targeting fibroblast growth factor receptors

Journal of Biomedical Science, 2021
Background Overexpression of FGFR1 is observed in numerous tumors and therefore this receptor constitutes an attractive molecular target for selective cancer treatment with cytotoxic conjugates.
Marta Poźniak, Natalia Porębska, Kamil Jastrzębski, Mateusz Adam Krzyścik, Marika Kucińska, Weronika Zarzycka, Agnieszka Barbach, Małgorzata Zakrzewska, Jacek Otlewski, Marta Miączyńska, Łukasz Opaliński   +10 more
doaj   +1 more source