Results 41 to 50 of about 8,576,413 (316)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Engineering of a femtomolar affinity binding protein to human serum albumin

, 2008
We describe the development of a novel serum albumin binding protein showing an extremely high affinity (K(D)) for HSA in the femtomolar range. Using a naturally occurring 46-residue three-helix bundle albumin binding domain (ABD) of nanomolar affinity ...
Harne, Nina, Abrahmsén, Lars, Nygren, Per-Åke,, Jonsson, Andreas,, Dogan, Jakob,   +4 more
core   +1 more source

Determining biophysical protein stability in lysates by a fast proteolysis assay, FASTpp. [PDF]

, 2012
The biophysical stability is an important parameter for protein activity both in vivo and in vitro. Here we propose a method to analyse thermal melting of protein domains in lysates: Fast parallel proteolysis (FASTpp).
Rüdiger, Stefan G. D., Stefan G D Rüdiger, Maurice, Madelon M., David P Minde, Sub Cellular Protein Chemistry, Stefan G. D. Rüdiger, Madelon M Maurice, Minde, David P., Cellular Protein Chemistry, David P. Minde, Madelon M. Maurice   +10 more
core   +3 more sources

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Antibody-induced dimerization of FGFR1 promotes receptor endocytosis independently of its kinase activity

Scientific Reports, 2017
Fibroblast growth factors (FGFs) and their plasma membrane-localized receptors (FGFRs) play a key role in the regulation of developmental processes and metabolism.
Łukasz Opaliński, Aleksandra Sokołowska-Wędzina, Martyna Szczepara, Małgorzata Zakrzewska, Jacek Otlewski   +4 more
doaj   +1 more source

Protein disulfide engineering

FEBS Letters, 2013
Improving the stability of proteins is an important goal in many biomedical and industrial applications. A logical approach is to emulate stabilizing molecular interactions found in nature. Disulfide bonds are covalent interactions that provide substantial stability to many proteins and conform to well‐defined geometric conformations, thus making them ...
Dombkowski, Alan A., Sultana, Kazi Zakia, Craig, Douglas B.   +2 more
openaire   +2 more sources

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Phosphoglycerate dehydrogenase promotes pancreatic cancer development by interacting with eIF4A1 and eIF4E

Journal of Experimental & Clinical Cancer Research, 2019
Background Pancreatic cancer is one of the most malignant cancers. The overall 5-year survival rate of its patients is 8%, the lowest among major cancer types.
Xuhui Ma, Boya Li, Jie Liu, Yan Fu, Yongzhang Luo   +4 more
doaj   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

UniSePT: an engineered broadly applicable Unique Secretory signal PepTide for high-yield biotherapeutic production in mammalian cell-based expression systems

Journal of Biological Engineering
Background The yield of therapeutic proteins in mammalian cell-based production systems is often limited by inefficient secretion and reduced bioactivity caused by incompatible secretory signal peptides (SPs).
Goutam Ulgekar, Satarupa Dutta, Ealisha Jain, Meenakshi Mansukhani, Harshada Thawari, Subeer S. Majumdar, Nirmalya Ganguli   +6 more
doaj   +1 more source