Results 41 to 50 of about 1,300,384 (288)

Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi. [PDF]

, 2015
Gene knockout strategies, RNAi and rescue experiments are all employed to study mammalian gene function. However, the disadvantages of these approaches include: loss of function adaptation, reduced viability and gene overexpression that rarely matches ...
Dowdy, Steven F, Kaulich, Manuel, Lee, Yeon J, Lönn, Peter, Meade, Bryan R, Springer, Aaron D   +5 more
core   +1 more source

Engineering Nutritious Proteins [PDF]

Biotechnology and Genetic Engineering Reviews, 1997
(1997). Engineering Nutritious Proteins. Biotechnology and Genetic Engineering Reviews: Vol. 14, No. 1, pp. 191-210.
openaire   +2 more sources

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

The intracellular interplay between galectin-1 and FGF12 in the assembly of ribosome biogenesis complex

Cell Communication and Signaling
Galectins constitute a class of lectins that specifically interact with β-galactoside sugars in glycoconjugates and are implicated in diverse cellular processes, including transport, autophagy or signaling. Since most of the activity of galectins depends
Aleksandra Gędaj, Aleksandra Chorążewska, Krzysztof Ciura, Radosław Karelus, Dominika Żukowska, Martyna Biaduń, Marta Kalka, Małgorzata Zakrzewska, Natalia Porębska, Łukasz Opaliński   +9 more
doaj   +1 more source

Protein-based materials, toward a new level of structural control [PDF]

, 2001
Through billions of years of evolution nature has created and refined structural proteins for a wide variety of specific purposes. Amino acid sequences and their associated folding patterns combine to create elastic, rigid or tough materials.
Tirrell, David A., van Hest, Jan C. M.
core   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Antibody-induced dimerization of FGFR1 promotes receptor endocytosis independently of its kinase activity

Scientific Reports, 2017
Fibroblast growth factors (FGFs) and their plasma membrane-localized receptors (FGFRs) play a key role in the regulation of developmental processes and metabolism.
Łukasz Opaliński, Aleksandra Sokołowska-Wędzina, Martyna Szczepara, Małgorzata Zakrzewska, Jacek Otlewski   +4 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Phosphoglycerate dehydrogenase promotes pancreatic cancer development by interacting with eIF4A1 and eIF4E

Journal of Experimental & Clinical Cancer Research, 2019
Background Pancreatic cancer is one of the most malignant cancers. The overall 5-year survival rate of its patients is 8%, the lowest among major cancer types.
Xuhui Ma, Boya Li, Jie Liu, Yan Fu, Yongzhang Luo   +4 more
doaj   +1 more source

Beyond bispecificity: Controlled Fab arm exchange for the generation of antibodies with multiple specificities

mAbs, 2022
Controlled Fab arm exchange (cFAE) has proven to be a generic and versatile technology for the efficient generation of IgG-like bispecific antibodies (DuoBodies or DBs), with several in clinical development and one product, amivantamab, approved by the ...
Desislava Yanakieva, Lukas Pekar, Andreas Evers, Markus Fleischer, Stephan Keller, Dirk Mueller-Pompalla, Lars Toleikis, Harald Kolmar, Stefan Zielonka, Simon Krah   +9 more
doaj   +1 more source