Results 21 to 30 of about 1,300,384 (288)

Improved split fluorescent proteins for endogenous protein labeling. [PDF]

, 2017
Self-complementing split fluorescent proteins (FPs) have been widely used for protein labeling, visualization of subcellular protein localization, and detection of cell-cell contact.
Feng, Siyu, Huang, Bo, Leonetti, Manuel D, Li, Han, Pessino, Veronica, Sekine, Sayaka   +5 more
core   +2 more sources

A Novel T Cell-Engaging Bispecific Antibody for Treating Mesothelin-Positive Solid Tumors

Biomolecules, 2020
As mesothelin is overexpressed in various types of cancer, it is an attractive target for therapeutic antibodies. T-cell bispecific antibodies bind to target cells and engage T cells via binding to CD3, resulting in target cell killing by T-cell ...
Aerin Yoon, Shinai Lee, Sua Lee, Sojung Lim, Yong-Yea Park, Eunjung Song, Dong-Sik Kim, Kisu Kim, Yangmi Lim   +8 more
doaj   +1 more source

Modular self-assembly system for development of oligomeric, highly internalizing and potent cytotoxic conjugates targeting fibroblast growth factor receptors

Journal of Biomedical Science, 2021
Background Overexpression of FGFR1 is observed in numerous tumors and therefore this receptor constitutes an attractive molecular target for selective cancer treatment with cytotoxic conjugates.
Marta Poźniak, Natalia Porębska, Kamil Jastrzębski, Mateusz Adam Krzyścik, Marika Kucińska, Weronika Zarzycka, Agnieszka Barbach, Małgorzata Zakrzewska, Jacek Otlewski, Marta Miączyńska, Łukasz Opaliński   +10 more
doaj   +1 more source

Detection of intermediates and kinetic control during assembly of bacteriophage P22 procapsid [PDF]

, 2008
Bacteriophage P22 serves as a model for the assembly and maturation of other icosahedral double-stranded DNA viruses. P22 coat and scaffolding proteins assemble in vitro into an icosahedral procapsid, which then expands during DNA packaging (maturation).
Abendon, Canady, Casjens, Ceres, Earnshaw, Endres, Falkner, Feigin, Fowler, Galisteo, Golub, Guinier, Hendrix, Hiro Tsuruta, Jiang, Kang, Kenneth H. French, Kihara, King, Lanman, Paatero, Paatero, Parent, Parent, Parent, Parker, Parker, Parker, Parker, Parker, Peter E. Prevelige, Prasad, Prevelige, Prevelige, Prevelige, Quinn, Reddy, Roman Tuma, Sakalian, Stockley, Stray, Sun, Svergun, Thuman-Commike, Thuman-Commike, Thuman-Commike, Tuma, Wang, Wang, Wyckoff, Zhang, Zlotnick, Zlotnick, Zlotnick   +53 more
core   +1 more source

A coarse-grained protein model in a water-like solvent [PDF]

, 2013
Simulations employing an explicit atom description of proteins in solvent can be computationally expensive. On the other hand, coarse-grained protein models in implicit solvent miss essential features of the hydrophobic effect, especially its temperature
Buldyrev, Sergey V., Debenedetti, Pablo G., Kumar, Sanat K., Rossky, Peter J., Sharma, Sumit, Stanley, Harry Eugene   +5 more
core   +1 more source

Cattle-derived knob paratopes grafted onto peripheral loops of the IgG1 Fc region enable the generation of a novel symmetric bispecific antibody format

Frontiers in Immunology, 2023
In this work we present a novel symmetric bispecific antibody format based on engraftments of cattle-derived knob paratopes onto peripheral loops of the IgG1 Fc region.
Desislava Yanakieva, Lena Vollmer, Andreas Evers, Vanessa Siegmund, Paul Arras, Lukas Pekar, Achim Doerner, Bernhard Valldorf, Harald Kolmar, Stefan Zielonka, Stefan Zielonka, Simon Krah   +11 more
doaj   +1 more source

Comparison of protein quantification and extraction methods suitable for E-coli cultures [PDF]

, 2008
Many different extraction and analysis methods exist to determine the protein fraction of microbial cells. For metabolic engineering purposes it is important to have precise and accurate measurements.
De Mey, Marjan, De Muynck, Cassandra, Lequeux, Gaspard, Maertens, Jo, Soetaert, Wim, Vandamme, Erick   +5 more
core   +2 more sources

Solid-phase synthesis of protein-polymers on reversible immobilization supports

Nature Communications, 2018
Synthesis of protein-polymer conjugates typically relies on multi-step processes in solution and on challenging purification strategies. Here the authors show a robust synthesis approach which eliminates purification processes by immobilizing proteins ...
Hironobu Murata, Sheiliza Carmali, Stefanie L. Baker, Krzysztof Matyjaszewski, Alan J. Russell   +4 more
doaj   +1 more source

Versatile and rapid microfluidics-assisted antibody discovery

mAbs, 2021
Recent years have seen unparalleled development of microfluidic applications for antibody discovery in both academic and pharmaceutical research. Microfluidics can support native chain-paired library generation as well as direct screening of antibody ...
Ramona Gaa, Emmanuel Menang-Ndi, Shruti Pratapa, Christine Nguyen, Satyendra Kumar, Achim Doerner   +5 more
doaj   +1 more source

Structural and dynamic changes associated with beneficial engineered single-amino-acid deletion mutations in enhanced green fluorescent protein. [PDF]

, 2014
Single-amino-acid deletions are a common part of the natural evolutionary landscape but are rarely sampled during protein engineering owing to limited and prejudiced molecular understanding of mutations that shorten the protein backbone.
Arpino, JA, Jones, DD, Rizkallah, PJ
core   +3 more sources