Results 21 to 30 of about 8,576,413 (316)

A Novel T Cell-Engaging Bispecific Antibody for Treating Mesothelin-Positive Solid Tumors

Biomolecules, 2020
As mesothelin is overexpressed in various types of cancer, it is an attractive target for therapeutic antibodies. T-cell bispecific antibodies bind to target cells and engage T cells via binding to CD3, resulting in target cell killing by T-cell ...
Aerin Yoon, Shinai Lee, Sua Lee, Sojung Lim, Yong-Yea Park, Eunjung Song, Dong-Sik Kim, Kisu Kim, Yangmi Lim   +8 more
doaj   +1 more source

Modular self-assembly system for development of oligomeric, highly internalizing and potent cytotoxic conjugates targeting fibroblast growth factor receptors

Journal of Biomedical Science, 2021
Background Overexpression of FGFR1 is observed in numerous tumors and therefore this receptor constitutes an attractive molecular target for selective cancer treatment with cytotoxic conjugates.
Marta Poźniak, Natalia Porębska, Kamil Jastrzębski, Mateusz Adam Krzyścik, Marika Kucińska, Weronika Zarzycka, Agnieszka Barbach, Małgorzata Zakrzewska, Jacek Otlewski, Marta Miączyńska, Łukasz Opaliński   +10 more
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CHAPTER 1.1. Disulfide Bonds in Protein Folding and Stability [PDF]

, 2018
Disulfide bonds are unique among post-translational modifications, as they add covalent crosslinks to the polypeptide chain. Accordingly, they can exert pronounced effects on protein folding and stability. This is of particular importance for secreted or
Sub Cellular Protein Chemistry, Hendershot, Linda M., Feige, Matthias J., Ineke Braakman, Matthias J. Feige, Braakman, Ineke, Cellular Protein Chemistry, Linda M. Hendershot   +7 more
core   +1 more source

Cattle-derived knob paratopes grafted onto peripheral loops of the IgG1 Fc region enable the generation of a novel symmetric bispecific antibody format

Frontiers in Immunology, 2023
In this work we present a novel symmetric bispecific antibody format based on engraftments of cattle-derived knob paratopes onto peripheral loops of the IgG1 Fc region.
Desislava Yanakieva, Lena Vollmer, Andreas Evers, Vanessa Siegmund, Paul Arras, Lukas Pekar, Achim Doerner, Bernhard Valldorf, Harald Kolmar, Stefan Zielonka, Stefan Zielonka, Simon Krah   +11 more
doaj   +1 more source

Solid-phase synthesis of protein-polymers on reversible immobilization supports

Nature Communications, 2018
Synthesis of protein-polymer conjugates typically relies on multi-step processes in solution and on challenging purification strategies. Here the authors show a robust synthesis approach which eliminates purification processes by immobilizing proteins ...
Hironobu Murata, Sheiliza Carmali, Stefanie L. Baker, Krzysztof Matyjaszewski, Alan J. Russell   +4 more
doaj   +1 more source

Versatile and rapid microfluidics-assisted antibody discovery

mAbs, 2021
Recent years have seen unparalleled development of microfluidic applications for antibody discovery in both academic and pharmaceutical research. Microfluidics can support native chain-paired library generation as well as direct screening of antibody ...
Ramona Gaa, Emmanuel Menang-Ndi, Shruti Pratapa, Christine Nguyen, Satyendra Kumar, Achim Doerner   +5 more
doaj   +1 more source

Improvement of downstream processing of recombinant proteins by means of genetic engineering [PDF]

, 1993
Flaschel E, Friehs K. Improvement of downstream processing of recombinant proteins by means of genetic engineering. Biotechnology Advances. 1993;11(1):31-77.The rapid advancement of genetic engineering has allowed to produce an impressive number of ...
Flaschel, Erwin, Friehs, Karl
core   +1 more source

Engineering with NanoLuc: A playground for the development of bioluminescent protein switches and sensors

, 2020
The small engineered luciferase NanoLuc has rapidly become a powerful tool in the fields of biochemistry, chemical biology, and cell biology due to its exceptional brightness and stability.
Rosier, Bas J.H.M., Biewenga, Lieuwe, Merkx, Maarten   +2 more
core   +1 more source

Development of a high yielding expression platform for the introduction of non-natural amino acids in protein sequences

mAbs, 2020
The ability to genetically encode non-natural amino acids (nnAAs) into proteins offers an expanded tool set for protein engineering. nnAAs containing unique functional moieties have enabled the study of post-translational modifications, protein ...
Gargi Roy, Jason Reier, Andrew Garcia, Tom Martin, Megan Rice, Jihong Wang, Meagan Prophet, Ronald Christie, William Dall’Acqua, Sanjeev Ahuja, Michael A Bowen, Marcello Marelli   +11 more
doaj   +1 more source

Molecular mechanism of CCDC106 regulating the p53-Mdm2/MdmX signaling axis

Scientific Reports, 2023
The tumor suppressor p53 (p53) is regulated by murine double minute 2 (Mdm2) and its homologous MdmX in maintaining the basal level of p53. Overexpressed Mdm2/MdmX inhibits cellular p53 activity, which is highly relevant to cancer occurrence. Coiled-coil
Ting Zhou, Zhiqiang Ke, Qianqian Ma, Jiani Xiang, Meng Gao, Yongqi Huang, Xiyao Cheng, Zhengding Su   +7 more
doaj   +1 more source