Results 31 to 40 of about 765,059 (313)

Engineering with NanoLuc: A playground for the development of bioluminescent protein switches and sensors

, 2020
The small engineered luciferase NanoLuc has rapidly become a powerful tool in the fields of biochemistry, chemical biology, and cell biology due to its exceptional brightness and stability.
Rosier, Bas J.H.M., Biewenga, Lieuwe, Merkx, Maarten   +2 more
core   +1 more source

Engineering protein switches : sensors, regulators, and spare parts for biology and biotechnology

, 2011
Switch protein switch! Proteins that switch between distinct conformational states are ideal for monitoring and controlling molecular processes in biological systems.
Vinkenborg, J.L., Merkx, M., Koay, M.S.T., Melissa S. Koay, Golynskiy, M.V., Jan L. Vinkenborg, Misha V. Golynskiy, Maarten Merkx   +7 more
core   +1 more source

A novel one-step approach for the construction of yeast surface display Fab antibody libraries

Microbial Cell Factories, 2018
Background Yeast surface display (YSD) has proven to be a versatile platform technology for antibody discovery. However, the construction of antibody Fab libraries typically is a tedious three-step process that involves the generation of heavy chain as ...
Simon Rosowski, Stefan Becker, Lars Toleikis, Bernhard Valldorf, Julius Grzeschik, Deniz Demir, Iris Willenbücher, Ramona Gaa, Harald Kolmar, Stefan Zielonka, Simon Krah   +10 more
doaj   +1 more source

Leveraging the multivalent p53 peptide-MdmX interaction to guide the improvement of small molecule inhibitors

Nature Communications, 2022
Peptide fragments derived from the interfaces of protein-protein interactions (PPIs) provide useful templates for designing small molecule PPI inhibitors.
Xiyao Cheng, Rong Chen, Ting Zhou, Bailing Zhang, Zichun Li, Meng Gao, Yongqi Huang, Huili Liu, Zhengding Su   +8 more
doaj   +1 more source

Genetic engineering for protein engineering.

Nihon Kessho Gakkaishi, 1987
Recent development of genetic recombination technique for study of the function-structure relationships of protein is reviewed. After elucidation of the nature of genetic message, fundamentals in the technique, both creating recombinant DNA molecules and the isolation of cloned genes are explained and some of the in vitro mutagenesis are mentioned for ...
MORIYAMA, Hideaki, OKADA, Hirosuke
openaire   +2 more sources

Expressed protein ligation for a large dimeric protein

, 2011
Expressed protein ligation (EPL) is a protein engineering tool for post-translational ligation of protein or peptide fragments. This technique allows modification of specific parts of proteins, opening possibilities for incorporating probes for ...
NMR Spectroscopy, Karagöz, G.E., Sub Cellular Protein Chemistry, O. Hsieh, Sub NMR Spectroscopy, T. Sinnige, Rüdiger, S.G.D., Hsieh, O., Sinnige, T, G. E. Karagoz, S. G. D. Rudiger, Cellular Protein Chemistry   +11 more
core   +1 more source

A Bibliometric Analysis of Publications in Uremic Toxins From 1991 to 2024

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Background Uremic toxins are a growing area of research in nephrology, with significant implications in the progression and treatment of chronic kidney disease (CKD) and the management of end‐stage kidney disease (ESKD). This bibliometric analysis aims to evaluate the global research trends, key contributors, and the impact of publications in ...
Yuh‐Shan Ho, Karim M. Soliman, Wisit Cheungpasitporn, Wannasit Wathanavasin, Ahmed Daoud, Ahmed I. Kamal, Balázs Szamosfalvi, Tibor Fülöp   +7 more
wiley   +1 more source

DNA‐Mediated Protein Shuttling between Coacervate‐Based Artificial Cells [PDF]

, 2022
The regulation of protein uptake and secretion is crucial for (inter)cellular signaling. Mimicking these molecular events is essential when engineering synthetic cellular systems.
Bas J. H. M. Rosier, Hirayama, Shota, Cornelissens, Femke R.A., Takashi Hayashi, Tsuyoshi Mashima, Alexander F. Mason, Brunsveld, Luc, Rosier, Bas J.H.M., Altenburg, Wiggert J., Oohora, Koji, Femke R. A. Cornelissens, Jan C. M. van Hest, van Stevendaal, Marleen H.M.E., Koji Oohora, Wiggert J. Altenburg, Brunsveld, Luc; id_orcid, Mashima, Tsuyoshi, van Hest, Jan C. M., Shota Hirayama, Marleen H. M. E. van Stevendaal, Cornelissens, Femke R. A., van Hest, Jan C.M., Hayashi, Takashi, Luc Brunsveld, Mason, Alexander F., Rosier, Bas J. H. M., van Stevendaal, Marleen H. M. E.   +26 more
core   +1 more source

Protein engineering strategies to optimise recombinant product synthesis and accumulation in Nicotiana benthamiana. [PDF]

Plant Biotechnol J
In plant molecular farming, recombinant products include traditional protein targets, such as antibodies, antigens, and enzymes, but also increasingly refers to high-value peptides, and small molecules produced by heterologous enzymatic pathways.
Harding MD, Sainsbury F.
europepmc   +2 more sources

Experience With Performing Rheocarna Therapy via the Single‐Needle Method for Treatment of Chronic Limb‐Threatening Ischemia

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Introduction This study investigated the safety and efficacy of single‐needle Rheocarna therapy for chronic limb‐threatening ischemia (CLTI) with wounds. Methods Six patients with CLTI involving ulcers unresponsive to revascularization underwent single‐needle Rheocarna treatment.
Yasutaka Yamauchi, Rie Tanaka, Reona Kawahira, Shingo Nabeta, Kouhei Naoi, Kazuhito Kitajima, Akira Wada, Tomonari Takagi, Norihiko Ohura, Akira Miyamoto   +9 more
wiley   +1 more source