Results 221 to 230 of about 434,956 (254)

Mapping Protein–Protein Interactions by Quantitative Proteomics

2010
Proteins exert their function inside a cell generally in multiprotein complexes. These complexes are highly dynamic structures changing their composition over time and cell state. The same protein may thereby fulfill different functions depending on its binding partners.
Dengjel, Jörn, Kratchmarova, Irina, Blagoev, Blagoy   +2 more
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Mapping of RNA–protein interactions

Analytica Chimica Acta, 2009
RNA-protein interactions are important biological events that perform multiple functions in all living organisms. The wide range of RNA interactions demands diverse conformations to provide contacts for the selective recognition of proteins. Various analytical procedures are presently available for quantitative analyses of RNA-protein complexes, but ...
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Mapping protein–protein interactions by mass spectrometry

Current Opinion in Biotechnology, 2006
Mass spectrometry is currently at the forefront of technologies for mapping protein-protein interactions, as it is a highly sensitive technique that enables the rapid identification of proteins from a variety of biological samples. When used in combination with affinity purification and/or chemical cross-linking, whole or targeted protein interaction ...
Julian, Vasilescu, Daniel, Figeys
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Protein‐Protein Interaction Mapping by 2C‐BioID

Current Protocols in Cell Biology, 2019
AbstractProtein‐protein interactions (PPIs) add an essential layer of complexity to the information encoded by the genome. Modulation of such interactions is a key feature of most, if not all, cellular activities and allows cells to respond rapidly to both internal and external signals and stimuli. In this respect, the development of the BioID assay to
Alexandre, Chojnowski, Hendrikje, Werner, Matthew, Cook, Radoslaw M, Sobota, Brian, Burke, Colin L, Stewart   +5 more
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Novel approaches to map protein interactions

Current Opinion in Biotechnology, 2003
Although we now have the sequence of the human genome at hand, we face the challenge of assigning function to the identified genes. Genes usually ascribe their function through proteins, and the role of proteins is to interact with other molecules. Therefore, if we could map the interactions of proteins we would be able to understand protein function ...
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The protein–protein interaction map of Helicobacter pylori

Nature, 2001
With the availability of complete DNA sequences for many prokaryotic and eukaryotic genomes, and soon for the human genome itself, it is important to develop reliable proteome-wide approaches for a better understanding of protein function. As elementary constituents of cellular protein complexes and pathways, protein-protein interactions are key ...
J C, Rain, L, Selig, H, De Reuse, V, Battaglia, C, Reverdy, S, Simon, G, Lenzen, F, Petel, J, Wojcik, V, Schächter, Y, Chemama, A, Labigne, P, Legrain   +12 more
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Progress and potential of Drosophila protein interaction maps

Pharmacogenomics, 2000
Protein-protein interactions mediate many important cellular processes and are central to the mechanisms by which most proteins function. Charting the interactions among the proteins involved in a process has been an essential step in characterising the function of proteins and pathways.

exaly   +3 more sources

Deciphering complex protein interaction kinetics using Interaction Map

Biochemical and Biophysical Research Communications, 2012
Cellular receptor systems are expected to present complex ligand interaction patterns that cannot be evaluated assuming a simple one ligand:one receptor interaction model. We have previously evaluated heterogeneous interactions using an alternative method to regression analysis, called Interaction Map (IM).
Altschuh, D, Bjorkelund, H, Strandgard, J, Choulier, Laurence, Malmqvist, M, Andersson, K   +5 more
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Mapping protein–protein interactions with combinatorial biology methods

Current Opinion in Biotechnology, 2001
Extremely diverse, DNA-encoded libraries of peptides and proteins have been constructed that include a linkage between each polypeptide and the encoding DNA. Library members can be selected by virtue of a particular binding specificity, and their protein sequence can be deduced from the sequence of the cognate DNA.
J, Pelletier, S, Sidhu
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Proteolytic DNA for Mapping Protein−DNA Interactions

Biochemistry, 2002
We describe a technique to determine sites on proteins involved in protein-DNA interactions. DNA was synthesized via polymerase chain reaction (PCR) to produce four polynucleotide products with phosphorothioate nucleotides at the A, T, G, or C residues.
Brian D, Schmidt, Claude F, Meares
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