Fundamentals of Protein Interaction Network Mapping.
Molecular Systems Biology, 2015 Studying protein interaction networks of all proteins in an organism (“interactomes”) remains one of the major challenges in modern biomedicine. Such information is crucial to understanding cellular pathways and developing effective therapies for the ...
Zhong Yao, Max Kotlyar, Igor Jurišica
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Systematic protein–protein interaction mapping for clinically relevant human GPCRs [PDF]
Molecular Systems Biology, 2017 G‐protein‐coupled receptors (GPCRs) are the largest family of integral membrane receptors with key roles in regulating signaling pathways targeted by therapeutics, but are difficult to study using existing proteomics technologies due to their complex ...
Kate Sokolina +27 more
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Author Correction: Systematic protein-protein interaction mapping for clinically relevant human GPCRs [PDF]
Molecular Systems BiologyKate Sokolina +27 more
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Mapping of domain-mediated protein-protein interaction by SPOT peptide assay
STAR Protocols, 2021 Summary: Identification of peptides mediating protein-protein interaction (PPI) is crucial for understanding the function of interlinked proteins in cellular processes and amino acid-associated diseases.
Xiaoyi Mao +4 more
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A Protein Interaction Map of the Mitotic Spindle [PDF]
Molecular Biology of the Cell, 2007 The mitotic spindle consists of a complex network of proteins that segregates chromosomes in eukaryotes. To strengthen our understanding of the molecular composition, organization, and regulation of the mitotic spindle, we performed a system-wide two-hybrid screen on 94 proteins implicated in spindle function in Saccharomyces cerevisiae. We report 604
Wong, Jonathan +11 more
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Mapping protein–DNA interactions with DiMeLo-seq [PDF]
Nature Protocols, 2022 Abstract We recently developed Di rected Me thylation with Lo ng-read seq uencing (DiMeLo-seq) to map protein-DNA interactions genome wide.
Annie Maslan +8 more
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Mapping of protein-protein interaction sites in the plant-type [2Fe-2S] ferredoxin. [PDF]
PLoS ONE, 2011 Knowing the manner of protein-protein interactions is vital for understanding biological events. The plant-type [2Fe-2S] ferredoxin (Fd), a well-known small iron-sulfur protein with low redox potential, partitions electrons to a variety of Fd-dependent ...
Haruka Kameda +3 more
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Reconstructing genome-wide protein-protein interaction networks using multiple strategies with homologous mapping. [PDF]
PLoS ONE, 2015 BACKGROUND:One of the crucial steps toward understanding the biological functions of a cellular system is to investigate protein-protein interaction (PPI) networks.
Yu-Shu Lo +4 more
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The protein interaction map of bacteriophage lambda [PDF]
BMC Microbiology, 2011 Bacteriophage lambda is a model phage for most other dsDNA phages and has been studied for over 60 years. Although it is probably the best-characterized phage there are still about 20 poorly understood open reading frames in its 48-kb genome. For a complete understanding we need to know all interactions among its proteins.
Rajagopala, Seesandra V. +2 more
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Detecting Mutually Exclusive Interactions in Protein-Protein Interaction Maps
PLoS ONE, 2012 Comprehensive protein interaction maps can complement genetic and biochemical experiments and allow the formulation of new hypotheses to be tested in the system of interest. The computational analysis of the maps may help to focus on interesting cases and thereby to appropriately prioritize the validation experiments.
Carmen Sanchez Claros, TRAMONTANO, ANNA
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