Systematic protein–protein interaction mapping for clinically relevant human GPCRs [PDF]
G‐protein‐coupled receptors (GPCRs) are the largest family of integral membrane receptors with key roles in regulating signaling pathways targeted by therapeutics, but are difficult to study using existing proteomics technologies due to their complex ...
Kate Sokolina +27 more
doaj +2 more sources
The MAPPIT (mammalian protein protein interaction trap) method allows high-throughput detection of protein interactions by very simple co-transfection of three plasmids in HEK293T cells, followed by a luciferase readout. MAPPIT detects a large percentage
Delphine Masschaele +2 more
exaly +2 more sources
Protein–protein interaction site mapping using NMR-detected mutational scanning
We demonstrate a novel NMR method for the mapping of protein-protein interaction sites. In our approach protein-protein binding sites are mapped by competition binding experiments using indirect NMR reporter technology and Ala positional scanning.
Georg Kontaxis +2 more
exaly +2 more sources
A simple, static contact mapping algorithm has been developed as a first step at identifying potential peptide biomimetics from protein interaction partner structure files.
Michael H Peters
exaly +3 more sources
Author Correction: Systematic protein-protein interaction mapping for clinically relevant human GPCRs [PDF]
Kate Sokolina +27 more
doaj +2 more sources
Protein Interaction Mapping Identifies RBBP6 as a Negative Regulator of Ebola Virus Replication [PDF]
Jyoti Batra +2 more
exaly +2 more sources
Comparative Flavivirus-Host Protein Interaction Mapping Reveals Mechanisms of Dengue and Zika Virus Pathogenesis [PDF]
Priya S Shāh +2 more
exaly +2 more sources
Mapping of domain-mediated protein-protein interaction by SPOT peptide assay
Summary: Identification of peptides mediating protein-protein interaction (PPI) is crucial for understanding the function of interlinked proteins in cellular processes and amino acid-associated diseases.
Xiaoyi Mao +4 more
doaj +1 more source
Mapping of protein-protein interaction sites in the plant-type [2Fe-2S] ferredoxin. [PDF]
Knowing the manner of protein-protein interactions is vital for understanding biological events. The plant-type [2Fe-2S] ferredoxin (Fd), a well-known small iron-sulfur protein with low redox potential, partitions electrons to a variety of Fd-dependent ...
Haruka Kameda +3 more
doaj +1 more source
Reconstructing genome-wide protein-protein interaction networks using multiple strategies with homologous mapping. [PDF]
BACKGROUND:One of the crucial steps toward understanding the biological functions of a cellular system is to investigate protein-protein interaction (PPI) networks.
Yu-Shu Lo +4 more
doaj +1 more source

