Results 21 to 30 of about 267,218 (263)
A Yeast BiFC-seq Method for Genome-wide Interactome Mapping
Genomics, Proteomics & Bioinformatics, 2022 Genome-wide physical protein–protein interaction (PPI) mapping remains a major challenge for current technologies. Here, we reported a high-efficiency BiFC-seq method, yeast-enhanced green fluorescent protein-based bimolecular fluorescence ...
Limin Shang +11 more
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Protein-protein interaction map for yeast TFIID [PDF]
Nucleic Acids Research, 2003 A major rate-limiting step in transcription initiation by RNA polymerase II is recognition and binding of the TATA element by the transcription factor TFIID. TFIID is composed of TATA binding protein (TBP) and approximately a dozen TBP-associated factors (TAFs).
Gayatri, Yatherajam +3 more
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Pan-cancer mapping of differential protein-protein interactions [PDF]
Scientific Reports, 2020 AbstractDeciphering the variations in the protein interactome is required to reach a systems-level understanding of tumorigenesis. To accomplish this task, we have considered the clinical and transcriptome data on >6000 samples from The Cancer Genome Atlas for 12 different cancers.
Gulfidan, Gizem +4 more
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eLife, 2016 To improve chemical cross-linking of proteins coupled with mass spectrometry (CXMS), we developed a lysine-targeted enrichable cross-linker containing a biotin tag for affinity purification, a chemical cleavage site to separate cross-linked peptides away
Dan Tan +20 more
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A Partitioned Approach to Protein Interaction Mapping [PDF]
, 2002 This is a brief description of a new program for drawing protein interactions in three-dimensional space. Our program divides nodes into three groups: biconnected subgraph in the center, terminal nodes at the outermost region, and the rest in between them.
Yanga Byun, Euna Jeong, Kyungsook Han
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Mass spectrometry‐based protein–protein interaction networks for the study of human diseases
Molecular Systems Biology, 2021 A better understanding of the molecular mechanisms underlying disease is key for expediting the development of novel therapeutic interventions. Disease mechanisms are often mediated by interactions between proteins. Insights into the physical rewiring of
Alicia L Richards +2 more
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Mapping oncogenic protein interactions for precision medicine [PDF]
International Journal of Cancer, 2022 AbstractNormal protein‐protein interactions (normPPIs) occur with high fidelity to regulate almost every physiological process. In cancer, this highly organised and precisely regulated network is disrupted, hijacked or reprogrammed resulting in oncogenic protein‐protein interactions (oncoPPIs). OncoPPIs, which can result from genomic alterations, are a
Mehdi Sharifi Tabar +4 more
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Nature Communications, 2016 Mapping protein-protein interaction using crosslinking and mass spectroscopy strategies is hampered by a high rate of false-positive results. Here, the authors develop a genetically encoded photo-affinity probe for accurate identification of protein ...
Yi Yang +8 more
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iRefIndex: A consolidated protein interaction database with provenance
BMC Bioinformatics, 2008 Background Interaction data for a given protein may be spread across multiple databases. We set out to create a unifying index that would facilitate searching for these data and that would group together redundant interaction data while recording the ...
Magklaras George +2 more
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Determining protein complex connectivity using a probabilistic deletion network derived from quantitative proteomics. [PDF]
PLoS ONE, 2009 Protein complexes are key molecular machines executing a variety of essential cellular processes. Despite the availability of genome-wide protein-protein interaction studies, determining the connectivity between proteins within a complex remains a major ...
Mihaela E Sardiu +6 more
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