Results 21 to 30 of about 292,859 (260)
A Yeast BiFC-seq Method for Genome-wide Interactome Mapping
Genomics, Proteomics & Bioinformatics, 2022 Genome-wide physical protein–protein interaction (PPI) mapping remains a major challenge for current technologies. Here, we reported a high-efficiency BiFC-seq method, yeast-enhanced green fluorescent protein-based bimolecular fluorescence ...
Limin Shang +11 more
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eLife, 2016 To improve chemical cross-linking of proteins coupled with mass spectrometry (CXMS), we developed a lysine-targeted enrichable cross-linker containing a biotin tag for affinity purification, a chemical cleavage site to separate cross-linked peptides away
Dan Tan +20 more
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A Partitioned Approach to Protein Interaction Mapping [PDF]
, 2002 This is a brief description of a new program for drawing protein interactions in three-dimensional space. Our program divides nodes into three groups: biconnected subgraph in the center, terminal nodes at the outermost region, and the rest in between them.
Yanga Byun, Euna Jeong, Kyungsook Han
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Drosophila Protein interaction Map (DPiM) [PDF]
Fly, 2012 Proteins perform essential cellular functions as part of protein complexes, often in conjunction with RNA, DNA, metabolites and other small molecules. The genome encodes thousands of proteins but not all of them are expressed in every cell type; and expressed proteins are not active at all times.
Guruharsha, K.G. +6 more
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Mapping oncogenic protein interactions for precision medicine [PDF]
International Journal of Cancer, 2022 Abstract Normal protein‐protein interactions (normPPIs) occur with high fidelity to regulate almost every physiological process. In cancer, this highly organised and precisely regulated network is disrupted, hijacked or reprogrammed resulting in oncogenic protein‐protein interactions (oncoPPIs). OncoPPIs, which can result from genomic
Mehdi Sharifi Tabar +4 more
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Nature Communications, 2016 Mapping protein-protein interaction using crosslinking and mass spectroscopy strategies is hampered by a high rate of false-positive results. Here, the authors develop a genetically encoded photo-affinity probe for accurate identification of protein ...
Yi Yang +8 more
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iRefIndex: A consolidated protein interaction database with provenance
BMC Bioinformatics, 2008 Background Interaction data for a given protein may be spread across multiple databases. We set out to create a unifying index that would facilitate searching for these data and that would group together redundant interaction data while recording the ...
Magklaras George +2 more
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Mass spectrometry‐based protein–protein interaction networks for the study of human diseases
Molecular Systems Biology, 2021 A better understanding of the molecular mechanisms underlying disease is key for expediting the development of novel therapeutic interventions. Disease mechanisms are often mediated by interactions between proteins. Insights into the physical rewiring of
Alicia L Richards +2 more
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Determining protein complex connectivity using a probabilistic deletion network derived from quantitative proteomics. [PDF]
PLoS ONE, 2009 Protein complexes are key molecular machines executing a variety of essential cellular processes. Despite the availability of genome-wide protein-protein interaction studies, determining the connectivity between proteins within a complex remains a major ...
Mihaela E Sardiu +6 more
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Conservation of binding properties in protein models
Computational and Structural Biotechnology Journal, 2021 We study the models submitted to round 12 of the Critical Assessment of protein Structure Prediction (CASP) experiment to assess how well the binding properties are conserved when the X-ray structures of the target proteins are replaced by their models ...
Megan Egbert +7 more
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