Results 1 to 10 of about 1,408,289 (218)

An optimized protocol for acquiring and processing cryo-EM data of human 26S proteasome with M1-Ub6

STAR Protocols, 2021
Summary: The 26S proteasome is specialized for regulated protein degradation. It is formed by a regulatory particle (RP) that recognizes ubiquitinated substrates and caps a hollow cylindrical core particle (CP) where substrates are proteolyzed ...
Xiang Chen, Dan Shi, Ping Zhang, Kylie J. Walters   +3 more
doaj   +1 more source

The Kinase Specificity of Protein Kinase Inhibitor Peptide

Frontiers in Pharmacology, 2021
G-protein-coupled-receptor (GPCR) signaling is exquisitely controlled to achieve spatial and temporal specificity. The endogenous protein kinase inhibitor peptide (PKI) confines the spatial and temporal spread of the activity of protein kinase A (PKA ...
Yao Chen, Yao Chen, Bernardo L. Sabatini
doaj   +1 more source

Ca2+/calmodulin-dependent protein kinase kinase is not involved in hypothalamic AMP-activated protein kinase activation by neuroglucopenia. [PDF]

PLoS ONE, 2012
Hypoglycemia and neuroglucopenia stimulate AMP-activated protein kinase (AMPK) activity in the hypothalamus and this plays an important role in the counterregulatory responses, i.e.
Junji Kawashima, Thierry Alquier, Youki Tsuji, Odile D Peroni, Barbara B Kahn   +4 more
doaj   +1 more source

Proteomic and Phosphoproteomic Profiling Reveals the Oncogenic Role of Protein Kinase D Family Kinases in Cholangiocarcinoma

Cells, 2022
Cholangiocarcinoma (CCA) is a lethal malignancy in the hepatobiliary system, with dysregulated protein expression and phosphorylation signaling. However, the protein and phosphorylation signatures of CCAs are little-known.
Yun Lu, Xiangyu Li, Kai Zhao, Yuanxin Shi, Zhengdong Deng, Wei Yao, Jianming Wang   +6 more
doaj   +1 more source

FGIN-1-27 Inhibits Melanogenesis by Regulating Protein Kinase A/cAMP-Responsive Element-Binding, Protein Kinase C-β, and Mitogen-Activated Protein Kinase Pathways

Frontiers in Pharmacology, 2020
FGIN-1-27 is a synthetic mitochondrial diazepam binding inhibitor receptor (MDR) agonist that has demonstrated pro-apoptotic, anti-anxiety, and steroidogenic activity in various studies.
Jinpeng Lv, Songzhou Jiang, Ying Yang, Ximei Zhang, Rongyin Gao, Yan Cao, Guoqiang Song   +6 more
doaj   +1 more source

Chemical genetic identification of GAK substrates reveals its role in regulating Na + /K + -ATPase

Life Science Alliance, 2018
Novel GAK phosphorylation targets are identified using chemical genetic methods. One of the substrates is the α subunit of the Na + /K + -ATPase, phosphorylation of which is necessary for its surface trafficking from endosomes. Conserved functions of NAK
Amy W Lin, Kalbinder K Gill, Marisol Sampedro Castañeda, Irene Matucci, Noreen Eder, Suzanne Claxton, Helen Flynn, Ambrosius P Snijders, Roger George, Sila K Ultanir   +9 more
doaj   +1 more source

Knockout of the PKN Family of Rho Effector Kinases Reveals a Non-redundant Role for PKN2 in Developmental Mesoderm Expansion

Cell Reports, 2016
In animals, the protein kinase C (PKC) family has expanded into diversely regulated subgroups, including the Rho family-responsive PKN kinases. Here, we describe knockouts of all three mouse PKN isoforms and reveal that PKN2 loss results in lethality at ...
Ivan Quétier, Jacqueline J.T. Marshall, Bradley Spencer-Dene, Sylvie Lachmann, Adele Casamassima, Claudio Franco, Sarah Escuin, Joseph T. Worrall, Priththivika Baskaran, Vinothini Rajeeve, Michael Howell, Andrew J. Copp, Gordon Stamp, Ian Rosewell, Pedro Cutillas, Holger Gerhardt, Peter J. Parker, Angus J.M. Cameron   +17 more
doaj   +1 more source

Calcium/calmodulin-dependent protein kinase kinase 2 regulates hepatic fuel metabolism

Molecular Metabolism, 2022
Objective: The liver is the primary internal metabolic organ that coordinates whole body energy homeostasis in response to feeding and fasting. Genetic ablation or pharmacological inhibition of calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2)
Brittany A. Stork, Adam Dean, Andrea R. Ortiz, Pradip Saha, Nagireddy Putluri, Maricarmen D. Planas-Silva, Iqbal Mahmud, Kimal Rajapakshe, Cristian Coarfa, Stefan Knapp, Philip L. Lorenzi, Bruce E. Kemp, Benjamin E. Turk, John W. Scott, Anthony R. Means, Brian York   +15 more
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Mitochondrial protein import under kinase surveillance

Microbial Cell, 2014
Despite the simplicity of the yeast Saccharomyces cerevisiae,its basic cellular machinery tremendously mirrors that of higher eukaryotic counterparts.
Magdalena Opalińska, Chris Meisinger
doaj   +1 more source

Protein Kinase Inhibitor Peptide as a Tool to Specifically Inhibit Protein Kinase A

Frontiers in Physiology, 2020
The protein kinase enzyme family plays a pivotal role in almost every aspect of cellular function, including cellular metabolism, division, proliferation, transcription, movement, and survival.
Chong Liu, Ping Ke, Jingjing Zhang, Xiaoying Zhang, Xiongwen Chen   +4 more
doaj   +1 more source