Crystal Structures Reveal Hidden Domain Mechanics in Protein Kinase A (PKA) [PDF]
Biology, 2023 Cyclic-AMP-dependent protein kinase A (PKA) is a critical enzyme involved in various signaling pathways that plays a crucial role in regulating cellular processes including metabolism, gene transcription, cell proliferation, and differentiation.
Colin L. Welsh +2 more
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Phosphorylation of protein kinase A (PKA) regulatory subunit RIα by protein kinase G (PKG) primes PKA for catalytic activity in cells [PDF]
Journal of Biological Chemistry, 2018 cAMP-dependent protein kinase (PKAc) is a pivotal signaling protein in eukaryotic cells. PKAc has two well-characterized regulatory subunit proteins, RI and RII (each having α and β isoforms), which keep the PKAc catalytic subunit in a catalytically inactive state until activation by cAMP.
Hemal Patel +2 more
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Characterizing protein kinase A (PKA) subunits as macromolecular regulators of PKA RIα liquid-liquid phase separation. [PDF]
J Chem Phys, 2021 Researchers developed a method called fast Fourier transform (FFT)-based modeling of atomistic protein–protein interactions applied to cross second virial coefficient B23(FMAPB23) to characterize protein kinase A (PKA) subunits as macromolecular regulators of PKA RIα liquid-liquid phase separation. The cross second virial coefficient B23 was calculated
Ahn SH +5 more
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Functional Insights into Protein Kinase A (PKA) Signaling from C. elegans. [PDF]
Life (Basel), 2022 Protein kinase A (PKA), which regulates a diverse set of biological functions downstream of cyclic AMP (cAMP), is a tetramer consisting of two catalytic subunits (PKA-C) and two regulatory subunits (PKA-R). When cAMP binds the PKA-R subunits, the PKA-C subunits are released and interact with downstream effectors. In Caenorhabditis elegans (C. elegans),
Sadeghian F +3 more
europepmc +4 more sources
Evolution of the cAMP-dependent protein kinase (PKA) catalytic subunit isoforms. [PDF]
PLoS ONE, 2017 The 3',5'-cyclic adenosine monophosphate (cAMP)-dependent protein kinase, or protein kinase A (PKA), pathway is one of the most versatile and best studied signaling pathways in eukaryotic cells.
Kristoffer Søberg +3 more
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Protein Implicated in Nonsyndromic Mental Retardation Regulates Protein Kinase A (PKA) Activity [PDF]
Journal of Biological Chemistry, 2012 Mutation of the coiled-coil and C2 domain-containing 1A (CC2D1A) gene, which encodes a C2 domain and DM14 domain-containing protein, has been linked to severe autosomal recessive nonsyndromic mental retardation. Using a mouse model that produces a truncated form of CC2D1A that lacks the C2 domain and three of the four DM14 domains, we show that CC2D1A ...
Jun Qin, Bing Su, Sung Yun Jung
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cAMP-dependent Protein Kinase (PKA) Signaling Is Impaired in the Diabetic Heart [PDF]
Journal of Biological Chemistry, 2015 Diabetes mellitus causes cardiac dysfunction and heart failure that is associated with metabolic abnormalities and autonomic impairment. Autonomic control of ventricular function occurs through regulation of cAMP-dependent protein kinase (PKA). The diabetic heart has suppressed β-adrenergic responsiveness, partly attributable to receptor changes, yet ...
Kenneth Humphries
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The allosteric mechanism induced by protein kinase A (PKA) phosphorylation of dematin (band 4.9). [PDF]
J Biol Chem, 2015 Dematin (band 4.9) is an F-actin binding and bundling protein best known for its role within red blood cells, where it both stabilizes as well as attaches the spectrin/actin cytoskeleton to the erythrocytic membrane. Here, we investigate the structural consequences of phosphorylating serine 381, a covalent modification that turns off F-actin bundling ...
Chen L +4 more
europepmc +6 more sources
cAMP-dependent activation of the Rac guanine exchange factor P-REX1 by type I protein kinase A (PKA) regulatory subunits [PDF]
Journal of Biological Chemistry, 2019 JOSÉ Vázquez-Prado +2 more
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The Kinase Specificity of Protein Kinase Inhibitor Peptide
Frontiers in Pharmacology, 2021 G-protein-coupled-receptor (GPCR) signaling is exquisitely controlled to achieve spatial and temporal specificity. The endogenous protein kinase inhibitor peptide (PKI) confines the spatial and temporal spread of the activity of protein kinase A (PKA ...
Yao Chen, Yao Chen, Bernardo L. Sabatini
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