T Cell Exhaustion in Cancer Immunotherapy: Heterogeneity, Mechanisms, and Therapeutic Opportunities
Advanced Science, EarlyView.T cell exhaustion limits immunotherapy efficacy. This article delineates its progression from stem‐like to terminally exhausted states, governed by persistent antigen, transcription factors, epigenetics, and metabolism. It maps the exhaustion landscape in the TME and proposes integrated reversal strategies, providing a translational roadmap to overcome
Yang Yu +7 more
wiley +1 more source
An in vitro Assay to Screen for Substrates of PKA Using Phospho-motif-specific Antibodies
Bio-Protocol, 2020 Kinases function as regulators of many cellular processes such as cell migration. These enzymes typically phosphorylate target motif sequences. Mass spec or phospho-specific antibody detection can be used to determine whether a kinase can phosphorylate ...
David Gau, Partha Roy
doaj +1 more source
Phosphorylation of Spinophilin Modulates Its Interaction with Actin Filaments [PDF]
, 2003 Spinophilin is a protein phosphatase 1 (PP1)- and actin-binding protein that modulates excitatory synaptic transmission and dendritic spine morphology. We report that spinophilin is phosphorylated in vitro by protein kinase A (PKA).
Allen, Patrick B. +5 more
core
The Enigmatic Canal-Associated Neurons Regulate Caenorhabditis elegans Larval Development Through a cAMP Signaling Pathway. [PDF]
, 2019 Caenorhabditis elegans larval development requires the function of the two Canal-Associated Neurons (CANs): killing the CANs by laser microsurgery or disrupting their development by mutating the gene ceh-10 results in early larval arrest. How these cells
Chien, Jason +5 more
core +1 more source
Studies of mice with cyclic AMP-dependent protein kinase (PKA) defects reveal the critical role of PKA’s catalytic subunits in anxiety [PDF]
Behavioural Brain Research, 2016 Cyclic adenosine mono-phosphate-dependent protein kinase (PKA) is critically involved in the regulation of behavioral responses. Previous studies showed that PKA's main regulatory subunit, R1α, is involved in anxiety-like behaviors. The purpose of this study was to determine how the catalytic subunit, Cα, might affect R1α's function and determine its ...
George, Briassoulis +9 more
openaire +2 more sources
Advanced Science, EarlyView.This research identifies DTX3L as a critical tumor suppressor that inhibits epithelial‐mesenchymal transition (EMT), invasion and metastasis in gastric cancer. By functioning as an E3 ubiquitin ligase, DTX3L targets the master EMT regulator SNAI1 for GSK‐3β‐dependent proteasomal degradation.
Yang Chen +7 more
wiley +1 more source
Advanced Science, EarlyView.This study identifies a FOSL2‐driven positive feedback loop that amplifies FSH/FSHR signaling. During FSH‐dependent follicle maturation, FSH induces Fosl2 expression via the cAMP‐PKA‐CREB cascade. FOSL2 in turn binds the promoters of Fshr and estrogen‐biosynthesis genes to enhance their transcription, thereby increasing Fshr mRNA level and amplifying ...
Hongru Shi +13 more
wiley +1 more source
Specification of spatial relationships in directed graphs of cell signaling networks [PDF]
, 2008 Graph theory provides a useful and powerful tool for the analysis of cellular signaling networks. Intracellular components such as cytoplasmic signaling proteins, transcription factors and genes are connected by links, representing various types of ...
Azi Lipshtat +2 more
core +1 more source
Decoding Undesirable Inflammatory Responses of Nucleic Acid‐Delivering Lipid Nanoparticles
Advanced Science, EarlyView.Lipid nanoparticles (LNPs) enable efficient nucleic acid delivery, but their immunogenicity is a double‐edged sword. This review explores LNP‐driven innate and adaptive immunity, covering lipid components, endosomal escape, and nucleic acid sensing.
Ruimin Hu +6 more
wiley +1 more source
Enhanced Integrin α4β1-Mediated Adhesion Contributes to a Mobilization Defect of Endothelial Progenitor Cells in Diabetes. [PDF]
, 2016 Diabetes is associated with a deficit of circulating endothelial progenitor cells (EPCs), which has been attributed to their defective mobilization from the bone marrow.
Abplanalp, Wesley T +6 more
core +2 more sources