Results 41 to 50 of about 10,358,150 (287)

Human growth hormone inclusion bodies present native-like secondary and tertiary structures which can be preserved by mild solubilization for refolding

Microbial Cell Factories, 2022
Background Native-like secondary structures and biological activity have been described for proteins in inclusion bodies (IBs). Tertiary structure analysis, however, is hampered due to the necessity of mild solubilization conditions.
Rosa Maria Chura-Chambi, Chuck Shaker Farah, Ligia Morganti   +2 more
doaj   +1 more source

A systematic structural comparison of all solved small proteins deposited in PDB. The effect of disulfide bonds in protein fold

Computational and Structural Biotechnology Journal, 2021
Defensins are small proteins, usually ranging from 3 to 6 kDa, amphipathic, disulfide-rich, and with a small or even absent hydrophobic core. Since a hydrophobic core is generally found in globular proteins that fold in an aqueous solvent, the peculiar ...
Mariana H. Moreira, Fabio C.L. Almeida, Tatiana Domitrovic, Fernando L. Palhano   +3 more
doaj   +1 more source

Bioinformatics analysis of LPD06283 protein of Rhodococcus opacus

Siliao yanjiu, 2023
The study was to investigate the structure and function of LPD06283 protein of Rhodococcus opacus PD630. Bioinformatics methods were used to analyze the physicochemical properties, hydrophilicity and hydrophobicity, amphiphilic α helix, homology, signal ...
MA Xue-jing, BAI Jing, HAN Jing-xuan, BAI Xiao-meng, HAN Ran, LI Bo-ying, HAO Ya-ru, ZHANG Zhao-ying   +7 more
doaj  

Crystal structure of domain of unknown function 507 (DUF507) reveals a new protein fold

Scientific Reports, 2023
The crystal structure of the domain of unknown function family 507 protein from Aquifex aeolicus is reported (AaDUF507, UniProt O67633, 183 residues). The structure was determined in two space groups (C2221 and P3221) at 1.9 Å resolution.
Cole E. McKay, Jianlin Cheng, John J. Tanner   +2 more
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Bioinformatics analysis of microalgal lipid droplet proteins

Siliao yanjiu, 2023
The experiment was to explore the structural features of microalgal lipid droplet proteins. Bioinformatics methods were used to investigate the physicochemical properties, hydrophilicity/hydrophobicity, signal peptide, transmembrane domain, amphipathic α-
MA Xue-jing, LIANG Meng-meng, CONG Hui, LIANG Yan-zhong, ZHANG Zheng-kai, GUO Zi-yue, WANG Jing-lin, ZHANG Zhao-ying   +7 more
doaj  

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Multiple co-evolutionary networks are supported by the common tertiary scaffold of the LacI/GalR proteins. [PDF]

PLoS ONE, 2013
Protein families might evolve paralogous functions on their common tertiary scaffold in two ways. First, the locations of functionally-important sites might be "hard-wired" into the structure, with novel functions evolved by altering the amino acid (e.g.
Daniel J Parente, Liskin Swint-Kruse
doaj   +1 more source