Results 31 to 40 of about 1,326,151 (303)
We developed a new method to analyze protein–protein interactions using a dual-inducible prokaryotic expression system. To evaluate protein–protein binding, a chimeric fusion toxin gene was constructed using a DNase-treated short DNA fragment (epitope ...
Agnieszka M. Murakami +5 more
doaj +1 more source
Two RNA-binding motifs in eIF3 direct HCV IRES-dependent translation. [PDF]
The initiation of protein synthesis plays an essential regulatory role in human biology. At the center of the initiation pathway, the 13-subunit eukaryotic translation initiation factor 3 (eIF3) controls access of other initiation factors and mRNA to the
Arias-Palomo, Ernesto +6 more
core +1 more source
Cholesterol down-regulates BK channels stably expressed in HEK 293 cells. [PDF]
Cholesterol is one of the major lipid components of the plasma membrane in mammalian cells and is involved in the regulation of a number of ion channels.
Wei Wu +4 more
doaj +1 more source
Tetrodotoxin (TTX)-binding proteins are present in toxic TTX-bearing animals, such as pufferfish and gastropods. These may prevent autotoxicity. However, TTX-binding proteins are also found in the nontoxic marine shore crab, Hemigrapsus sanguineus. Here,
Yuji Nagashima +5 more
doaj +1 more source
Biogenesis of mitochondrial ubiquinol [PDF]
The precursor proteins to the subunits of ubiquinol:cytochrome c reductase (cytochrome bc1 complex) of Neurospora crassa were synthesized in a reticulocyte lysate.
Neupert, Walter +3 more
core +1 more source
Analytical Insights into Protein–Alum Interactions and Their Impact on Conformational Epitope
Several alum-adjuvanted vaccines have been licensed in the past 40 years. Despite its extensive and continuous use, the immune mechanism of action of alum adjuvants is not yet completely understood. Many different variables during the formulation process
Alessio Corrado +7 more
doaj +1 more source
An atypical porcine pestivirus (APPV) causing congenital tremor type A-II in piglets was identified in China in 2016. An increased number of cases of APPV have been reported in various countries all over the world since 2015.
Huawei Zhang +5 more
doaj +1 more source
Transcription-translation coupling: direct interactions of RNA polymerase with ribosomes and ribosomal subunits. [PDF]
In prokaryotes, RNA polymerase and ribosomes can bind concurrently to the same RNA transcript, leading to the functional coupling of transcription and translation.
Blaha, Gregor M +9 more
core +1 more source
Protein Kinase A Regulatory Subunits in Colon Cancer
The protein kinase A (PKA) is classified as type I or II depending on the association of the catalytic subunit with either the RI or RII regulatory subunits.
Chris C. Carlson +4 more
doaj +1 more source
Single-peptide DNA-dependent RNA polymerase homologous to multi-subunit RNA polymerase [PDF]
Transcription in all living organisms is accomplished by multi-subunit RNA polymerases (msRNAPs). msRNAPs are highly conserved in evolution and invariably share a B400 kDa five-subunit catalytic core.
Forrest, David +3 more
core +2 more sources

