How to change the oligomeric state of a circular protein assembly: switch from 11-subunit to 12-subunit TRAP suggests a general mechanism. [PDF]
PLoS ONE, 2011 Many critical cellular functions are performed by multisubunit circular protein oligomers whose internal geometry has evolved to meet functional requirements.
Chao-Sheng Chen +6 more
doaj +1 more source
Interaction between the assembly of the ribosomal subunits: Disruption of 40S ribosomal assembly causes accumulation of extra-ribosomal 60S ribosomal protein uL18/L5. [PDF]
PLoS ONE, 2020 Inhibition of the synthesis of an essential ribosomal protein (r-protein) abrogates the assembly of its cognate subunit, while assembly of the other subunit continues.
Nusrat Rahman +2 more
doaj +1 more source
Cholesterol down-regulates BK channels stably expressed in HEK 293 cells. [PDF]
PLoS ONE, 2013 Cholesterol is one of the major lipid components of the plasma membrane in mammalian cells and is involved in the regulation of a number of ion channels.
Wei Wu +4 more
doaj +1 more source
Breaking tolerance in transgenic mice expressing the human TSH receptor A-subunit: thyroiditis, epitope spreading and adjuvant as a 'double edged sword'. [PDF]
PLoS ONE, 2012 Transgenic mice with the human thyrotropin-receptor (TSHR) A-subunit targeted to the thyroid are tolerant of the transgene. In transgenics that express low A-subunit levels (Lo-expressors), regulatory T cell (Treg) depletion using anti-CD25 before ...
Sandra M McLachlan +4 more
doaj +1 more source
ISOLATION OF A PROTEIN SUBUNIT FROM MICROTUBULES [PDF]
The Journal of Cell Biology, 1967 Sea-urchin sperm tails (Strongylocentrotus purpuratus) were obtained by amputation in synthetic sea water and were purified by differential centrifugation. Most of the arms of the outer nine doublets and soluble matrix proteins were removed by this treatment. The central pairs of microtubules were dissolved by dialysis against EDTA at pH 7.5.
M L, Shelanski, E W, Taylor
openaire +2 more sources
Scientific Reports, 2023 We developed a new method to analyze protein–protein interactions using a dual-inducible prokaryotic expression system. To evaluate protein–protein binding, a chimeric fusion toxin gene was constructed using a DNase-treated short DNA fragment (epitope ...
Agnieszka M. Murakami +5 more
doaj +1 more source
Analytical Insights into Protein–Alum Interactions and Their Impact on Conformational Epitope
PharmaceuticsSeveral alum-adjuvanted vaccines have been licensed in the past 40 years. Despite its extensive and continuous use, the immune mechanism of action of alum adjuvants is not yet completely understood. Many different variables during the formulation process
Alessio Corrado +7 more
doaj +1 more source
Viruses, 2018 An atypical porcine pestivirus (APPV) causing congenital tremor type A-II in piglets was identified in China in 2016. An increased number of cases of APPV have been reported in various countries all over the world since 2015.
Huawei Zhang +5 more
doaj +1 more source
Journal of Marine Science and Engineering, 2023 Tetrodotoxin (TTX)-binding proteins are present in toxic TTX-bearing animals, such as pufferfish and gastropods. These may prevent autotoxicity. However, TTX-binding proteins are also found in the nontoxic marine shore crab, Hemigrapsus sanguineus. Here,
Yuji Nagashima +5 more
doaj +1 more source
Protein Kinase A Regulatory Subunits in Colon Cancer
Neoplasia: An International Journal for Oncology Research, 1999 The protein kinase A (PKA) is classified as type I or II depending on the association of the catalytic subunit with either the RI or RII regulatory subunits.
Chris C. Carlson +4 more
doaj +1 more source