Results 71 to 80 of about 1,594,666 (333)

The carboxylate “gripper” of the substrate is critical for C‐4 stereo‐inversion by UDP‐glucuronic acid 4‐epimerase

FEBS Letters, EarlyView.
UDP‐glucuronic acid 4‐epimerase (UGAepi) catalyzes NAD+‐dependent interconversion of UDP‐glucuronic acid (UDP‐GlcA) and UDP‐galacturonic acid (UDP‐GalA) via C4‐oxidation, 4‐keto‐intermediate rotation, and C4‐reduction. Here, Borg et al. examined the role of the substrate's carboxylate group in the enzymic mechanism by analyzing NADH‐dependent reduction
Annika J. E. Borg, Laura De Cnop, Bernd Nidetzky   +2 more
wiley   +1 more source

Particle transport across a channel via an oscillating potential [PDF]

, 2017
Membrane protein transporters alternate their substrate-binding sites between the extracellular and cytosolic side of the membrane according to the alternating access mechanism.
Dagdug, Leonardo, Gladrow, Jannes, Keyser, Ulrich F., Pagliara, Stefano, Tan, Yizhou   +4 more
core   +2 more sources

Trafficking and localization of Golgi-resident N-glycan processing enzymes in plants

Frontiers in Plant Science
Asparagine (N)-linked glycosylation is a fundamental co- and post-translational modification of proteins, playing a crucial role in protein folding, stability and function, protein-protein interactions, biotic and abiotic stress response as well as ...
Kai Dünser, Jennifer Schoberer
doaj   +1 more source

In Vitro Reconstitution of Functional Type III Protein Export and Insights into Flagellar Assembly

mBio, 2018
The type III secretion system (T3SS) forms the functional core of injectisomes, protein transporters that allow bacteria to deliver virulence factors into their hosts for infection, and flagella, which are critical for many pathogens to reach the site of
Hiroyuki Terashima, Akihiro Kawamoto, Chinatsu Tatsumi, Keiichi Namba, Tohru Minamino, Katsumi Imada   +5 more
doaj   +1 more source

Homology Modeling of Transporter Proteins

, 2023
Membrane transporter proteins are divided into channels/pores and carriers and constitute protein families of physiological and pharmacological importance. Several presently used therapeutic compounds elucidate their effects by targeting membrane transporter proteins, including anti-arrhythmic, anesthetic, antidepressant, anxiolytic and diuretic drugs.
Sylte, Ingebrigt, Gabrielsen, Mari, Kristiansen, kurt   +2 more
openaire   +3 more sources

B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses

FEBS Letters, EarlyView.
B cells sense external mechanical forces and convert them into biochemical signals through mechanotransduction. Understanding how malignant B cells respond to physical stimuli represents a groundbreaking area of research. This review examines the key mechano‐related molecules and pathways in B lymphocytes, highlights the most relevant techniques to ...
Marta Sampietro, Marco Cellani, Cristina Scielzo   +2 more
wiley   +1 more source

Na+-dependent and Na+-independent betaine transport across the apical membrane of rat renal epithelium [PDF]

, 2015
The low renal excretion of betaine indicates that the kidney efficiently reabsorbs the betaine filtered by the glomeruli but the mechanisms involved in such a process have been scarcely investigated.
Calonge Castrillo, María Luisa, Cano Rodríguez, María Mercedes, Ilundáin Larrañeta, María Anunciación Ana   +2 more
core   +1 more source

CoCl2‐induced glioma hypoxia environment enhances CD47‐SIRPα to promote tumor immune evasion

MedComm – Oncology
Elevated levels of tumor‐associated macrophages and microglia in the immune microenvironment of malignant gliomas promote tumor growth and progression.
Xiangfei Ding, Jianbing Hou, Xiaosong Hu, Wen Peng, Yongsen Li, Gaichao Zhao, Hongjuan Cui, Zonghui Wu, Ping Liang   +8 more
doaj   +1 more source

In vivo evidence for glycyl radical insertion into a catalytically inactive variant of pyruvate formate‐lyase

FEBS Letters, EarlyView.
Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel, A. F. Volker Wagner, R. Gary Sawers   +2 more
wiley   +1 more source

Sac1p mediates the adenosine triphosphate transport into yeast endoplasmic reticulum that is required for protein translocation. [PDF]

, 1995
Protein translocation into the yeast endoplasmic reticulum requires the transport of ATP into the lumen of this organelle. Microsomal ATP transport activity was reconstituted into proteoliposomes to characterize and identify the transporter protein.
Bankaitis, VA, Mayinger, P, Meyer, DI
core