Multistep protein unfolding during nanopore translocation.
Nature Nanotechnology, 2013 David Rodriguez-Larrea, H. Bayley
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Protein unfolding and refolding as transitions through virtual states [PDF]
, 2014 Single-molecule atomic force spectroscopy probes elastic properties of titin, ubiquitin and other relevant proteins. We explain bioprotein folding dynamics under both length- and force-clamp by modeling polyprotein modules as particles in a bistable ...
Bonilla, L. L., Carpio, A., Prados, A.
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Structures of the ATP-fueled ClpXP proteolytic machine bound to protein substrate
eLife, 2020 ClpXP is an ATP-dependent protease in which the ClpX AAA+ motor binds, unfolds, and translocates specific protein substrates into the degradation chamber of ClpP. We present cryo-EM studies of the E.
Xue Fei +6 more
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Mechanotransduction in talin through the interaction of the R8 domain with DLC1.
PLoS Biology, 2018 The mechanical unfolding of proteins is a cellular mechanism for force transduction with potentially broad implications in cell fate. Despite this, the mechanism by which protein unfolding elicits differential downstream signalling pathways remains ...
Alexander William M Haining +7 more
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Engineering the kinetic stability of a β-trefoil protein by tuning its topological complexity
Frontiers in Molecular Biosciences, 2023 Kinetic stability, defined as the rate of protein unfolding, is central to determining the functional lifetime of proteins, both in nature and in wide-ranging medical and biotechnological applications.
Delaney M. Anderson +3 more
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Polar residues in the protein core of Escherichia coli thioredoxin are important for fold specificity [PDF]
, 2001 Most globular proteins contain a core of hydrophobic residues that are inaccessible to solvent in the folded state. In general, polar residues in the core are thermodynamically unfavorable except when they are able to form intramolecular hydrogen bonds ...
Bolon, Daniel N., Mayo, Stephen L.
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The Effect of Dimethyl Sulfoxide on the Lysozyme Unfolding Kinetics, Thermodynamics, and Mechanism
Biomolecules, 2019 The thermal stability of proteins in the presence of organic solvents and the search for ways to increase this stability are important topics in industrial biocatalysis and protein engineering.
Timur Magsumov +3 more
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Journal of the American Society for Mass Spectrometry, 2018 The initial stages of protein unfolding may reflect the stability of the entire fold and can also reveal which parts of a protein can be perturbed, without restructuring the rest. In this work, we couple UVPD with activated ion mobility mass spectrometry
A. Theisen +5 more
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Optimizing the calculation of energy landscape parameters from single-molecule protein unfolding experiments [PDF]
, 2015 Single-molecule force spectroscopy using an atomic force microscope (AFM) can be used to measure the average unfolding force of proteins in a constant velocity experiment.
David J. Brockwell +7 more
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Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response
Molecular Biology of the Cell, 2018 In eukaryotic cells, organelle-specific stress-response mechanisms are vital for maintaining cellular homeostasis. The Golgi apparatus, an essential organelle of the secretory system, is the major site of protein modification and sorting within a cell ...
Yevgeniy V. Serebrenik +5 more
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