Results 61 to 70 of about 394,740 (302)
Bio-ProtocolThe cellular secretome is a rich source of biomarkers and extracellular signaling molecules, but proteomic profiling remains challenging, especially when processing culture volumes greater than 5 mL.
Basil Baby Mattamana +4 more
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Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis
Nature Communications, 2018 Microtubule asters originate from centrosomes but their role during interphase remains largely unknown. Here, the authors find that microtubule asters anchored by previously-uncharacterized FSD1 play a role in ciliogenesis by maintaining the dynamic ...
Hai-Qing Tu +16 more
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Cardiovascular Diabetology, 2018 NG-Methylation of l-arginine (Arg) residues in certain proteins by protein arginine methyltransferases and subsequent proteolysis yields NG-monomethyl-l-arginine (MMA), NG,NG-dimethyl-l-arginine (asymmetric dimethylarginine, ADMA) and NG,N′G-dimethyl-l ...
Dimitrios Tsikas +3 more
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Neuropeptide reporter assay for serum, capillary blood and blood cards
MethodsX, 2020 In the associated main paper (JPBA 2019), substance P was shown to be a valuable neuropeptide reporter substance to monitor the protease activity of serum. The assay was developed based on the predecessor assay using bradykinin (JPBA 2017).
U. Schreiber +3 more
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A proteomic glimpse into human ureter proteome [PDF]
PROTEOMICS, 2015 Urine has evolved as one of the most important biofluids in clinical proteomics due to its noninvasive sampling and its stability. Yet, it is used in clinical diagnostics of several disorders by detecting changes in its components including urinary protein/polypeptide profile.
Magdeldin, Sameh +8 more
openaire +2 more sources
FEBS Letters, EarlyView.CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens +8 more
wiley +1 more source
Current challenges in software solutions for mass spectrometry-based quantitative proteomics [PDF]
, 2012 This work was in part supported by the PRIME-XS project, grant agreement number 262067, funded by the European Union seventh Framework Programme; The Netherlands Proteomics Centre, embedded in The Netherlands Genomics Initiative; The Netherlands ...
Heck, AJR +9 more
core +1 more source
An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes
FEBS Letters, EarlyView.GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg +14 more
wiley +1 more source
Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics
FEBS Letters, EarlyView.Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt +8 more
wiley +1 more source
Communications BiologyMouse is the mammalian model of choice to study human health and disease due to its size, ease of breeding and the natural occurrence of conditions mimicking human pathology. Here we design and validate multiple reaction monitoring mass spectrometry (MRM-
Sarah A. Michaud +16 more
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