Results 11 to 20 of about 7,610 (197)
Proton translocation driven by ATP hydrolysis in V‐ATPases
The vacuolar H+‐ATPases (or V‐ATPases) are a family of ATP‐dependent proton pumps responsible for acidification of intracellular compartments and, in certain cases, proton transport across the plasma membrane of eukaryotic cells. They are multisubunit complexes composed of a peripheral domain (V1) responsible for ATP hydrolysis and an integral domain ...
Kawasaki-Nishi, Shoko +2 more
openaire +4 more sources
Proton translocating ATPases: Issues in structure and function
Stone, Dennis K., Xie, Xiao-Song
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The role of the host—Neutrophil biology
Abstract Neutrophilic polymorphonuclear leukocytes (neutrophils) are myeloid cells packed with lysosomal granules (hence also called granulocytes) that contain a formidable antimicrobial arsenal. They are terminally differentiated cells that play a critical role in acute and chronic inflammation, as well as in the resolution of inflammation and wound ...
Iain L. C. Chapple +4 more
wiley +1 more source
Localization of a Proton-Translocating ATPase on Sucrose Gradients [PDF]
Ionophore-stimulated ATPase activity and ATP-dependent quinacrine quench were enriched in parallel when microsomal vesicles were prepared from corn (Crow Single Cross Hybrid WF9-Mo17) roots and collected on a cushion of 10% dextran. Activities were highest in the apical 1.5 centimeters of the roots.
F M, Dupont +2 more
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Estimating the Rotation Rate in the Vacuolar Proton-ATPase in Native Yeast Vacuolar Membranes [PDF]
The rate of rotation of the rotor of the yeast vacuolar proton-ATPase (V-ATPase), relative to the stator or the steady parts of enzyme, is estimated in native vacuolar membrane vesicles of Saccharomyces cerevisiae under standardised conditions.
Bóta, A. +9 more
core +1 more source
The microbiologist’s guide to membrane potential dynamics [PDF]
All cellular membranes have the functionality of generating and maintaining the gradients of electrical and electrochemical potentials. Such potentials were generally thought to be an essential but homeostatic contributor to complex bacterial behaviors ...
Asally, Munehiro, Benarroch, Jonatan M.
core +1 more source
Electrogenic Proton Translocation by the ATPase of Sugarcane Vacuoles [PDF]
Existence of a proton-translocating ATPase on the tonoplast of higher plants has been further confirmed by use of two experimental systems: (a) intact isolated vacuoles from sugarcane cells and (b) vesicles prepared from the same source. Addition of MgATP to vacuoles polarized the tonoplast by 40 millivolts to a value of +20 millivolts, but a large ...
M, Thom, E, Komor
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A cell-based assay for CD63-containing extracellular vesicles [PDF]
Extracellular vesicles (EVs) are thought to be important in cell-cell communication and have elicited extraordinary interest as potential biomarkers of disease. However, quantitative methods to enable elucidation of mechanisms underlying release are few.
Cashikar, Anil G, Hanson, Phyllis I
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Effect of antihypertensive agents - captopril and nifedipine - on the functional properties of rat heart mitochondria [PDF]
Objective(s): Investigation of acute effect on cellular bioenergetics provides the opportunity to characterize the possible adverse effects of drugs more comprehensively.
Ivana Kancirová +5 more
doaj
Proton translocation induced by ATPase activity in chloroplasts [PDF]
Proton uptake by chloroplasts was induced by light‐triggered ATPase activity. A quotient of two was obtained when the initial rate of proton uptake was divided by the rate of Pi released from ATP. Gramicidin accelerated the rate of ATPase activity and reduced the H+/Pi ratio to 1.4. The results were found to be consistent with the chemiosmotic theory.
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