Results 21 to 30 of about 5,860 (202)

Differential polypeptide synthesis of the proton-translocating ATPase of Escherichia coli [PDF]

Journal of Bacteriology, 1982
We investigated the regulation of the synthesis of the eight polypeptides of the Escherichia coli proton-translocating ATPase. A plasmid carrying the eight genes of the unc operon was used to direct in vivo and in vitro protein synthesis of the eight polypeptides.
W S, Brusilow, D J, Klionsky, R D, Simoni   +2 more
openaire   +2 more sources

Natural Products Inspired Scaffold Diversification Leads to Unnatural Molecular Warhead and Covalent Strategy to Modulating Protein Function through Electrophilic Bromine Transfer

Advanced Science, EarlyView.
We report a new thiolate‐reactive α,α‐gem‐dibromo lactam warhead that activates transcription factor Nrf2 and demonstrates anti‐inflammatory activities, which have implications in cancer, neurodegeneration, and cardiovascular diseases. RNA‐seq illuminated detailed transcriptional profiles, and chemical reactions with cysteine‐containing compounds ...
Beau R. Brummel, Sofia Kokkaliari, Qiwen Gao, Jinjing Chen, Larissa Costa de Almeida, Srinivasarao Tenneti, Derek A. Leas, Eli Levit, Lewis S. Alexander, Khalil A. Abboud, Pingrong Wei, Michael D. Cameron, James R. Rocca, Ranjala Ratnayake, Donna D. Zhang, Hendrik Luesch, Robert W. Huigens III   +16 more
wiley   +1 more source

Endogenous Engineering Reprograms Extracellular Vesicles for Enhanced Therapeutic Function

Advanced Science, EarlyView.
This review explains how Extracellular vesicles‐producing cells can be endogenously engineered to load therapeutic proteins and nucleic acids. We summarize physiological and genetic strategies that harness native sorting pathways for selective cargo loading.
Jinghui Wang, Wenxuan Zhao, Xiaoming Hu, Yishu Wang, Peidong Yang, Diwei Zheng, Yugang Wang, Guanghui Ma, Min Shi, Wei Wei, Shuang Wang   +10 more
wiley   +1 more source

An ancient pathway combining carbon dioxide fixation with the generation and utilization of a sodium ion gradient for ATP synthesis

, 2012
Synthesis of acetate from carbon dioxide and molecular hydrogen is considered to be the first carbon assimilation pathway on earth. It combines carbon dioxide fixation into acetyl-CoA with the production of ATP via an energized cell membrane.
Kaster, Anne-Kristin, Meike Goenrich, Gerhard Gottschalk, Schmidt, Silke, Volker Müller, Hecker, M., Schmidt, S., John Vollmers, Silke Schmidt (46446), Anja Poehlein, Andrea Thürmer, Gerhard Gottschalk (34564), Vollmers, John, Thauer, R., Thurmer, A., Goenrich, Meike, Schuchmann, Kai, Michael Hecker, Meike Goenrich (174838), Thauer, Rudolf K., Rudolf K. Thauer (174856), Volker Müller (49251), Gottschalk, G., Hecker, Michael, Müller, Volker, Gottschalk, Gerhard, Bertsch, J., Daniel, R., Muller, V., Silke Schmidt, Birgit Voigt (174853), Poehlein, Anja, Schuchmann, K., Goenrich, M., Rudolf K Thauer, Andrea Thürmer (174844), Johannes Bertsch, Anne-Kristin Kaster (174829), Rolf Daniel (113374), Bertsch, Johannes, Johannes Bertsch (174847), Vollmers, J., Birgit Voigt, Kai Schuchmann (174850), Thürmer, Andrea, John Vollmers (174842), Rolf Daniel, Daniel, Rolf, Kaster, A., Anne-Kristin Kaster, Voigt, Birgit, Kai Schuchmann, Anja Poehlein (113372), Michael Hecker (51971), Voigt, B., Poehlein, A.   +55 more
core   +1 more source

A Viscous DES‐AAV‐Foxo1 Delivery System With High Transfection Efficiency for the Treatment of Corneal Endothelial Dysfunction by Restoring Mitochondria‐ER Contacts

Advanced Science, EarlyView.
High glucose triggers corneal endothelial dysfunction by impairing FOXO1‐mediated ITPR1 transcription, leading to disrupted mitochondria‐associated membrane (MAM) integrity and defective ER‐to‐mitochondria Ca2+ transfer. This study develops an innovative viscous DES‐AAV‐Foxo1 delivery system with enhanced transfection efficiency. This non‐invasive gene
Hongran Zhao, Xiaoyu Li, Hongwei Wang, Zongyi Li, Qun Wang, Yangyang Zhang, Xu Jing, Xia Qi, Qingjun Zhou, Shengqian Dou, Lixin Xie   +10 more
wiley   +1 more source

Therapeutic Applications of Stimuli‐Based Release and Engineering of Extracellular Vesicles

Advanced NanoBiomed Research, EarlyView.
This review summarizes the effects of endogenous and exogenous stimuli, their effects on the natural release of extracellular vesicles, as well as their uptake and release. It also gives an overview of stimuli‐responsive EVs and their therapeutic applications. Extracellular vesicles (EVs), nano‐ to microsized lipid bilayer membrane‐bound particles, are
Gloria Kemunto, Kristen Dellinger
wiley   +1 more source

I hereby declare that the work presented in this document is based on research carried out by me, and that no part has previously been submitted for a degree in this or any other university [PDF]

, 1995
Inhibitors of known transport processes, in conjunction with ion substitution were used in biochemical, physiological and microelectrode studies to investigate the mechanisms underlying ion and fluid secretion across the Malpighian tubules of Locusta ...
Marshall, Sarah Louise
core  

The Lack of a COPII Cargo Receptor Erv14 Impacts Physiological Functions of the Vacuole in Saccharomyces cerevisiae. [PDF]

Traffic
Loss of the cargo receptor Erv14 in Saccharomyces cerevisiae results in sensitivity to calcium, zinc, neutral extracellular pH, and elevated temperature. Furthermore, cells deleted in the ERV14 exhibits vacuolar fragmentation, enlarged lipid droplets, and metabolic alterations. Ruiz Salas, J. L. (2026) https://BioRender.com/b65g523. ABSTRACT Erv14 is a
Rosas-Santiago P, Salas JLR, Cordoba E, Portillo FV, Salas-Navarrete PC, Cabrera JAP, Martínez A, Ramírez NIR, Hernández DC, Saralegui-Amaro A, Calahorra M, Sychrová H, Zimmermannová O.   +12 more
europepmc   +2 more sources

Conformation of a Peptide Encompassing the Proton Translocation Channel of Vacuolar H+-ATPase [PDF]

Biophysical Journal, 2007
The structural properties of a crucial transmembrane helix for proton translocation in vacuolar ATPase are studied using double site-directed spin-labeling combined with electron spin resonance (ESR) (or electron paramagnetic resonance) and circular dichroism spectroscopy in sodium dodecyl sulfate micelles.
Vos, W.L., Vermeer, L.S., Hemminga, M.A.
openaire   +3 more sources

Biomaterial design strategies for enhancing mitochondrial transplantation therapy

BMEMat, EarlyView.
Biomaterials to facilitate mitochondrial transplantation therapy: biomaterials as barriers to protect mitochondria from pathophysiological microenvironments, like osmotic stress caused by the excessive concentration of calcium ion, reactive oxygen species, and advanced glycation end products; biomaterials integrating with biochemical cues to improve ...
Shaoyang Kang, Yushan Zhang, Hang Li, Sirui Peng, Donghao Lyu, Chunxiao Zhou, Sheng Ding, Zujian Feng, Pingsheng Huang, Chuangnian Zhang, Hongjun Wang, Deling Kong, Weiwei Wang   +12 more
wiley   +1 more source