Results 301 to 310 of about 54,280 (357)
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Lactobacillus protoplast transformation
Plasmid, 1987A method for the transformation of Lactobacillus protoplasts by plasmid DNA is reported. The procedure involves polyethylene glycol treatment of protoplasts to induce DNA uptake. A transformation efficiency ranging from 5 to 1000 transformants per microgram of DNA is achieved; the efficiency of protoplast regeneration ranged from 10 to 20%.
L, Morelli +5 more
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Nature, 1974
RESEARCH on fusion of cells of higher eukaryotes is in an advanced stage and excellent reviews have been published concerning fusion of both animal cells1–4 and plant protoplasts5–7. On the other hand, no information is available regarding the results of fusion of fungal protoplasts, in spite of its possibilities for basic and applied research. Here we
L, Ferenczy, F, Kevei, J, Zsolt
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RESEARCH on fusion of cells of higher eukaryotes is in an advanced stage and excellent reviews have been published concerning fusion of both animal cells1–4 and plant protoplasts5–7. On the other hand, no information is available regarding the results of fusion of fungal protoplasts, in spite of its possibilities for basic and applied research. Here we
L, Ferenczy, F, Kevei, J, Zsolt
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VACUOLES OF FUNGAL PROTOPLASTS
Canadian Journal of Microbiology, 1967Details are given of the conditions which allow vacuolar structures of Fusarium culmorum to be released after osmotic bursting of mycelial protoplasts. Steps in the release of vacuoles are shown. The composition of the suspending medium affects the formation, size, and number of the vacuoles seen in the protoplasts.
I, Garcia-Acha +2 more
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1985
In its strictest sense a viable protoplast is one capable of regeneration into a plant, a capacity that has been demonstrated for few species and, with a few exceptions, for none in the conifers and monocotyledonous plants. In such cases where whole plant regeneration is potentially possible, there is no single parameter of viability, or collection of ...
Bornman, Chris H, Bornman, Janet
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In its strictest sense a viable protoplast is one capable of regeneration into a plant, a capacity that has been demonstrated for few species and, with a few exceptions, for none in the conifers and monocotyledonous plants. In such cases where whole plant regeneration is potentially possible, there is no single parameter of viability, or collection of ...
Bornman, Chris H, Bornman, Janet
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Protoplast regeneration and organogenesis from pea protoplasts
In Vitro Cellular & Developmental Biology, 1989Protoplasts of young Pisum Sativum L. seedlings from 7 different genotypes were isolated and regenerated to the callus stage. Germinating embryos were cultivated with cotyledons removed, thus avoiding intracellular starch accumulation in donor tissue.
Renate Lehminger-Mertens +1 more
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Protoplast Isolation and Staining
2015The successful isolation of mesophyll protoplasts from plant species has become a versatile tool for in vivo imaging of subcellular structures. Taking advantage of the various cytochemical probes available, the subcellular localization of specific organelles can be visualized in live protoplasts.
Liu, MQ +6 more
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Haploid protoplasts: pollen protoplasts
1996The experimental significance of haploid protoplasts requires no explanation, and their biological potential was well reviewed by Bajaj (1983) about 10 years ago. Since the totipotency of haploid cells of higher plants was first demonstrated by the production of haploids derived from pollen grains in anther culture (Guha and Maheshwari, 1964), many ...
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Wheat protoplast culture: embryogenic colony formation from protoplasts
Plant Cell Reports, 1988Wheat (Triticum aestivum L. cv Chinese Spring) protoplasts were isolated from immature embryos or embryogenic calli (3-4 weeks of culture on MS medium with 32 mg/1 dicamba) and cultured in R2 medium containing 2 mg/1 2,4-D by the nurse culture methods originally developed for rice protoplasts (Kyozuka et al. 1987). Protoplasts isolated from embryogenic
Y, Hayashi, K, Shimamoto
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Protoplast Fusion and Protoplast Transformation in Micromonospora Species
1983It is well known that Micromonospora species produce important antibiotics but very few genetic works have been done with them. The new genetic manipulations are more frequently applied in the recent years in the strain improvement programs of antibiotics producing microorganisms.
M. Szigetvári-Gabányi, M. Járai
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Bacterial protoplast fusion: Recombination in fused protoplasts of Streptomyces coelicolor
Molecular and General Genetics MGG, 1978Numerous recombinants arose when protoplasts of S. coelicolor were treated with polyethylene glycol and regenerated on non-selective solid medium. In six-factor crosses, recombination frequencies of more than 10% (up to 17%) were routinely observed. This recombination did not require either of the known sex factors, SCPI and SCP2.
D A, Hopwood, H M, Wright
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