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Transient Expression in Protoplasts
Cold Spring Harbor Protocols, 2007INTRODUCTIONThe study of transient gene expression provides a useful complement to the study of stably transformed plants. Transient assays offer a quick method of testing the effects of genes, using either phenotypic, molecular, or biochemical readouts.
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Totipotency of tomato protoplasts
Theoretical and Applied Genetics, 1985An efficient and reliable protocol for tomato protoplast isolation, culture, and plant regeneration has been developed. Fourteen diverse cultivars were tested. Fertile plants were regenerated from all 14 cultivars without any modification in the protocol. Plating efficiency (percentage of the protoplasts that formed minicalli) of up to 50% was achieved.
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2003
It is now possible to regenerate plants from protoplasts of a wide range of species. As a result, genetic manipulation by protoplast fusion in vitro is now a realistic proposition. This area is exciting because protoplasts from different origins can be fused together to form new genome combinations that cannot be obtained by conventional means.
N, Fish, K, Lindsey, M G, Jones
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It is now possible to regenerate plants from protoplasts of a wide range of species. As a result, genetic manipulation by protoplast fusion in vitro is now a realistic proposition. This area is exciting because protoplasts from different origins can be fused together to form new genome combinations that cannot be obtained by conventional means.
N, Fish, K, Lindsey, M G, Jones
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Protoplasts and Gravireactivity
1985The direction of growth of plant organs and the differentiation of higher plants depends to a large extent on gravity. When the various organs are turned away from their normal position with respect to gravity, they perform gravitropic (geotropic) movements which enable them to approach their normal orientation.
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Protoplast Isolation, Fusion, Culture and Transformation in the Woody Plant Jasminum spp.
Agriculture (Switzerland), 2021Mohamed A A Ahmed +2 more
exaly
Uptake of Fungal Protoplasts by Plant Protoplasts
1989P. T. Lynch, S. Isaac, H. A. Collin
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