Results 21 to 30 of about 18,625 (239)

Parallel analysis of transcription, integration, and sequence of single HIV-1 proviruses

Cell, 2022
Summary HIV-1-infected cells that persist despite antiretroviral therapy (ART) are frequently considered “transcriptionally silent,” but active viral gene expression may occur in some cells, challenging the concept of viral latency. Applying an assay for
Kevin B. Einkauf, Matt Osborn, Ce Gao, Weiwei Sun, Xiaoming Sun, Xiaodong Lian, Elizabeth M. Parsons, G. Gladkov, Kyra W. Seiger, Jane E. Blackmer, Chenyang Jiang, S. Yukl, E. Rosenberg, Xu G. Yu, M. Lichterfeld   +14 more
semanticscholar   +1 more source

Endogenous proviruses as “mementos”? [PDF]

Nature, 1997
In a recent News and Views1, J. P. Stoye discussed the potential health risks associated with the spread of endogenous proviruses from pigs and primates through the use of their organs for transplantation into humans. Although the risks to patients and the public from horizontal transmission may be as manageable as he represents, we would like to ...
Charles H. Langley, Brian Charlesworth
openaire   +3 more sources

Long-term persistence of transcriptionally active ‘defective’ HIV-1 proviruses: implications for persistent immune activation during antiretroviral therapy

AIDS (London), 2023
Objectives: People with HIV-1 (PWH) on effective antiretroviral therapy (ART) continue to exhibit chronic systemic inflammation, immune activation, and persistent elevations in markers of HIV-1 infection [including HIV-DNA, cell-associated HIV-RNA (CA ...
Kanal Singh, V. Natarajan, R. Dewar, Adam W. Rupert, Yuden Badralmaa, Tracey Zhai, Nicole E Winchester, F. Scrimieri, Mindy Smith, I. Davis, P. Lallemand, Aude Giglietti, Jack M. Hensien, T. Buerkert, Bruktawit A. Goshu, C. Rehm, Zonghui Hu, H. Lane, H. Imamichi   +18 more
semanticscholar   +1 more source

Persistence of intact HIV-1 proviruses in the brain during antiretroviral therapy

bioRxiv, 2023
HIV-1 reservoir cells that circulate in peripheral blood during suppressive antiretroviral therapy (ART) have been well characterized, but little is known about the dissemination of HIV-1-infected cells across multiple anatomical tissues, especially the ...
Weiwei Sun, Yelizaveta Rassadkina, Ce Gao, Sarah I. Collens, Xiaodong Lian, I. Solomon, S. Mukerji, Xu G. Yu, M. Lichterfeld   +8 more
semanticscholar   +1 more source

The replication-competent HIV reservoir is a genetically restricted, younger subset of the overall pool of HIV proviruses persisting during therapy, which is highly genetically stable over time

Research Square, 2023
Within-host HIV populations continually diversify during untreated infection, and this diversity persists within infected cell reservoirs during antiretroviral therapy (ART).
A. Shahid, Signe MacLennan, B. Jones, H. Sudderuddin, Zhong Dang, K. Cobarrubias, M. Duncan, N. Kinloch, M. J. Dapp, N. Archin, M. Fischl, I. Ofotokun, A. Adimora, S. Gange, B. Aouizerat, M. Kuniholm, Seble G. Kassaye, J. Mullins, H. Goldstein, J. Joy, K. Anastos, Z. Brumme   +21 more
semanticscholar   +1 more source

Complex decay dynamics of HIV virions, intact and defective proviruses, and 2LTR circles following initiation of antiretroviral therapy

Proceedings of the National Academy of Sciences of the United States of America, 2022
Significance In persons living with HIV-1 who start antiretroviral therapy, virus in the blood decreases rapidly to below the detection limit. The decrease occurs in two phases: a rapid initial decrease in the first weeks, followed by a second, slower ...
J. White, F. Simonetti, Subul A. Beg, Natalie F. McMyn, W. Dai, N. Bachmann, J. Lai, William C. Ford, Chris Bunch, Joyce L. Jones, R. Ribeiro, A. Perelson, J. Siliciano, R. Siliciano   +13 more
semanticscholar   +1 more source

Intact HIV Proviruses Persist in the Brain Despite Viral Suppression with ART

Annals of Neurology, 2022
Human immunodeficiency virus (HIV) persistence in blood and tissue reservoirs, including the brain, is a major barrier to HIV cure and possible cause of comorbid disease.
Catherine R. Cochrane, T. Angelovich, Sarah J. Byrnes, Emily Waring, Aleks C Guanizo, Gemma S Trollope, Jingling Zhou, Judith Vue, Lachlan Senior, Emma Wanicek, Janna Jamal Eddine, Matthew J Gartner, T. Jenkins, P. Gorry, B. Brew, S. Lewin, J. Estes, Michael Roche, M. Churchill   +18 more
semanticscholar   +1 more source

Varied Interactions Between Proviruses and Adjacent Host Chromatin [PDF]

Molecular and Cellular Biology, 1986
Retroviruses integrated at unique locations in the host genome can be expressed at different levels. We have analyzed the preintegration sites of three transcriptionally competent avian endogenous proviruses (evs) to determine whether the various levels of provirus expression correlate with their location in active or inactive regions of chromatin. Our
K F, Conklin, M, Groudine
openaire   +4 more sources

Adaptation of the intact proviral DNA assay to a nanowell-based digital PCR platform

Journal of Virus Eradication, 2023
Quantification of intact proviruses is a critical measurement in HIV cure studies both in vitro and in vivo. The widely adopted ‘intact proviral DNA assay’ (IPDA), designed to discriminate and quantify genetically intact HIV proviruses based on detection
Carolin Tumpach, Catherine R. Cochrane, Youry Kim, Jesslyn Ong, Ajantha Rhodes, Thomas A. Angelovich, Melissa J. Churchill, Sharon R. Lewin, Sushama Telwatte, Michael Roche   +9 more
doaj   +1 more source

Sensitive detection systems for infectious agents in xenotransplantation*

Xenotransplantation, EarlyView., 2020
Abstract Xenotransplantation of pig cells, tissues, or organs may be associated with transmission of porcine microorganisms, first of all of viruses, to the transplant recipient, potentially inducing a disease (zoonosis). I would like to define detection systems as the complex of sample generation, sample preparation, sample origin, time of sampling ...
Joachim Denner
wiley   +1 more source