Results 41 to 50 of about 13,226 (233)

Adaptation of the intact proviral DNA assay to a nanowell-based digital PCR platform

Journal of Virus Eradication, 2023
Quantification of intact proviruses is a critical measurement in HIV cure studies both in vitro and in vivo. The widely adopted ‘intact proviral DNA assay’ (IPDA), designed to discriminate and quantify genetically intact HIV proviruses based on detection
Carolin Tumpach, Catherine R. Cochrane, Youry Kim, Jesslyn Ong, Ajantha Rhodes, Thomas A. Angelovich, Melissa J. Churchill, Sharon R. Lewin, Sushama Telwatte, Michael Roche   +9 more
doaj   +1 more source

Sensitive detection systems for infectious agents in xenotransplantation*

Xenotransplantation, EarlyView., 2020
Abstract Xenotransplantation of pig cells, tissues, or organs may be associated with transmission of porcine microorganisms, first of all of viruses, to the transplant recipient, potentially inducing a disease (zoonosis). I would like to define detection systems as the complex of sample generation, sample preparation, sample origin, time of sampling ...
Joachim Denner
wiley   +1 more source

Droplet microfluidic sequencing of HIV proviruses with their integration sites in patients receiving antiretroviral therapy

Nature Biomedical Engineering, 2022
The human immunodeficiency virus (HIV) integrates its genome into that of infected cells and may enter an inactive state of reversible latency that cannot be targeted using antiretroviral therapy.
Chen Sun, Leqian Liu, L. Pérez, Xiangpeng Li, Yifan Liu, Peng Xu, E. Boritz, J. Mullins, A. Abate   +8 more
semanticscholar   +1 more source

Deep Sequencing Analysis of Individual HIV-1 Proviruses Reveals Frequent Asymmetric Long Terminal Repeats

Journal of Virology, 2022
The integration of human immunodeficiency virus type 1 (HIV-1) into chromosomal DNA establishes the long-term persistence of HIV-1 as proviruses despite effective antiretroviral therapy (ART).
K. Joseph, E. Halvas, L. Brandt, S. Patro, J. Rausch, A. Chopra, S. Mallal, Mary F. Kearney, J. Coffin, J. Mellors   +9 more
semanticscholar   +1 more source

HIV-1 reservoir evolution in infants infected with clade C from Mozambique

International Journal of Infectious Diseases, 2023
Background: The persistence of HIV-1-infected cells during antiretroviral therapy is well documented but may be modulated by early initiation of antiretroviral therapy in infants.
Catherine K. Koofhethile, Stefano Rinaldi, Yelizaveta Rassadkina, Vinh B. Dinh, Ce Gao, Suresh Pallikkuth, Pilar Garcia-Broncano, Lesley R. de Armas, Rajendra Pahwa, Nicola Cotugno, Paula Vaz, Maria Grazia Lain, Paolo Palma, Xu G. Yu, Roger Shapiro, Savita Pahwa, Mathias Lichterfeld   +16 more
doaj   +1 more source

Signatures of immune selection in intact and defective proviruses distinguish HIV-1 elite controllers

Science Translational Medicine, 2021
Description Immune selection footprints in proviral sequences distinguish individuals with natural, drug-free control of HIV-1. Understanding elite controllers Some individuals living with HIV-1 are able to control viral loads without the need for ...
Xiao-lan Lian, Ce Gao, Xiaoming Sun, Chenyan Jiang, Kevin B. Einkauf, Kyra W. Seiger, Joshua M. Chevalier, Y. Yuki, Maureen P. Martin, R. Hoh, M. Peluso, Mary Carrington, E. Ruiz-Mateos, S. Deeks, E. Rosenberg, B. Walker, M. Lichterfeld, Xu G. Yu   +17 more
semanticscholar   +1 more source

Low Inducibility of Latent Human Immunodeficiency Virus Type 1 Proviruses as a Major Barrier to Cure.

Journal of Infectious Diseases, 2021
The latent reservoir for human immunodeficiency virus type 1 (HIV-1) in resting CD4+ T cells is a major barrier to cure. The dimensions of the reservoir problem can be defined with 2 assays.
J. Siliciano, R. Siliciano
semanticscholar   +1 more source

Tracking HIV-1-Infected Cell Clones Using Integration Site-Specific qPCR

Viruses, 2021
Efforts to cure HIV-1 infection require better quantification of the HIV-1 reservoir, particularly the clones of cells harboring replication-competent (intact) proviruses, termed repliclones.
Leah D. Brandt, Shuang Guo, Kevin W. Joseph, Jana L. Jacobs, Asma Naqvi, John M. Coffin, Mary F. Kearney, Elias K. Halvas, Xiaolin Wu, Stephen H. Hughes, John W. Mellors   +10 more
doaj   +1 more source

A linkage map of endogenous murine leukemia proviruses. [PDF]

Genetics, 1990
Abstract Thirty endogenous proviruses belonging to the modified polytropic (Mpmv) class of murine leukemia virus (MLV) were identified by proviral-cellular DNA junction fragment segregation in several sets of recombinant inbred mice. Twenty-six Mpmv loci were mapped to chromosomal regions by matching proviral strain distribution patterns
Frankel, W N, Stoye, J P, Taylor, B A, Coffin, J M   +3 more
openaire   +2 more sources

HIVIntact: a python-based tool for HIV-1 genome intactness inference

Retrovirology, 2021
The characterisation of the HIV-1 reservoir, which consists of replication-competent integrated proviruses that persist on antiretroviral therapy (ART), is made difficult by the rarity of intact proviruses relative to those that are defective.
Imogen A. Wright, Michael J. Bale, Wei Shao, Wei-Shau Hu, John M. Coffin, Gert U. Van Zyl, Mary F. Kearney   +6 more
doaj   +1 more source