Results 51 to 60 of about 13,226 (233)

A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoir

Cell Reports Medicine, 2021
Summary: Quantifying the replication-competent HIV reservoir is essential for evaluating curative strategies. Viral outgrowth assays (VOAs) underestimate the reservoir because they fail to induce all replication-competent proviruses.
Claire N. Levy, Sean M. Hughes, Pavitra Roychoudhury, Daniel B. Reeves, Chelsea Amstuz, Haiying Zhu, Meei-Li Huang, Yulun Wei, Marta E. Bull, Noah A.J. Cassidy, Jan McClure, Lisa M. Frenkel, Mars Stone, Sonia Bakkour, Elizabeth R. Wonderlich, Michael P. Busch, Steven G. Deeks, Joshua T. Schiffer, Robert W. Coombs, Dara A. Lehman, Keith R. Jerome, Florian Hladik   +21 more
doaj   +1 more source

Different human resting memory CD4+ T cell subsets show similar low inducibility of latent HIV-1 proviruses

Science Translational Medicine, 2020
Latent replication-competent HIV-1 proviruses in different memory T cell subsets show no differences in viral gene transcription after T cell activation.
K. J. Kwon, Andrew E Timmons, Srona Sengupta, F. Simonetti, Hao Zhang, R. Hoh, S. Deeks, J. Siliciano, R. Siliciano   +8 more
semanticscholar   +1 more source

Defective HIV-1 genomes and their potential impact on HIV pathogenesis

Retrovirology, 2022
Defective HIV-1 proviruses represent a population of viral genomes that are selected for by immune pressures, and clonally expanded to dominate the persistent HIV-1 proviral genome landscape.
Jeffrey Kuniholm, Carolyn Coote, Andrew J. Henderson   +2 more
doaj   +1 more source

Insights from Clonal Expansion and HIV Persistence in Perinatal Infections

mBio, 2021
The latent HIV reservoir forms early in the course of infection and is maintained for life despite effective antiretroviral treatment (ART), including early treatment.
Adit Dhummakupt, Deborah Persaud
doaj   +1 more source

Activation of HIV-1 proviruses increases downstream chromatin accessibility

iScience, 2022
It is unclear how the activation of HIV-1 transcription affects chromatin structure. We interrogated chromatin organization both genome-wide and nearby HIV-1 integration sites using Hi-C and ATAC-seq. In conjunction, we analyzed the transcription of the HIV-1 genome and neighboring genes.
Raven Shah, Christian M. Gallardo, Yoonhee H. Jung, Ben Clock, Jesse R. Dixon, William M. McFadden, Kinjal Majumder, David J. Pintel, Victor G. Corces, Bruce E. Torbett, Philip R. Tedbury, Stefan G. Sarafianos   +11 more
openaire   +2 more sources

Hide and seek: for HIV-infected CD4+ T cells, playing well comes with maturity

The Journal of Clinical Investigation, 2022
Antiretroviral therapy suppresses HIV replication but leaves a population of infected CD4+ T cells with integrated proviruses. While most of these proviruses contain defects, such as deletions, some intact proviruses persist and can reinitiate viral ...
Louise Leyre, R. Brad Jones
doaj   +1 more source

Insertional activation of STAT3 and LCK by HIV-1 proviruses in T cell lymphomas

Science Advances, 2021
HIV-1 proviruses in the first introns of STAT3 and LCK can contribute to the development of T cell lymphomas.
J. Mellors, Shuang Guo, Asma Naqvi, L. Brandt, Ling Su, Zhonghe Sun, K. Joseph, D. Demirov, E. Halvas, D. Butcher, Beth A. Scott, A. Hamilton, Marintha Heil, Baktiar O Karim, Xiaolin Wu, S. Hughes   +15 more
semanticscholar   +1 more source

The Nature of the HTLV-1 Provirus in Naturally Infected Individuals Analyzed by the Viral DNA-Capture-Seq Approach

Cell Reports, 2019
Summary: The retrovirus human T-cell leukemia virus type 1 (HTLV-1) integrates into the host DNA, achieves persistent infection, and induces human diseases.
Hiroo Katsuya, Saiful Islam, Benjy Jek Yang Tan, Jumpei Ito, Paola Miyazato, Misaki Matsuo, Yuki Inada, Saori C. Iwase, Yoshikazu Uchiyama, Hiroyuki Hata, Tomoo Sato, Naoko Yagishita, Natsumi Araya, Takaharu Ueno, Kisato Nosaka, Masahito Tokunaga, Makoto Yamagishi, Toshiki Watanabe, Kaoru Uchimaru, Jun-ichi Fujisawa, Atae Utsunomiya, Yoshihisa Yamano, Yorifumi Satou   +22 more
doaj   +1 more source

New Approaches to Multi-Parametric HIV-1 Genetics Using Multiple Displacement Amplification: Determining the What, How, and Where of the HIV-1 Reservoir

Viruses, 2021
Development of potential HIV-1 curative interventions requires accurate characterization of the proviral reservoir, defined as host-integrated viral DNA genomes that drive rebound of viremia upon halting ART (antiretroviral therapy).
Sean C. Patro, Aurelie Niyongabo, Frank Maldarelli, Mary F. Kearney   +3 more
doaj   +1 more source

Intact HIV-1 proviruses accumulate at distinct chromosomal positions during prolonged antiretroviral therapy

Journal of Clinical Investigation, 2019
Chromosomal integration of genome-intact HIV-1 sequences into the host genome creates a reservoir of virally infected cells that persists throughout life, necessitating indefinite antiretroviral suppression therapy.
Kevin B. Einkauf, Guinevere Q. Lee, Ce Gao, Radwa Sharaf, Xiaoming Sun, S. Hua, Samantha M. Y. Chen, Chenyan Jiang, Xiaodong Lian, Fatema Z. Chowdhury, E. Rosenberg, T. Chun, Jonathan Z. Li, Xu G. Yu, M. Lichterfeld   +14 more
semanticscholar   +1 more source