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Differentiation among isolates of prunus necrotic ringspot virus by transcript conformation polymorphism

Journal of Virological Methods, 1998
A method based on differences in electrophoretic mobility of RNA transcripts made from polymerase chain reaction (PCR) products was used for differentiation among virus isolates. A T7 RNA polymerase promoter was attached to amplified prunus necrotic ringspot virus (PNRSV) sequences by PCR.
S Spiegel, A Rösner
exaly   +3 more sources

Prunus necrotic ringspot virus as a multicomponent system

Virology, 1975
Abstract When centrifuged in sucrose density gradients, Prunus necrotic ringspot virus separated into three zones of nucleoprotein particles with sedimentation rates in 0.03 M Tris, pH 7.5, of 72, 90, and 95 S. Bottom particles were slightly infectious; middle and top particles were noninfectious.
L S, Loesch, R W, Fulton
openaire   +2 more sources

Studies on Prunus Necrotic Ringspot Virus (PNRSV) Occurring on Lily

Agricultural Sciences in China, 2007
This study was to molecular identify Prunus necrotic ringspot virus (PNRSV) occurring on lily. Lily corms were randomly selected and grown in the greenhouse. The total RNAs were extracted from younger leaves, and partial amplification of the CP gene was performed by RT-PCR with primer pairs specific for PNRSV.
Li-juan HAN, Wen-hong LIU
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VERTICAL TRANSMISSION OF PRUNUS NECROTIC RINGSPOT VIRUS BY GAMETES IN APRICOT

Acta Horticulturae, 2004
Prunus necrotic ring spot virus (PNRSV) transmission takes place in nature during flowering and pollination. It is thought to be transmitted through pollen and seeds although the exact mechanism is unknown. In a previous work, we demonstrated that in the case of nectarine, PNRSV is located inside the pollen grains, which would help to explain its ...
K. Amari, M.A. Sánchez-Pina, V. Pallás
openaire   +1 more source

Molecular Evidence for Recombination in Prunus Necrotic Ringspot Virus

Plant Molecular Biology Reporter, 2008
Genetic RNA recombination plays an important role in viral evolution. The evolutionary history of Prunus necrotic ringspot virus (PNRSV) has been extensively studied, but knowledge of recombination in its genome is still lacking. To investigate the recombination events in this virus, 67 accessions composed by 62 isolates retrieved from the databanks ...
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Prunus necrotic ringspot virus . [Distribution map].

Distribution Maps of Plant Diseases, 2006
Abstract A new distribution map is provided for Prunus necrotic ringspot virus Viruses: Bromoviridae: llavirus Hosts: Mainly Prunus spp., Rosa spp. and Rubus spp. Information is given on the geographical distribution in EUROPE, Albania, Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark ...
null CABI, null EPPO
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Survey of Prunus necrotic ringspot virus in Rose and Its Variability in Rose and Prunus spp.

Phytopathology®, 2001
A survey for viruses in rose propagated in Europe resulted in detection of only Prunus necrotic ringspot virus (PNRSV) among seven viruses screened. Four percent of cut-flower roses from different sources were infected with PNRSV. Progression of the disease under greenhouse conditions was very slow, which should make this virus easy to eradicate ...
Moury, Benoît   +4 more
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Characteristics of antigens and antibodies associated with the prunus necrotic ringspot virus immune system

Virology, 1965
Abstract Two antigens, V and Q, are associated with Prunus necrotic ringspot infections of woody and herbaceous hosts. Only V (virus) is considered to be infectious, and the antigens are closely related as shown by agar immunodiffusion and absorption tests. Rabbits immunized with V produce two antibody types. One type reacts with both V and Q and the
W R, ALLEN, J G, TREMAINE
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