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Studies on Prunus Necrotic Ringspot Virus (PNRSV) Occurring on Lily
Agricultural Sciences in China, 2007This study was to molecular identify Prunus necrotic ringspot virus (PNRSV) occurring on lily. Lily corms were randomly selected and grown in the greenhouse. The total RNAs were extracted from younger leaves, and partial amplification of the CP gene was performed by RT-PCR with primer pairs specific for PNRSV.
Li-juan HAN, Wen-hong LIU
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VERTICAL TRANSMISSION OF PRUNUS NECROTIC RINGSPOT VIRUS BY GAMETES IN APRICOT
Acta Horticulturae, 2004Prunus necrotic ring spot virus (PNRSV) transmission takes place in nature during flowering and pollination. It is thought to be transmitted through pollen and seeds although the exact mechanism is unknown. In a previous work, we demonstrated that in the case of nectarine, PNRSV is located inside the pollen grains, which would help to explain its ...
K. Amari, M.A. Sánchez-Pina, V. Pallás
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Prunus necrotic ringspot virus . [Distribution map].
Distribution Maps of Plant Diseases, 2006Abstract A new distribution map is provided for Prunus necrotic ringspot virus Viruses: Bromoviridae: llavirus Hosts: Mainly Prunus spp., Rosa spp. and Rubus spp. Information is given on the geographical distribution in EUROPE, Albania, Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark ...
null CABI, null EPPO
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Molecular Evidence for Recombination in Prunus Necrotic Ringspot Virus
Plant Molecular Biology Reporter, 2008Genetic RNA recombination plays an important role in viral evolution. The evolutionary history of Prunus necrotic ringspot virus (PNRSV) has been extensively studied, but knowledge of recombination in its genome is still lacking. To investigate the recombination events in this virus, 67 accessions composed by 62 isolates retrieved from the databanks ...
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Virology, 1965
Abstract Two antigens, V and Q, are associated with Prunus necrotic ringspot infections of woody and herbaceous hosts. Only V (virus) is considered to be infectious, and the antigens are closely related as shown by agar immunodiffusion and absorption tests. Rabbits immunized with V produce two antibody types. One type reacts with both V and Q and the
W R, ALLEN, J G, TREMAINE
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Abstract Two antigens, V and Q, are associated with Prunus necrotic ringspot infections of woody and herbaceous hosts. Only V (virus) is considered to be infectious, and the antigens are closely related as shown by agar immunodiffusion and absorption tests. Rabbits immunized with V produce two antibody types. One type reacts with both V and Q and the
W R, ALLEN, J G, TREMAINE
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The effect of prunus necrotic ringspot virus on field‐grown roses
Annals of Applied Biology, 1982SUMMARYThe effect of prunus necrotic ringspot virus (PNRSV; cherry serotype) on field‐grown maiden rose bushes cv. Fragrant Cloud was assessed in two trials during 1979 and 1980. PNRSV delayed the onset of flowering, reduced the size and number of blooms and increased the proportion of deformed flowers but had no effect on flower pigmentation or scent.
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Molecular characterization of two prunus necrotic ringspot virus isolates from Canada
Archives of Virology, 2012We determined the entire RNA1, 2 and 3 sequences of two prunus necrotic ringspot virus (PNRSV) isolates, Chr3 from cherry and Pch12 from peach, obtained from an orchard in the Niagara Fruit Belt, Canada. The RNA1, 2 and 3 of the two isolates share nucleotide sequence identities of 98.6%, 98.4% and 94.5%, respectively.
Hongguang, Cui +3 more
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In Vitro Elimination of Prunus Necrotic Ringspot Virus in a Plum Cultivar
1999Production of vegetatively propagated plant material is based on building up stocks from source plants. Viruses entering a propagation system at the breeding or germ plasm level are transmitted vertically down the propagation chain. The ultimate outcome of this process is virus-infected planting material.
S. Spiegel +7 more
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Improved detection of prunus necrotic ringspot virus by the polymerase chain reaction
European Journal of Plant Pathology, 1996The reverse transcription — polymerase chain reaction (RT-PCR) technique was used for detection of prunus necrotic ringspot virus in dormant peach trees which tested negative by ELISA. Total RNA extracted from bark tissue, using a lithium chloride based method, were used for reverse transcription and subsequent amplification of viral sequences. The PCR
S. Spiegel +5 more
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Journal of Plant Pathology, 2015
Prunus necrotic ringspot virus (PNRSV) was detected by PCR in Tunisian stone fruit germplasm collections. Peach had the highest infection rate reaching 50%, followed by apricot (46.8%), almond (35.5%) and plum (9.5%). The genetic variability of the CP gene of 10 PNRSV isolates was evaluated by SSCP analysis of PCR products and analysis of their ...
Mahfoudhi, N. +5 more
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Prunus necrotic ringspot virus (PNRSV) was detected by PCR in Tunisian stone fruit germplasm collections. Peach had the highest infection rate reaching 50%, followed by apricot (46.8%), almond (35.5%) and plum (9.5%). The genetic variability of the CP gene of 10 PNRSV isolates was evaluated by SSCP analysis of PCR products and analysis of their ...
Mahfoudhi, N. +5 more
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