The applicability of single and double-stranded transcript conformation polymorphism (TCP) for differentiation among Prunus necrotic ringspot virus (PNRSV) isolates was evaluated and compared with other molecular differentiation procedures.
Arie Rosner +4 more
doaj +3 more sources
Prunus necrotic ringspot virus (PNRSV) and Prune dwarf virus (PDV) are the most common pathogens in stone crop orchards. These diseases are easily transmitted with pollen and hence rapidly spread in orchards leading to stunting of trees, their increased ...
Liliia PAVLIUK +3 more
doaj +3 more sources
Transmission of prunus necrotic ringspot virus using plum pollen and thrips
SUMMARYPrunus necrotic ringspot virus (PNRSV) was transmitted to cucumber but not to peach seedlings after they were dusted with infective plum pollen and caged with 8–10 thrips per seedling for 24 h. When the pollen was taken from three plum trees shown by mechanical inoculation tests to have highly infective flower buds and pollen, the transmission ...
GREBER, R. S. +3 more
openaire +4 more sources
Pollen health is a key determinant of successful plant reproduction and crop yield. Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) are pollen-borne viruses of stone fruits, including peaches, primarily transmitted through vegetative ...
Mandeep Tayal +6 more
doaj +2 more sources
The Complete Nucleotide Sequence of RNA 3 of a Peach Isolate of Prunus Necrotic Ringspot Virus
The complete nucleotide sequence of RNA 3 of the PE-5 peach isolate of Prunus necrotic ringspot ilarvirus (PNRSV) was obtained from cloned cDNA. The RNA sequence is 1941 nucleotides and contains two open reading frames (ORFs). ORF 1 consisted of 284 amino acids with a calculated molecular weight of 31,729 Da and ORF 2 contained 224 amino acids with a ...
Hammond, Rosemarie W. +1 more
openaire +3 more sources
Rapid Detection of Prunus Necrotic Ringspot Virus by Reverse Transcription-cross-priming Amplification Coupled with Nucleic Acid Test Strip Cassette. [PDF]
Prunus necrotic ringspot virus (PNRSV) is one of the most devastating viruses to Prunus spp. In this study, we developed a diagnostic system RT-CPA-NATSC, wherein reverse transcription-cross-priming amplification (RT-CPA) is coupled with nucleic acid ...
Huo YY, Li GF, Qiu YH, Li WM, Zhang YJ.
europepmc +2 more sources
Analysis of intra-host genetic diversity of Prunus necrotic ringspot virus (PNRSV) using amplicon next generation sequencing. [PDF]
PCR amplicon next generation sequencing (NGS) analysis offers a broadly applicable and targeted approach to detect populations of both high- or low-frequency virus variants in one or more plant samples. In this study, amplicon NGS was used to explore the
Kinoti WM +4 more
europepmc +3 more sources
Evaluating the Negative-Strand Coding Potential in Plum Pox Virus. [PDF]
Plum pox virus produces two polyproteins from ORFs in the positive strand. This study explores the possible expression of peptides from negative‐strand ORFs (rORFs). ABSTRACT Recent studies proposed that the negative strands of some single‐stranded, positive‐sense RNA viruses contain reverse open reading frames (rORFs) that encode functional peptides ...
García B +8 more
europepmc +2 more sources
Adaptive covariation between the coat and movement proteins of prunus necrotic ringspot virus. [PDF]
ABSTRACT The relative functional and/or structural importance of different amino acid sites in a protein can be assessed by evaluating the selective constraints to which they have been subjected during the course of evolution. Here we explore such constraints at the linear and three-dimensional levels for the movement protein (MP) and coat ...
Codoñer FM, Fares MA, Elena SF.
europepmc +5 more sources
Molecular characterization and intermolecular interaction of coat protein of Prunus necrotic ringspot virus: implications for virus assembly [PDF]
Saurabh Kulshrestha +2 more
exaly +2 more sources

