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Comparative void-volume analysis of psychrophilic and mesophilic enzymes: Structural bioinformatics of psychrophilic enzymes reveals sources of core flexibility [PDF]

open access: yesBMC Structural Biology, 2011
Background Psychrophiles, cold-adapted organisms, have adapted to live at low temperatures by using a variety of mechanisms. Their enzymes are active at cold temperatures by being structurally more flexible than mesophilic enzymes.
Kyle E Watters   +2 more
exaly   +5 more sources

Psychrophilic enzymes: a thermodynamic challenge

open access: yesBBA - Proteins and Proteomics, 1997
peer reviewedPsychrophilic microorganisms, hosts of permanently cold habitats, produce enzymes which are adapted to work at low temperatures. When compared to their mesophilic counterparts, these enzymes display a higher catalytic efficiency over a ...
Georges Feller, Etienne Baise, C Gerday
exaly   +4 more sources

Machine learning techniques for differentiating psychrophilic and non-psychrophilic bacterial α/β hydrolase enzymes

open access: yesBiologia (Poland)
Psychrophilic enzymes represent a category of macromolecules that have acquired specific properties that enable these enzymes to perform their catalytic activity at low temperatures with high efficiency.
Nurcan Vardar Yel
exaly   +4 more sources

Psychrophilic enzymes: revisiting the thermodynamic parameters of activation may explain local flexibility

open access: yesBBA - Proteins and Proteomics, 2000
peer reviewedBasic theoretical and practical aspects of activation parameters are briefly reviewed in the context of cold-adaptation. In order to reduce the error impact inherent to the transition state theory on the absolute values of the free energy ...
Georges Feller   +2 more
exaly   +4 more sources
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Psychrophilic enzymes: hot topics in cold adaptation

Nature Reviews Microbiology, 2003
More than three-quarters of the Earth's surface is occupied by cold ecosystems, including the ocean depths, and polar and alpine regions. These permanently cold environments have been successfully colonized by a class of extremophilic microorganisms that are known as psychrophiles (which literally means cold-loving).
Georges Feller, Charles Gerday
exaly   +4 more sources

Heat-labile Enzymes in a Psychrophilic Bacterium

Journal of Bacteriology, 1967
The oxidative and fermentative activities of a psychrophilic bacterium (strain 82), whose maximal growth temperature is 35 C, were completely destroyed by exposure of the cells to 46 C for about 1 hr, whereas those of mesophilicEscherichia coliwere unaffected. Similar results were obtained with cell-free extracts.
K, Purohit, J L, Stokes
openaire   +2 more sources

Enzyme Catalysis in Psychrophiles

2017
Cold-active enzymes are produced by organisms, known as psychrophiles, adapted to permanently cold habitats. Low temperatures have an exponential deleterious effct on reaction rates, and thus psychrophilic enzymes have to be adapted to secure appropriate reaction rates in their environment.
Collins, Tony, Gerday, Charles
openaire   +2 more sources

Did psychrophilic enzymes really win the challenge?

Extremophiles, 2001
Organisms living in permanently cold environments, which actually represent the greatest proportion of our planet, display at low temperatures metabolic fluxes comparable to those exhibited by mesophilic organisms at moderate temperatures. They produce cold-evolved enzymes partially able to cope with the reduction in chemical reaction rates and the ...
Zecchinon, Laurent   +11 more
openaire   +3 more sources

Comparative structural analysis of psychrophilic and meso‐ and thermophilic enzymes

Proteins: Structure, Function, and Bioinformatics, 2002
AbstractEnzymes adapted to cold display structures comparable with those of their meso‐ and thermophilic homologs but are characterized by a higher catalytic efficiency at low temperatures and by thermolability at moderate temperatures. To identify the structural factors responsible of such features, we undertook a systematic comparative analysis of ...
GIANESE, Giulio   +2 more
openaire   +4 more sources

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