Results 151 to 160 of about 532,623 (184)

Psychrophilic Enzymes: Potential Biocatalysts for Food Processing

2019
Babu Joseph
exaly   +2 more sources

Extracellular proteolytic enzymes of psychrophilic bacteria. I. Purification and some properties of enzymes of an obligately psychrophilic red-pigmented bacterium

Canadian Journal of Microbiology, 1968
Proteinase in culture fluids of an obligately psychrophilic bacterium was precipitated by ammonium sulfate and fractionated by gel filtration and DEAE-cellulose chromatography. Three purified fractions (I-1, I-2, and III-1) with proteinase activity were obtained. On the basis of reactions and characteristics (i.e. effect of pH, heat, and metal ions on
Y, Nunokawa, I J, McDonald
openaire   +2 more sources

Psychrophiles as a novel and promising source of cold-adapted industrial enzymes

The Applied Biology & Chemistry Journal, 2023
Psychrophiles are an exclusive group of microbes that thrive in extremely cold environments, such as polar regions and deep-sea. These cold-loving microbes have developed a range of adaptations that enable them to function at low temperatures, including the production of cold-adapted enzymes.
Varsha Gupta, Pranav Bhaskar, Jeancolar Thoudam, Shiwali Bisht, Anita Sharma, Rashmi Tripathi   +5 more
openaire   +1 more source

Extracellular proteolytic enzymes of psychrophilic bacteria. II. Production and heterogeneity of enzymes of an obligately psychrophilic red-pigmented bacterium and of a white variant

Canadian Journal of Microbiology, 1968
The appearance of proteolytic enzymes in culture fluids during growth of an obligately psychrophilic red-pigmented bacterium and of a white variant was followed. Enzymes were precipitated with ammonium sulfate and fractionated on Sephadex G-100. Both organisms produced a slow-moving component (III) at the start of proteinase production and a fast ...
Y, Nunokawa, I J, McDonald
openaire   +2 more sources

Molecular Biology Applications of Psychrophilic Enzymes: Adaptations, Advantages, Expression, and Prospective

Applied Biochemistry and Biotechnology
Psychrophilic enzymes are primarily produced by microorganisms from extremely low-temperature environments which are known as psychrophiles. Their high efficiency at low temperatures and easy heat inactivation property have attracted extensive attention from various food and industrial bioprocesses.
Hu Xu, Yongqin Liu
exaly   +3 more sources

Molecular adaptations of enzymes from psychrophilic organisms

Comparative Biochemistry and Physiology Part A: Physiology, 1997
Abstract The dominating adaptative character of enzymes from cold-evolving organisms is their high turnover number (kcat) and catalytic efficiency ( k cat K m ), which compensate for the reduction of chemical reaction rates inherent to low temperatures.
Feller, Georges, Arpigny, J. L., Narinx, E., Gerday, Charles   +3 more
openaire   +1 more source

Directed evolution study of temperature adaptation in a psychrophilic enzyme.

Journal of molecular biology, 2000
We have used laboratory evolution methods to enhance the thermostability and activity of the psychrophilic protease subtilisin S41, with the goal of investigating the mechanisms by which this enzyme can adapt to different selection pressures. A combined strategy of random mutagenesis, saturation mutagenesis and in vitro recombination (DNA shuffling ...
Miyazaki, Kentaro, Wintrode, Patrick L., Grayling, Rowan A., Rubingh, Donn N., Arnold, Frances H.   +4 more
openaire   +3 more sources

Isolation and characterization of psychrophilic yeasts producing cold-adapted pectinolytic enzymes

Letters in Applied Microbiology, 2004
The present study was conducted to screen for psychrophilic yeasts that are able to degrade pectin compounds at low temperature, and to examine the cold-active pectinolytic enzymes produced by the isolated psychrophilic yeasts.Psychrophilic yeasts, which grow on pectin as a sole carbon source, pectinolytic-psychrophilic yeast (PPY) strains PPY-3, 4, 5 ...
T, Nakagawa, T, Nagaoka, S, Taniguchi, T, Miyaji, N, Tomizuka   +4 more
exaly   +3 more sources

Thermolability of glycolysis enzymes from a psychrophilic marine bacterium

Journal of Oceanography, 1977
Cell extracts ofVibrio marinus MP-1 grown at 15°C and suspended in either tris-HCl buffer or NaCl were compared for heat lability of hexokinase, glucose phosphate isomerase, and aldolase. In tris buffer glucose phosphate isomerase and aldolase lost 50 per cent activity after one hour at low thermal exposures of 34°C and 28°C, respectively.
Richard Y. Morita, Paul F. Mathemeier
openaire   +1 more source

Two distinct arginine kinases in Neocaridina denticulate: Psychrophilic and mesophilic enzymes

International Journal of Biological Macromolecules, 2014
The small shrimp Neocaridina denticulata has two types of arginine kinases (AKs): AK1 and AK2. We determined the full kinetic parameters for the forward reaction of the AKs at temperatures between 15 and 35°C. The catalytic efficiencies, determined by kcat/(Ka(ATP)·Kia(ARG)), of the two enzymes showed a marked difference in temperature dependence.
Tomohiko, Suzuki, Yoshitaka, Kanou
openaire   +2 more sources